EMAGE mouse gene expression spatial database

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EMAGE:17109

Rad51 RAD51 homolog (S. cerevisiae) ( MGI:97890)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001498. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001498_01	euxassay_001498_02	euxassay_001498_03	euxassay_001498_04

euxassay_001498_05	euxassay_001498_06	euxassay_001498_07	euxassay_001498_08	euxassay_001498_09

euxassay_001498_10	euxassay_001498_11	euxassay_001498_12	euxassay_001498_13	euxassay_001498_14

euxassay_001498_15	euxassay_001498_16	euxassay_001498_17	euxassay_001498_18	euxassay_001498_19

euxassay_001498_20	euxassay_001498_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17109_wholemount_strong.wlz

17109_wholemount_moderate.wlz

17109_wholemount_weak.wlz

17109_wholemount_possible.wlz

17109_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17109_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12 13

hypothalamus ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 09 10

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 09 10

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 06 07 08 11 12 13

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 05 06 07 15 moderate expression: see section 12 13 14

medulla oblongata basal plate marginal layer

moderate

regional

moderate expression: see section 11

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10

rest of cerebellum marginal layer

strong

regional

strong expression: see section 19 moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 19 moderate expression: see section 02 03 04 05 06 07 08 09 16 17 18

pons ventricular layer

moderate

regional

moderate expression: see section 07 08 09 11 12 13

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 13

cervical ganglion

moderate

regional

moderate expression: see section 07 14

thoracic ganglion

moderate

regional

moderate expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4489
Entity Detected:	Rad51, RAD51 homolog (S. cerevisiae) ( MGI:97890)
Sequence:	sense strand is shown >T4489 CGCAGATCCGCGCCTGGGCAAAGCCGAGGCGCTCCCACAGGTGTGGCCAGGAAGACTGAAATTTTTCGTA GAAAACCTGACAGAGGAGCAGCGAAGCGCGTTCGAGCCGGGTGAAGTGGGGGGAGTGTGGTGTGTGCAGA GCCGGCTGCCCGGAGGAGCGAGCGGCCCGTGGCCGCGGGACAGTCATGGCTATGCAAATGCAGCTTGAAG CAAGTGCAGATACTTCAGTGGAAGAAGAAAGTTTTGGTCCACAGCCTATTTCACGGTTAGAGCAGTGTGG CATAAATGCCAATGATGTGAAGAAATTAGAAGAAGCCGGTTACCATACAGTGGAGGCTGTTGCTTATGCA CCGAAGAAGGAACTAATAAATATTAAGGGAATTAGTGAAGCCAAAGCTGACAAAATTCTGACTGAGGCAG CAAAATTGGTTCCAATGGGTTTCACCACGGCAACTGAGTTTCACCAGCGCCGGTCAGAGATCATACAGAT AACTACTGGCTCCAAAGAGCTGGACAAGCTGCTTCAAGGTGGAATTGAGACTGGATCTATCACAGAGATG TTTGGAGAATTCCGAACTGGGAAGACACAGATCTGTCATACGTTGGCTGTTACATGCCAGCTCCCCATTG ACCGTGGTGGAGGTGAAGGGAAGGCCATGTACATTGACACCGAGGGCACCTTTAGGCCGGAGCGGCTGCT AGCAGTAGCTGAGAGATACGGTCTCTCTGGCAGCGATGTCCTAGATAATGTAGCATATGCGCGAGGGTTC AACACAGACCACCAGACCCAGCTCCTTTACCAAGCGTCAGCCATGATGGTAGAATCCAGGTATGCACTGC TTATTGTAGACAGTGCTACTGCCCTTTACAGAACAGACTACTCAGGGCGGGGAGAGCTTTCAGCCAGGCA AATGCATTTGGCCAGATTTCTGAGGATGCTGCTTCGACTTGCTGATGAGTTTGGTGTCGCAGTGGTAATC ACCAACCAGGTAGTAGCCCAAGTAGATGGAGCAGCCATGTTCGCTGCAGATCCCAAAAAACCCATTGGAG GGAACATCATCGCTCATGCGTCAACCACCAGGCTGTACCTGAGAAAAGGAAGAGGGGAGACCAGAATCTG CAAAATCTATGACTCTCCCTGTCTTCCTGAAGCTGAAGCTATGTTTGCCATTAATGCAGATGGAGTGGGC GATGCCAAAGACTGACTCCTTAGGCTTTCTTTCCTGTGATGCTATACGGCTTTTCTAAATGCTCTACAGC TAATGGAGAGGACTGTGTACTGGGCTTCTTCACAGGCTCATGTTGTGACTGCCCCGCATGGCTTCCGGGA AAACAACTATTGTATCAGCTCTCTGGTAGCAGAAACACATCTGATCTGAAAGGTTTCCTGTGGAGTCTAT TAATAACTGATTTCTTTGGTGTTTAGAGGAGAAGTATGAAATAGCAGGTGTGGGGCAGTCTTTTGTCTTC AGGAGAAGTAAACTGGAAAGATCCGAGTACTCTCACTTCTCAAGGATGGTAAACTAAATGCCTGAGGATC TGTAAGTGGGAATGGGTGGTTTACACATTCTTTTCTCTGTAAGACAGATGCAAACTTAGTTATATGCCTG AACTGTCACATCATTTTAAAATTTATTTTTGATTAAGGAGTCTTACATGCAGCATGCTAGACTACGATCC CTGCATGCTTGTTCTCTAAATTACTAAGACTTCACTTAAGGAAATTTGTTCTGCTGCTAATAAAGTAGTT GTGTGCAGTGGATGTGTTATTTGCTGGAGATATTCTAGTGCAAACTGAATATTGCAAAACAGGGTTTCTA TAACAGCTTTCGGTACTGTGTCGTTCGTTATGGTCAGCTGCAGTTGTAAAGTGTGAACCTGAAAGAAAGC CCAGCATTCAGGTTACTGCATTGTAGCCTTTGGCCGAAGAATAAGAACCCCAGGCTGGTTTACTGTGTCT TTCCTGTGGTCTTTGTTTTTATTGTATTAGTTTTGTGTTGCAGAGGTCTGACATTACTCAAATAACTCTG CATCAGGAGTATTTCCAAAAACTATAGTCTTCTTTTGAGTCATTGAGTCTCATTATTAAAAGAATTTCAA AACAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4951015 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4951015 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17106 same embryo
	EMAGE:17107 same embryo
	EMAGE:17111 same embryo
	EMAGE:17108 same embryo
	EMAGE:17110 same embryo
	EurExpress:euxassay_001498 same experiment
	MGI:4827590 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source