EMAGE mouse gene expression spatial database

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EMAGE:14198

Gdi1 guanosine diphosphate (GDP) dissociation inhibitor 1 ( MGI:99846)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004022. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004022_01	euxassay_004022_02	euxassay_004022_03	euxassay_004022_04

euxassay_004022_05	euxassay_004022_06	euxassay_004022_07	euxassay_004022_08	euxassay_004022_09

euxassay_004022_10	euxassay_004022_11	euxassay_004022_12	euxassay_004022_13	euxassay_004022_14

euxassay_004022_15	euxassay_004022_16	euxassay_004022_17	euxassay_004022_18	euxassay_004022_19

euxassay_004022_20	euxassay_004022_21	euxassay_004022_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14198_wholemount_strong.wlz

14198_wholemount_moderate.wlz

14198_wholemount_weak.wlz

14198_wholemount_possible.wlz

14198_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14198_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 15 16 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 16 17

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 13

cervical ganglion

moderate

regional

moderate expression: see section 07 14

thoracic ganglion

moderate

regional

moderate expression: see section 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 13 14

retina

moderate

regional

moderate expression: see section 02 03 04

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5001
Entity Detected:	Gdi1, guanosine diphosphate (GDP) dissociation inhibitor 1 ( MGI:99846)
Sequence:	sense strand is shown >T5001 CCACGCGTCCGGCAGCAGCAGCAGCGGCAGTAGCAGCAGTGGCAATAGTAGAGGTAGTGGTAGCGGCTGC TGTGGTGAAGGAGGAGGAGCAGAGCGGACGCTGGTACCAAGGCCTGATCATGGATGAGGAATACGATGTG ATTGTGCTGGGGACAGGTCTTACCGAATGCATCCTGTCTGGCATCATGTCTGTGAATGGAAAGAAGGTGC TGCACATGGACCGAAACCCCTACTATGGGGGTGAGAGCTCTTCCATCACACCCCTGGAGGAGCTATATAA GCGCTTTCAGATACTGGAAGGGCCCCCTGAATCAATGGGCCGGGGCCGAGACTGGAATGTTGACTTGATC CCCAAATTCCTCATGGCCAATGGTCAGCTGGTAAAGATGCTACTATATACAGAAGTGACTCGATATCTGG ACTTCAAGGTGGTAGAGGGCAGCTTTGTATATAAGGGGGGCAAGATCTACAAAGTGCCATCCACCGAGAC TGAGGCCTTGGCTTCTAATCTGATGGGCATGTTTGAAAAACGACGCTTCCGAAAATTTTTGGTGTTTGTG GCAAACTTTGATGAGAATGACCCCAAAACCTTTGAGGGTGTTGATCCCCAGAACACCAGCATGCGTGATG TCTACCGGAAGTTTGACCTGGGTCAAGATGTCATAGATTTCACTGGCCATGCCTTGGCGCTCTACCGCAC TGATGACTACCTGGATCAGCCCTGTCTTGAGACTATTAACCGCATCAAGTTGTACAGCGAGTCCCTGGCC CGGTATGGCAAGAGCCCCTATTTATACCCACTGTATGGCCTGGGTGAGCTGCCCCAGGGCTTTGCCAGAT TGAGTGCCATCTATGGGGGAACATACATGCTGAACAAACCAGTGGATGACATCATCATGGAAAATGGCAA GGTGGTGGGTGTCAAATCTGAGGGAGAGGTGGCCCGCTGCAAGCAGCTGATCTGTGATCCCAGTTACATC CCAGACCGTGTGCAAAAGGCTGGCCAGGTTATTCGCATCATCTGTATCCTCAGCCACCCCATCAAGAATA CCAATGATGCCAATTCCTGCCAAATTATCATCCCTCAGAACCAGGTCAACAGGAAGTCAGACATCTATGT GTGCATGATCTCCTATGCGCACAACGTGGCCGCACAGGGCAAATACATCGCCATTGCCAGCACCACTGTA GAGACTGCAGAACCAGAAAAGGAAGTTGAGCCTGCATTGGAGCTGTTGGAACCCATTGACCAGAAGTTTG TGGCTATCAGTGATTTATATGAGCCCATTGATGATGGTTCAGAGAGTCAGGTGTTCTGTTCCTGCTCCTA TGATGCTACCACACACTTTGAGACAACCTGCAATGACATCAAAGATATCTACAAACGCATGGCTGGTTCT GCCTTTGATTTTGAGAACATGAAGCGCAAACAGAATGATGTCTTTGGAGAAGCTGATCAGTGATTGCTGA CTCCCCTCCTCCAGCCCCTGTTCCCCTCTCCCCAACTTGTATGTCCTTCTCTAGGGCAGGGGAGATGGTA TGGCTAGGCATCCTTGTTTCCAGCATCTTCTGCTTCCCCTACCACATTCCAATCACCCACCTTATTGATT TTATTCACTGACCATATCCTTAACCTTAGGGATGGTTTGGGAGATGGGAGTTGGATAGTATCCTCTTTCT TGGTCTGTCCTTGTTCTAGGGAAAGAGAACTTTGTGTGTGTGTGTGTGTGTGCGCGCGCGCGTGCGTGTG TGTACGTGTGTGTACGTACGTGTGTGTGCGTGTGTACATGTGTGTGCATGTGTGTGTGCATCGTGCACCC CCAATTTTGCTGTTTGGGAAGGAGTGGAGGAAAAGCTGACCCCCACCCCCACTGCTCTTAGTCCCAAATG TAGTGGCCTTTGGAGATACCCATCTCCTCCCCCAACTCTTAGGTGTTGGAGAGAAGGGACCCTCCCAGCA CAAAATTGCATTCCTCCTCCTCCTCCTCCTCCTTATAATTTATTCTAATTTATTAACTTTGGAGTAATTG TCTGAGCCCTCAGCCTTTCTGTGCAGCCTAGGGCTGTAGAGTGAGCTGCCCTGGCCTCTCCCAGTGCTGG CAAAGCCTGGGATGGGAGAAGATTTAGGTGTGACCCCCTCTGGAGGTTGATCTGTTTTGAATTTCTTTTG TGTTGTGTTCACTGAGAAAAAGTAATGTGAGCAAAGCAGAAGGAGGTAGGAAAGTGGATCCAAACCCCAG TGTACCCTGCCCCATGCCTTTTCCTTTAGTGGTGGGAAACCCTTACCTTGCAAAGTGAATGTGTCCCCTT CCCCAAGCTCTAGTATATTTCACAGAAAACAAAAACCTCCCAATAAAACTATTGAAACTTAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5249271 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5249271 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14195 same embryo
	EMAGE:14200 same embryo
	EMAGE:14197 same embryo
	EMAGE:14199 same embryo
	EMAGE:14196 same embryo
	EurExpress:euxassay_004022 same experiment
	MGI:4825036 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source