EMAGE mouse gene expression spatial database

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EMAGE:11650

Orai2 ORAI calcium release-activated calcium modulator 2 ( MGI:2443195)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008399. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008399_01	euxassay_008399_02	euxassay_008399_03	euxassay_008399_04

euxassay_008399_05	euxassay_008399_06	euxassay_008399_07	euxassay_008399_08	euxassay_008399_09

euxassay_008399_10	euxassay_008399_11	euxassay_008399_12	euxassay_008399_13	euxassay_008399_14

euxassay_008399_15	euxassay_008399_16	euxassay_008399_17	euxassay_008399_18	euxassay_008399_19

euxassay_008399_20	euxassay_008399_21	euxassay_008399_22	euxassay_008399_23	euxassay_008399_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11650_wholemount_strong.wlz

11650_wholemount_moderate.wlz

11650_wholemount_weak.wlz

11650_wholemount_possible.wlz

11650_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11650_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thyroid gland

moderate

regional

moderate expression: see section 11 12 16 17

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 06 07 08 09 moderate expression: see section 05 10 21 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 09 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v nerve

weak

regional

weak expression: see section 10 18

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 17 18 19

neural retina

moderate

regional

moderate expression: see section 02 03 04 24 weak expression: see section 01

naris

strong

regional

strong expression: see section 13 moderate expression: see section 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 16 17 18 moderate expression: see section 09 15 19 20

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 12 13 16 17

tongue

moderate

regional

moderate expression: see section 14

lower jaw incisor

strong

regional

strong expression: see section 12 13 16 17

lower jaw molar

strong

regional

strong expression: see section 08

upper jaw incisor

strong

regional

strong expression: see section 12 13 16 17

upper jaw molar

strong

regional

strong expression: see section 08 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9375
Entity Detected:	Orai2, ORAI calcium release-activated calcium modulator 2 ( MGI:2443195)
Sequence:	sense strand is shown >T9375 ACAGACGCTAGCCACGAGCCGGAGCCGTGAGCTGGAGCCAAGCGCGCTGAGGGCTGGACAGTCAGGCCTG GTCCCCACCATGAGTGCAGAGCTCAATGTGCCCATGGACCCCTCTGCCCCTGCCTGCCCTGAACCCGGCC ACAAGGGCATGGATTACCGAGACTGGGTCCGGCGCAGCTACCTGGAACTCGTCACGTCTAACCACCATTC GGTGCAGGCACTGTCCTGGAGGAAGCTCTACCTGAGCAGGGCCAAGCTGAAAGCCTCCAGCCGGACCTCA GCCCTCCTGTCTGGCTTCGCCATGGTGGCCATGGTGGAGGTGCAGCTGGAGACCAAGTACCAGTACCCTC AGCCCCTGCTCATTGCCTTCAGCGCCTGCACCACGGTGCTGGTAGCCGTGCATCTGTTTGCTCTGCTCAT CAGCACTTGCATCCTGCCCAACGTGGAAGCGGTGAGCAACATCCACAACCTCAACTCCATCAGCGAGTCG CCGCACGAGCGCATGCACCCGTACATCGAGCTGGCCTGGGGCTTCTCCACCGTTCTGGGCATCCTGCTTT TCCTGGCTGAGGTGGTCCTGCTCTGCTGGATCAAGTTCCTCCCAGTGGATGCCAAGGATCAGCCAGGCTC CCACAGCCACACGGGCTGGCAGGCCGCTCTGGTGTCCACCATCATCATGGTACCTGTGGGTCTCATCTTC GTGGTCTTCACCATCCACTTCTACCGTTCCCTGGTGCGGCACAAGACGGAGCGCCACAACCGTGAGATCG AAGAGCTGCACAAGCTCAAGGTGCAGCTGGATGGGCACGAGCGGAGCCTGCAGGTGGTGTGAGGGAGGCC GTTGCGATTTGCTACCAACCTGCACGGGAGGGCAACCCTCAGGCCACCACGCAGCTGGTCAGAGTAGCCA AGGGGGCCGCCAGACAAACCCGGGCTGCATGAGATCCACCACACATCCCAAGAGCCACAGTGTGGGGATG GCTAAACAGACTGCCTGGATGCTGCTCCAATAATCCCATACTCCTGTCCCCGCATGAATAAGGGTCTAAA AGGAGTGAGAAAGCGTCCGTTTCATCATGGAGGCCGGAGGAAGAAACACGACCACGTGGCCCTGGGGACT TCCTGGTCCAATCATATCAGAGAGCACTGTAGATCTCAGACGTCTGGACCTTGTGATTGGCAGGGCAGGG TGGTGAATTCCCAAAGCCAGCTGGGGGCTGGGGCTCAGGTGGGAGAGTGTTTGCCTAGTGTGCAGGAAGC ACTGCCTAAGCCAAGTCATCCCAGGGTCATCGAGGTGCTAGGGAGGTGGAGGAAGGCAGATCAGGGCTTC AAAGTTGTTCTTTGCTACATGGTCAGTTTGAGGCTACACGAAAAAGTCAGACACATGCACATGCATGCAG AAGAGCGCGCACGTGCACACACGTGCGTACACACGCACACACATGCACACAGACACACACAAAGAAGGGG CTGCTTGAGTGTGCAGAGGTGGGCCCGGGGCTGGCACAGGGTAACATGGCTTATGATGAAAGACAGGGAT GTTTTACCAGATCTGTAGCTCAGCTTGGCTCTTTCCTCCCGATTGGAAAGGGTCACAGAGTGTTCAGGAC CCTGGGGCACACAGCAGCTCAACAAGGGTAACAGATACTGTTATTGGAGTGAGAGTTTCCTGGTTCTAGA GAGACAGCCATAGGCCTGAGGGCAGCCATGCCACCCCCACCCCCCATGGACTGTGTCAACGCAGGCAGGG GCACCATCTCAGAGACCAGGCAGCTCAGATCCCTTCTGCCTGTGGCCCCCAGATGTTGAGAGGATAAACT GGGGCGACTTCCTGTTTTTGCAAAGTGGTTAGCCCTGTGGTTAGGCCTGTGGGTGGGGCAGGGACCTGGC CCAGCCTAATATAGGCAGCTATTCCCTGTAATGAGGCTATGGGCAGAGAAGGGGAGACTTCAACACCCCA TTTCTGGGCCTACCAGACACTCCATGCAGGTCCCTGCCCCGTGTTTACACAGCTGTGTATGGGTTTCGCA TTGCCACTACGGGCATCTGGCTATCCAGCTGGTATTGTCATGCATACTGTGCTATACCACACCAGCTTGC GTGGGGGCCAGTACTTACCCATTCTGTCACATTACCTAGAGGGCTGAGGTCTCTTACTCTGTATATAAAT GGTCGCTGGGCTCCTGCGAGTACCACTTCCATCTCTTACAACAGCTTTTCTTTGGAGTCTGGATTTGGTG CCCGTGGGATCCTGGAGGGCCCCACCCCCATCCTGAAGTCTCTGGAAATGGGTCATACTGCCAAGCATAA ACTTCAACAGGCAGAGGGCCCTGGGGAGCCTGGTGGAGGTCTTCCTTGTAAAGTGTGACATCAGTGGACA GCCAGGACATAGTGTCCTGTGAACCCAGGAGAAACGCCCTCTGTCTTTCAAAGGCTGTATCCTTCAACAC TCTCAGCCCTTGCACACATCTGTCTGAGACCTCTTGCGTTTCAGATCTCTGTTTTCCTGGGGTCTTTTTG GGAAGGCCAGCCTAGTTTGATTTGTTTGTCTTGCTAAAGAATGCTATAATCCTGGGTGGACAAGATGGCT CAGTGGGTAAAGGCGCTTGCCACCAAGCCTGGAGACCTGAGTTTAGTCCCAATCCTCTGACCCACAACCA AGTAAATATAACGAGAAAAACGATTTACTGTGAGCATCACTGTAAGCTGGTGTGCGAGCAGGGGAGGAGA AGATGACCTCTGGAAGGAATCCACACATGGCCAGGCACATCTGTAAGGTAAACCTTCAAGGGGTGGAGGC AGGAGGATCAGGGGTTCAAGGCCAGCTTGGGCTGGACAGACTCTATCAGGAAAAGGGGGTGCTGCAGATG ACCCCCGTATTTAGGAATGCTTGCTGCTCTTGCAGAGGACCAGAGTTCATCTCCCAGCACCTATGTAGGG CAGCTCACAACGGCCTGTGACTCAAGTTCTAGAGGATACAACACCCTCTTCTGGCCTCTACAGGCACCCA CTCACATGTCCATATTTACACATATAAAATAAAAAAGGGGATTCCTGCTAAACATTGAGTCCACTCCTTC TACCCATTTTCTGAAGCCATTTGGTGCCTCTGCTCATGGGGGCACCCGGCACTAGTGCAGTAGGGGACTC CTGCCATTCTTCACGTTCTACCATTAGTCTTCCTAGCGCTGAGCATGGGACAGCCATCTGTGTATGAACA GAGGTGTCAGCCCATGTGGTCACCCATGGGCAGGAGTCACAGGGCCATCCCACAGCTTTGTCAGGAGTTG GTGGATAAGCCCTCCCTGCCACCAGTGGCGGAAGCCAGCAAGCTCAAGGCTGGAAGTTCAGTTTCTGGAG GCATAACCTGATTTTTGAATCTTGTTCTAACTGCAGCCACGTGGCTAGAACCACTGGGGGCTCCTGGGAC AGGCACAGGCTCGCCCTAATTTCATCTATCCAAGATTCAGCCCTTCCTGGTGCTCAAGGGACCCGTGTCC TCAGACACACCAAGGCCCTTCCCAACACGGCCTCCCCGCACCCTCAACCACAAAGTCTCTAAATTCAAGC GTTTCTACTTAAGACATTTCAAAGGCAGAACATGATTGGTGTCTTTTGGGAAAGGCTACTGAAAACAAAC TACAGGAGCCTGGCGGGCTCAAGACTATATCATTATTTCTGCAGGACACTGTAAATAACTCCTTTCCTGG GTGTGTGGTTTTTTGTTTGTTTGTTTGTTGATTTTTTTTTGCAGTTGCCCCGGATTGAGCTGAATAAACT GGTACAGATTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6853893 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6853893 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11649 same embryo
	EMAGE:11648 same embryo
	EMAGE:11652 same embryo
	EMAGE:11651 same embryo
	EMAGE:11647 same embryo
	EurExpress:euxassay_008399 same experiment
	MGI:4826980 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source