EMAGE mouse gene expression spatial database

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EMAGE:17428

Col18a1 collagen, type XVIII, alpha 1 ( MGI:88451)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003864. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003864_01	euxassay_003864_02	euxassay_003864_03	euxassay_003864_04

euxassay_003864_05	euxassay_003864_06	euxassay_003864_07	euxassay_003864_08	euxassay_003864_09

euxassay_003864_10	euxassay_003864_11	euxassay_003864_12	euxassay_003864_13	euxassay_003864_14

euxassay_003864_15	euxassay_003864_16	euxassay_003864_17	euxassay_003864_18	euxassay_003864_19

euxassay_003864_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17428_wholemount_strong.wlz

17428_wholemount_moderate.wlz

17428_wholemount_weak.wlz

17428_wholemount_possible.wlz

17428_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17428_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12

brain

moderate

single cell

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

adenohypophysis

strong

regional

strong expression: see section 10 11 12 13 14

physiological umbilical hernia epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon meninges

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

telencephalon meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

hindbrain meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

spinal cord

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 11 not examined expression: see section 20

spinal cord meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12

cochlea

strong

regional

strong expression: see section 03 04 05 06 07 08 14 15 16 17 18 19 20

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 20

cornea

moderate

regional

moderate expression: see section 05

retina

moderate

regional

moderate expression: see section 01 02 03 04 05

naris

strong

regional

strong expression: see section 14 18

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 16 17 18 19 20

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 14 15 16 17 18

heart

strong

regional

strong expression: see section 10 11 12 moderate expression: see section 05 06 07 08 09 13 14 15

tongue

moderate

regional

moderate expression: see section 12 13 14 15 16

lower jaw incisor

strong

regional

strong expression: see section 11 12

lower jaw molar

strong

regional

strong expression: see section 07 19

upper jaw incisor

strong

regional

strong expression: see section 12

upper jaw molar

strong

regional

strong expression: see section 07 19

bladder

moderate

regional

moderate expression: see section 11 12

metanephros

moderate

regional

moderate expression: see section 04 05 06 07 13 14 15

genital tubercle of male

moderate

regional

moderate expression: see section 11 12 13 14

urethra of male

moderate

regional

moderate expression: see section 11 12

not examined

regional

not examined expression: see section 15

lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7815
Entity Detected:	Col18a1, collagen, type XVIII, alpha 1 ( MGI:88451)
Sequence:	sense strand is shown >T7815 TCACCGAGGAGCTGGGTGCAGCATCCGGGCGCAGACGGCCTGGCCTGGGGAGATGGCGCCCAGGTGGCAC CTCCTGGATGTGCTCACCAGTTTGGTCTTGCTGCTGGTGGCACGCGTCTCCTGGGCAGAGCCAGAGAATG TTGCTGAGGAGGTGGGGCTGCTGCAGCTCCTTGGAGACCCCCTACCTGAGAAGATCTCACAAATCGATGA CCCTCACGTCGGGCCGGCCTACATCTTTGGACCAGACTCCAACAGTGGCCAGGTGGCCCAGTATCATTTC CCAAAACTCTTCTTCCGGGACTTTTCGCTGCTGTTTCATGTCCGGCCAGCCACAGAGGCAGCAGGGGTGC TATTTGCCATCACAGATGCTGCCCAGGTGGTAGTCTCACTGGGCGTGAAGCTCTCAGAGGTCCGAGATGG ACAGCAAAACATCTCATTGCTCTACACGGAGCCTGGGGCCAGCCAGACCCAGACGGGAGCCAGCTTCCGC CTACCTGCATTTGTTGGGCAGTGGACACACTTCGCGCTCAGCGTCGACGGAGGCTCTGTGGCTCTCTACG TAGACTGTGAAGAATTCCAGAGGGTGCCATTTGCTCGGGCCTCGCAGGGACTGGAGCTAGAGCGTGGCGC TGGCCTCTTTGTGGGTCAGGCTGGAACAGCAGACCCTGACAAGTTCCAGGGGATGATCTCAGAGCTGAAG GTACGCAAAACCCCCCGGGTGAGCCCTGTGCACTGTCTGGATGAAGAAGATGATGATGAAGACCGGGCAT CTGGAGATTTTGGAAGTGGCTTTGAAGAAAGCAGCAAGTCACACAAGGAGGATACATCTCTACTACCTGG GCTCCCTCAGCCACCTCCTGTCACTTCCCCACCCCTGGCTGGAGGCAGCACCACAGAAGATCCTAGAACA GAAGAAACGGAGGAAGACGCCGCGGTAGATTCTATAGGAGCTGAGACCCTTCCTGGCACAGGTTCAAGCG GTGCATGGGATGAGGCTATCCAGAACCCCGGAAGGGGCTTGATAAAGGGAGGTATGAAAGGACAAAAGGG AGAACCAGGTGCCCAGGGCCCACCTGGCCCAGCTGGGCCCCAGGGTCCTGCCGGTCCAGTGGTCCAGAGC CCCAACTCACAACCTGTCCCTGGAGCACAAGGACCCCCGGGACCTCAGGGGCCACCAGGGAAGGATGGCA CTCCAGGAAGGGATGGTGAACCGGGTGACCCTGGTGAAGATGGGAGACCGGGTGACACTGGACCTCAAGG CTTTCCAGGGACCCCAGGAGATGTGGGCCCTAAGGGCGAGAAGGGAGATCCTGGTATTGGGCCCCGAGGA CCTCCAGGGCCTCCAGGGCCACCAGGACCCTCCTTCAGACAAGACAAGCTGACCTTCATTGACATGGAGG GATCCGGTTTCAGCGGAGACATAGAGAGCCTTAGAGGCCCACGAGGCTTCCCTGGCCCCCCGGGGCCCCC TGGTGTCCCAGGACTTCCTGGTGAGCCAGGACGCTTTGGGATCAATGGTTCCTATGCACCAGGACCTGCA GGCCTTCCTGGTGTACCTGGGAAGGAAGGACCCCCCGGTTTTCCAGGTCCCCCGGGACCTCCAGGTCCTC CAGGCAAAGAGGGCCCACCAGGAGTGGCCGGCCAGAAAGGCAGTGTTGGTGATGTGGGCATCCCAGGACC CAAGGGGAGCAAAGGAGACCTTGGGCCCATCGGTATGCCTGGCAAGTCTGGCTTGGCTGGATCCCCTGGG CCAGTTGGACCCCCAGGACCTCCAGGGCCTCCAGGGCCACCAGGACCAGGATTTGCTGCTGGATTCGATG ATATGGAAGGCTCTGGAATACCCCTCTGGACAACAGCCCGAAGCTCTGATGGGCTGCAGGGACCTCCCGG GTCGCCGGGACTCAAGGGGGATCCTGGAGTGGCAGGCCTACCTGGAGCCAAGGGAGAAGTTGGAGCAGAT GGAGCCCAGGGCATCCCTGGTCCCCCAGGAAGAGAAGGTGCAGCTGGATCTCCGGGGCCAAAAGGAGAGA AAGGGATGCCGGGAGAAAAGGGAAACCCAGGAAAAGATGGAGTGGGCCGGCCGGGCCTCCCTGGGCCTCC AGGACCTCCAGGGCCTGTGATCTATGTGTCAAGTGAGGATAAAGCAATAGTGAGCACGCCAGGACCTGAG GGCAAGCCAGGGTACGCAGGCTTTCCTGGACCTGCTGGACCGAAGGGTGACCTGGGTTCCAAAGGCGAGC AGGGTCTTCCGGGGCCCAAGGGTGAGAAGGGAGAGCCAGGCACTATCTTTAGTCCTGATGGCAGAGCTCT GGGCCATCCCCAGAAGGGAGCCAAGGGAGAGCCAGGCTTTCGAGGACCCCCGGGTCCTTATGGACGACCT GGGCACAAGGGTGAAATTGGCTTCCCTGGACGGCCGGGTCGACCTGGAACGAATGGCTTAAAGGGAGAGA AAGGAGAGCCTGGAGATGCCAGCCTTGGGTTCAGCATGAGGGGATTGCCTGGCCCCCCTGGGCCTCCAGG ACCCCCAGGTCCTCCTGGGATGCCCATCTATGACAGCAATGCATTTGTGGAGTCTGGCCGACCTGGACTA CCAGGACAGCAGGGTGTGCAGGGGCCTTCAGGACCAAAGGGTGACAAAGGAGAGGTGGGCCCACCTGGGC CACCAGGGCAATTCCCCATTGACCTCTTCCACCTGGAAGCGGAAATGAAGGGGGACAAGGGAGACCGAGG GGATGCTGGACAGAAAGGAGAGAGGGGAGAACCTGGGGCTCCTGGTGGTGGATTCTTCAGCTCAAGTGTA CCTGGCCCACCCGGCCCACCTGGATACCCTGGAATTCCGGGTCCAAAGGGAGAGAGCATCCGGGGGCCAC CTGGCCCTCCTGGCCCGCAGGGACCTCCTGGCATTGGCTATGAGGGTCGCCAGGGTCCCCCAGGACCTCC GGGACCTCCAGGACCTCCCTCCTTCCCTGGCCCTCACAGACAGACTGTCAGTGTTCCTGGTCCTCCGGGC CCACCTGGTCCTCCAGGTCCCCCAGGAGCCATGGGTGCCTCTGCTGGGCAGGTGAGGATCTGGGCCACAT ACCAGACCATGCTGGACAAGATCCGGGAGGTGCCGGAGGGCTGGCTCATCTTTGTGGCCGAGAGGGAAGA GCTCTATGTACGCGTTAGAAATGGCTTCCGGAAGGTGCTGCTGGAGGCCCGGACAGCCCTCCCGAGAGGC ACGGGCAATGAGGTGGCTGCTTTGCAGCCCCCATTGGTCCAGCTTCATGAGGGCAGTCCATACACCCGGA GGGAGTACTCCTATTCCACGGCACGACCCTGGCGAGCAGATGACATCCTGGCCAACCCACCGCGCCTGCC AGACCGCCAGCCTTACCCCGGAGTTCCACATCACCACAGTTCCTATGTGCACCTGCCGCCAGCCCGCCCC ACCCTCTCACTTGCTCATACTCATCAGGACTTTCAGCCAGTGCTCCACCTGGTGGCACTGAACACCCCCC TGTCTGGAGGCATGCGTGGTATCCGTGGAGCAGATTTCCAGTGCTTCCAGCAAGCCCGAGCCGTGGGGCT GTCGGGCACCTTCCGGGCTTTCCTGTCCTCTAGGCTGCAGGATCTCTATAGCATCGTGCGCCGTGCTGAC CGGGGGTCTGTGCCCATCGTCAACCTGAAGGACGAGGTGCTATCTCCCAGCTGGGACTCCCTGTTTTCTG GCTCCCAGGGTCAACTGCAACCCGGGGCCCGCATCTTTTCTTTTGACGGCAGAGATGTCCTGAGACACCC AGCCTGGCCGCAGAAGAGCGTATGGCACGGCTCGGACCCCAGTGGGCGGAGGCTGATGGAGAGTTACTGT GAGACATGGCGAACTGAAACTACTGGGGCTACAGGTCAGGCCTCCTCCCTGCTGTCAGGCAGGCTCCTGG AACAGAAAGCTGCGAGCTGCCACAACAGCTACATCGTCCTGTGCATTGAGAATAGCTTCATGACCTCTTT CTCCAAATAGGGCCTCTGCCAGCTAGGGTGGCAGACAGAGGCCATGCAGAACTTTGACACAGCGCAGGGA GCATTCAGTCAGCACCCAGGGCTCTGGCTGGGATACAATCCTGTATAGTTCCCATTTTTATGTAATCCTC AAGAAATAAAAGGAAGCCAAAGAGTATATTTTTCTAAAACATTTACAACAGACTTCCTGCCCTTCCTCTC CCACTTGAACTGGCCAGTCTGTGTCACAGTCCCTTCTTCCTCCGTGCTTGGTAGAGCTTCCTGGCGAAGT CGGACCACAGCTAGGTCTCCACCGGAGGCGATGCCCTACAGTTGCTGCCTCAGACCTGCAGACTCTATCT AGCCTGGGATCTTTCTGTGCAGATCGTTCATACAACAGTCACATGCCATGTGCTGGGGACAGCACAGGGC AGGCAGTCCAAGGCTCCCTTCTCACCGCCCAGTGGCTGTGCCAGGCATATAGGCGGGCACCTGCTCATCC AGGTGGTAACTGGGTCTACTCATCCTTGAGCAGTGGGAACCAGCTCTCCTGACTGCCCCACTCTCCTGCA GCCACACTCCCTGCCAGGGGAGGTTCCCAAGCTCAGTAACTCACTGTGACCCCAGATCTGACCCCCCCTC CCGTCACCATGGGCATAGGCCAGGAATGTGGCCAGACTTAGCCTGCGTGCTCATTTTTCCGAGGGCCAGA CGTGACATGGGGCCCATCCTCAGGTTTGTCTCTCAGACTGGCCTCTCTGGCGACCCCTGAGATCCTGTTC AGAGAACCCAACCCCAACCAAGCCCAAGCTGGACATCAGCTTGAAGTCCAGAAATCTCACAGCAGCCACA TGAAGCACTTGTCCTATGAAGGGACTTGTGTGCCCAGGGTCACAAGTGGCCAATCAAAGCCACTGTGCCT GCCTCAGGCTCTGCTGTCCAAGGATTGGGACAATCCAGTGGGTCTGGTTCTGTGACAATGTGTGCTATGA TGCCATTTCAAAGAGGCACCAAATAAAAATGACATTTTATACCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6332464 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6332464 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17429 same embryo
	EMAGE:17433 same embryo
	EMAGE:17432 same embryo
	EMAGE:17431 same embryo
	EMAGE:17430 same embryo
	EurExpress:euxassay_003864 same experiment
	MGI:4823982 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source