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EMAGE:14437

Mapk13 mitogen-activated protein kinase 13 ( MGI:1346864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437
"Pseudo-wholemount" of euxassay_004253. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004253_01 euxassay_004253_02 euxassay_004253_03 euxassay_004253_04
EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437
euxassay_004253_05 euxassay_004253_06 euxassay_004253_07 euxassay_004253_08 euxassay_004253_09
EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437
euxassay_004253_10 euxassay_004253_11 euxassay_004253_12 euxassay_004253_13 euxassay_004253_14
EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437
euxassay_004253_15 euxassay_004253_16 euxassay_004253_17 euxassay_004253_18 euxassay_004253_19
EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437 EMAGE:14437
euxassay_004253_20 euxassay_004253_21 euxassay_004253_22 euxassay_004253_23 euxassay_004253_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14437Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14437_wholemount_strong.wlz
14437_wholemount_moderate.wlz
14437_wholemount_weak.wlz
14437_wholemount_possible.wlz
14437_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14437_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
adrenal gland
moderate moderate
regionalmoderate expression: see section 09 10 11
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 15 16 17 18 19
pancreas
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13
adenohypophysis
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16
physiological umbilical hernia epidermis
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vibrissa
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 19 20 21 22
ear
moderate moderate
regionalmoderate expression: see section 24
inner ear
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 17 18 19 20
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 02 24
cornea
moderate moderate
regionalmoderate expression: see section 04 05 24 weak expression: see section 01 02 03
naris
moderate moderate
regionalmoderate expression: see section 10 11 16 17
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 15 16 17 18 19 20
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 13 15
esophagus epithelium
moderate moderate
regionalmoderate expression: see section 11
pharynx epithelium
moderate moderate
regionalmoderate expression: see section 10 11 12 13
tongue epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16
stomach
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11
rectum
moderate moderate
regionalmoderate expression: see section 17
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 09 10 11 14 15 16
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 19
oral epithelium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 15 16
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 19
bladder
moderate moderate
regionalmoderate expression: see section 13 14 15
metanephros
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 19 20
female reproductive system
moderate moderate
regionalmoderate expression: see section 16 17
lung
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 22 23 24
trachea
moderate moderate
regionalmoderate expression: see section 12 13
temporal bone
moderate moderate
regionalmoderate expression: see section 06
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6094
Entity Detected:Mapk13, mitogen-activated protein kinase 13 ( MGI:1346864)
Sequence:sense strand is shown

>T6094
GGCCGGGAGCCCGCGGGCAGCCACCGCAACCTCCTCGCAGGACCGCCACCACGGGGTCCGGGATGAGCCT
CACTCGGAAAAGGGGCTTCTACAAGCAGGACATCAACAAGACTGCCTGGGAGCTACCCAAGACCTACCTG
GCGCCCGCGCACGTCGGCAGCGGGGCCTATGGAGCGGTGTGCTCGGCCATCGACAAGCGGACAGGGGAGA
AGGTGGCCATCAAGAAGCTGAGCCGGCCCTTCCAGTCGGAGATCTTTGCCAAGCGCGCTTACCGCGAGCT
TCTGCTCTTGAAGCACATGCACCATGAGAACGTCATTGGGCTTCTGGATGTCTTCACCCCTGCGTCTTCC
CTTCGGAGCTTCCATGATTTCTACCTGGTGATGCCCTTCATGCAGACAGACCTACAGAAGATCATGGGGA
TGGAATTCAGCGAGGATAAGGTCCAGTACTTGGTGTACCAGATGCTCAAAGGTCTAAAGTACATCCACTC
CGCTGGCATCGTCCACAGGGACCTGAAACCGGGCAACCTGGCTGTGAATGAAGACTGTGAGCTGAAGATC
CTGGACTTTGGGCTGGCCCGCCACACAGACACTGAGATGACGGGCTATGTGGTGACCCGCTGGTACCGGG
CCCCCGAGGTGATCCTCAGCTGGATGCATTACAACCAGACAGTCGACATCTGGTCTGTTGGTTGCATCAT
GGCAGAAATGCTGACTGGAAAGACACTCTTCAAGGGCAAGGACTACCTGGACCAGCTGACCCAGATCCTG
AAAGTGACTGGGGTGCCAGGTGCCGAGTTCGTGCAGAAGCTGAAAGACAAGGCGGCCAAATCCTATATTC
AGTCCCTGCCCCAGAGCCCCAAGAAGGATTTCACACAGCTCTTTCCACGCGCCAGTCCGCAAGCTGCAGA
CCTGCTCGACAAGATGCTGGAGCTGGATGTGGACAAGCGTCTGACCGCTGCTCAGGCACTGGCTCACCCC
TTCTTTGAACCCTTCCGGGACCCTGAGGAGGAGACAGAGGCCCAGCAGCCTTTTGATGATGCCTTAGAAC
ATGAGAAACTCAGTGTGGACGAATGGAAACAACACATCTACAAAGAGATCTCAAACTTCAGTCCCATAGC
CCGGAAGGACTCACGGCGACGAAGTGGCATGAAGCTGCAGTGACTGATGGCTGGCCTCCATGTAGCCCAG
GTTGGCCTCCATGTAGCCCAGGCTGGCCTTGAGTCTTCCTCCTCTCCTTTTTTGAGTCAGGATCTTACTG
TGTAGCCCTGGAGCCCTGGCTAGACCTGCAACTCAAGAGATCCACCCACCTCTGTTACCTGGGTGGTGGG
ATTAAAGGCATGCACCACTGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3492903 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3492903 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14442 same embryo
 EMAGE:14441 same embryo
 EMAGE:14439 same embryo
 EMAGE:14440 same embryo
 EMAGE:14438 same embryo
 EurExpress:euxassay_004253 same experiment
 MGI:4826089 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS