EMAGE mouse gene expression spatial database

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EMAGE:14332

Gfra1 glial cell line derived neurotrophic factor family receptor alpha 1 ( MGI:1100842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004165. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004165_01	euxassay_004165_02	euxassay_004165_03	euxassay_004165_04

euxassay_004165_05	euxassay_004165_06	euxassay_004165_07	euxassay_004165_08	euxassay_004165_09

euxassay_004165_10	euxassay_004165_11	euxassay_004165_12	euxassay_004165_13	euxassay_004165_14

euxassay_004165_15	euxassay_004165_16	euxassay_004165_17	euxassay_004165_18	euxassay_004165_19

euxassay_004165_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14332_wholemount_strong.wlz

14332_wholemount_moderate.wlz

14332_wholemount_weak.wlz

14332_wholemount_possible.wlz

14332_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14332_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper leg muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 17 18 19

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 02 03

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 06 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata alar plate marginal layer

strong

regional

strong expression: see section 07

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

pons mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 15 16 17 18 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 15 16 17

spinal cord mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 15 16

aorta

strong

regional

strong expression: see section 12

esophagus

strong

regional

strong expression: see section 12 13 moderate expression: see section 11

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09

rectum

strong

regional

strong expression: see section 12

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

lower lip

strong

regional

strong expression: see section 03 04 05 06 07 08 13 14 15 16 17 18

upper lip

strong

regional

strong expression: see section 03 04 05 06 15 16 17 18 19

bladder

strong

regional

strong expression: see section 11 12

renal cortex

strong

regional

strong expression: see section 06 07 08 09 10 16 17 18 19

left lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11

right lung

moderate

regional

moderate expression: see section 12 13 14 15 16

axial skeleton

moderate

regional

moderate expression: see section 11 12 13 14

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14

axial skeleton tail region

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7846
Entity Detected:	Gfra1, glial cell line derived neurotrophic factor family receptor alpha 1 ( MGI:1100842)
Sequence:	sense strand is shown >T7846 CTTTGAGTGGCCAGAGGAGCGCAGTCGCCCGGGGATCTCTGCACGCTGAGCTCTCTCCGCGAGATCCGGT GGCGGCTTTGGATTTTGGGGGGGCGGGGACCAGCTGCGCGGCGGCACCATGTTCCTAGCCACTCTGTACT TCGTGCTGCCACTCCTGGATTTGCTGATGTCGGCCGAGGTGAGTGGTGGGGACCGCCTGGACTGTGTGAA AGCCAGTGATCAGTGCCTGAAGGAACAGAGCTGCAGCACCAAGTACCGCACACTGAGGCAGTGCGTGGCG GGCAAGGAAACCAACTTCAGCCTGACATCCGGCCTCGAGGCCAAGGATGAGTGCCGCAGCGCTATGGAGG CCTTGAAGCAGAAGTCTCTCTACAACTGCCGCTGCAAGCGGGGCATGAAGAAAGAGAAGAATTGTCTGCG TATCTACTGGAGCATGTACCAGAGCCTGCAGGGAAATGACCTACTGGAAGATTCCCCATATGAGCCGGTT AACAGCAGGCTGTCAGATATATTCCGGGCAGTCCCGTTCATATCAGATGTTTTCCAGCAAGTGGAACACA TTTCCAAAGGGAACAACTGCCTCGATGCAGCCAAGGCCTGCAACCTGGATGACACCTGCAAGAAGTACAG GTCCGCCTACATCACCCCCTGTACCACCAGCATGTCCAATGAAGTCTGCAACCGCCGCAAGTGCCACAAA GCCCTCAGGCAGTTCTTCGACAAAGTTCCAGCCAAGCACAGCTACGGGATGCTCTTCTGCTCCTGCCGGG ACGTCGCCTGCACCGAGAGGCGGCGACAGACTATCGTCCCTGTGTGCTCCTATGAAGAACGAGAGAGGCC CAACTGCCTGAATCTGCAAGACTCCTGCAAGACAAATTACATCTGCAGATCTCGCCTTGCAGATTTTTTT ACCAACTGCCAGCCAGAGTCAAGGTCTGTCAGCAACTGTCTTAAGGAGAACTACGCAGACTGCCTCCTGG CCTACTCGGGACTGATTGGCACAGTCATGACTCCTAACTACATAGACTCCAGCAGCCTCAGTGTGGCGCC GTGGTGCGATTGCAGCAACAGTGGCAATGACCTGGAAGATTGCCTGAAGTTTCTGAATTTTTTTAAGGAC AATACGTGTCTCAAAAATGCAATTCAAGCCTTTGGCAATGGCTCGGATGTGACCATGTGGCAGCCAGCCC CCCCAGTCCAGACCACCACTGCCACGACTACCACTGCCTTCCGGATCAAGAACAAGCCTCTAGGGCCAGC AGGCTCTGAGAATGAGATTCCCACACACGTTTTACCACCGTGTGCTAATTTGCAGGCACAGAAGCTGAAA TCCAATGTATCGGGCAGTACACATCTCTGTCTTTCTGATAATGATTACGGAAAGGATGGTCTCGCTGGTG CCTCCAGCCACATAACCACAAAATCAATGGCTGCTCCTCCCAGCTGCGGTCTGAGCTCACTGCCGGTGAT GGTGTTCACCGCTCTGGCTGCCCTGTTGTCTGTATCATTGGCAGAAACATCGTAGCTGCATCCAGTATGA AAAGACAAAAGAAAACCAAGTATTCTGTCCCCTGTCCTCTTGTATATCTGAAAATCCAGTTTTAAAAGCT CGGTTGAGAAGCAGTTTCACCCAGCGGGAACTCCTTTGTTGTTTTTAAGAAAGCTTTTTGCCGCCCTCAC AGGCTTCTGTTGAAGAACTGCTACAGGGCTAATTCCAAACTCAGAAAACTCTGGGGCATGGTGTGGCTTC AGGGGACTGTTTGTAACCTGGACTGTAAAGCAAGCTGAGCTTATGTTTTTGATGGTGATGGTGGTGGTGA TGATGAAGATGATAGTTTTAACAGCTTGAACCCTGTTCTCTCTACTGGCTAGGAGGAGGAGATGCTATTG ATAGAGATTCTTCCGGATCTTATTTAATAGCGTTGGATTCTAAAGACATGACTGCAGCCTAGTGTGGATG GATGACAATTGGGGGTTGGCCTCAAGAGTGGACTTTGCAGACTCTATCTTGTACTAACGTTCACCGTTCT ATGTATGCTCTCCACAGAGTGTTTATGTGATCCCCAACAAAACAAAAAAGTCCCTGGTCACATCCAGATG TAGAAGCTACCATTTAGCCCAGTAGAGGAGAGTGGGTGTGGTCCTTGCACAGCACCCGGGATTGTTCACT GGTGCGCTCTCCTGAGCTTTGCTTGAGTGAGAAGCTGAATGTAGCTGAAGATCAACTCTTACCACTTCTT ACACTTACGAGGTCACATGTAGAACAAGCATTACCAACTGTCAGCTCTCCAACTGTTAGCTTTCTACATT TTGAAGGCAGCGATACCGGTCCCATGTTTTAATGATGGTTTAATACACACAGCCCTTGCCCCTCATCAGT AATACTAGCTCCAGCATCAACTTCATGCATATGGCTCTCATTAAAAGCAGGCCCCCAAGAAGCCCAGTGC TTTAGCAGAAAGCCACATCACATTTCTGTGGACAGGCAGGATGAAATAGAGCAGCCAGCCCATGTCTCAT CTGTCTTGAGATCAAGGCTGCCTATATGTAAGGAATGGTTAGATACTTGTAAAGGGTGCCACAGTAGACA GCGCTTCTGGCAGTTGATATAGCCCCAGTACATGGGTTTTCATGTTGTCTGGCAGAGACAGCTTGGATTA GGACTGGCTGGGATGGAGATATGCTGCCCTCTCTTTCACAGTTGAGTCCTGCCAGGGCACATGGAAGTTT CTTTGCTTTTGACCACAACTTAGAACAGTTTGGATTCAAGGTCACCAAGTATCTCCTGTATTTTGCTCTG TGTCCTTCATGACAGACTGTCACACACAAAAGGAATGGTAGGTTATGGATCTAGAGGGCCAGCACAGGGT CTGGGAAGGTGAACTGAATGGCACAAGTACACAGAGCAGGAGGGGACGCTCGAGAAAAGACACACACACC ATCTCCTGGAGCCCTCCTCCTTGCCTGGGCAGACCCTTAGGCATATATCTAAAGACTGCTTTACATGGTC AGGCTCAGAATTTGTTAATGGTTGCGTACTGGCAACCATGTTTCCTGCAAAGTGAATAAGGGGGGGGGGG GAGAATCTTCCTTCCCTTGAGCCCTAGAAGTATGATCTGGCCCCTCACCCTCCCACAGAGCAGGGAGCCC TGGTGCACGGTCTCCTGATACCCGCTCTGCTCTCTGAGGTTTGCCTTTGGGATAATAAATAACGATTCAC CTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6390018 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6390018 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14330 same embryo
	EMAGE:14329 same embryo
	EMAGE:14328 same embryo
	EMAGE:14331 same embryo
	EurExpress:euxassay_004165 same experiment
	MGI:4825047 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source