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EMAGE:14197

S100a14 S100 calcium binding protein A14 ( MGI:1913416)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197
"Pseudo-wholemount" of euxassay_003998. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003998_01 euxassay_003998_02 euxassay_003998_03 euxassay_003998_04
EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197
euxassay_003998_05 euxassay_003998_06 euxassay_003998_07 euxassay_003998_08 euxassay_003998_09
EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197
euxassay_003998_10 euxassay_003998_11 euxassay_003998_12 euxassay_003998_13 euxassay_003998_14
EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197
euxassay_003998_15 euxassay_003998_16 euxassay_003998_17 euxassay_003998_18 euxassay_003998_19
EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197 EMAGE:14197
euxassay_003998_20 euxassay_003998_21 euxassay_003998_22 euxassay_003998_23 euxassay_003998_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14197Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14197_wholemount_strong.wlz
14197_wholemount_moderate.wlz
14197_wholemount_weak.wlz
14197_wholemount_possible.wlz
14197_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14197_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
single cellmoderate expression: see section 09 10 12 13
epidermis
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 09 10 11 12 13 14 15 16 17 18 19 20 24 moderate expression: see section 07 08
vibrissa
moderate moderate
regionalmoderate expression: see section 06 07 08 weak expression: see section 05
nasal cavity respiratory epithelium
strong strong
regionalstrong expression: see section 13 14 16 17
esophagus
strong strong
regionalstrong expression: see section 10 11
oral epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 06 07
bladder
moderate moderate
regionalExpression in the fundus region.
urethra of male
weak weak
regionalweak expression: see section 11 12
rectum lumen
moderate moderate
regionalmoderate expression: see section 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5000
Entity Detected:S100a14, S100 calcium binding protein A14 ( MGI:1913416)
Sequence:sense strand is shown

>T5000
CCACGCGTCCGCGGACGCGTGGGCGGACGCGTGGGCCCAGCTGCCAGCAGGTAGATCATGATCCATCAGC
TCCTGTGGGGCAGGCTATAGGACAGACGACAAAACTCAACTCACAGAAGGAAGGACCAGTGTACCAGGAA
CGATGGGACAGTGTCGGTCAGCCAATGCTGAGGATGCCCAAGAATTCAGTGATGTAGAGAGGGCCATTGA
GACACTCATCAAGAACTTCCATAAATACTCTGTGGCGGGTAAAAAGGAAACACTGACCCCTGCTGAGCTT
CGAGACCTGGTTACCCAGCAGCTGCCACACCTCATGCCGAGCAACTGTGGGTTAGAAGAGAAAATTGCCA
ACCTGGGCAACTGTAATGACTCGAAACTGGAGTTTGGAAGCTTCTGGGAGTTGATTGGAGAAGCAGCCAA
GAGTGTGAAGATGGAGAGGCCTGTTACTCGGAGCTGAGGACTTCTACTTGGAACTTGTTGGGGGTGTTGG
GGATAGGGGAGTTTTAGAGGCACTGGAAATAAAACCCTCAATGCCCACCACCCCCTTCCCCAGCCTGCAC
CTCTCCTCATTGCTGCAATGTTCACGTTCAGGACAGGCTTCCCTGTGGGCTCCATGGAGCTCCTGGGTCC
AGAAGTCCTCATCTCAAGGGAGCTCAGGGGGTGGGTTGGGGCTGGAGAGGATATGCAGGGATCCTGGAAG
GGTAAGGGCCAAGCAATTTGGTAGTAGGGGAAGGGCAGAAAGGAACTGGGTTATGGAAGTGATCCAAAGA
GCAGGGATGGGAATCTGGCTGCATATTTGGTCCTGAAAAGGGTGTCTGAGAACCTACCCCCTTCTAATCT
TGTCCCACCTAAACTGTAGTTGTCTGCCCTGTGCTATCCTTGCTGCTTCCAGCTCTGCCCCATCCTCCTT
CCAGTGTCTGTTCCTGAGTAGGGGCAGGGGAAATAGGAGCAGAGTTGCAAAAGAGGCTGAGGAGGGCATG
ACTTCATCACTTTGGGGTGAGAGGACCAGCTAGATGCTTGGGCATTTATGGTAGTTATTTTATATCATTT
GATTAATAAAAATATTGGAAAATGTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5151924 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5151924 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14195 same embryo
 EMAGE:14200 same embryo
 EMAGE:14199 same embryo
 EMAGE:14198 same embryo
 EMAGE:14196 same embryo
 EurExpress:euxassay_003998 same experiment
 MGI:4827849 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS