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EMAGE:17433

Hmga2 high mobility group AT-hook 2 ( MGI:101761)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17433 EMAGE:17433 EMAGE:17433 EMAGE:17433 EMAGE:17433
"Pseudo-wholemount" of euxassay_003865. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003865_01 euxassay_003865_02 euxassay_003865_03 euxassay_003865_04
EMAGE:17433 EMAGE:17433 EMAGE:17433 EMAGE:17433 EMAGE:17433
euxassay_003865_05 euxassay_003865_06 euxassay_003865_07 euxassay_003865_08 euxassay_003865_09
EMAGE:17433 EMAGE:17433 EMAGE:17433 EMAGE:17433 EMAGE:17433
euxassay_003865_10 euxassay_003865_11 euxassay_003865_12 euxassay_003865_13 euxassay_003865_14
EMAGE:17433 EMAGE:17433 EMAGE:17433 EMAGE:17433 EMAGE:17433
euxassay_003865_15 euxassay_003865_16 euxassay_003865_17 euxassay_003865_18 euxassay_003865_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17433Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17433_wholemount_strong.wlz
17433_wholemount_moderate.wlz
17433_wholemount_weak.wlz
17433_wholemount_possible.wlz
17433_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17433_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 15 16 17 18 19 weak expression: see section 01 02 03
humerus
moderate moderate
regionalmoderate expression: see section 02 03 04 18 19 weak expression: see section 01
hand
moderate moderate
regionalmoderate expression: see section 01 02 04
hindlimb digit 1 metatarsal
moderate moderate
regionalmoderate expression: see section 03 04
hindlimb digit 1 phalanx
moderate moderate
regionalmoderate expression: see section 03 04 05
hindlimb digit 2 metatarsal
moderate moderate
regionalmoderate expression: see section 03 04
hindlimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 03 04 05
hindlimb digit 3 metatarsal
moderate moderate
regionalmoderate expression: see section 03 04
hindlimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 03 04 05
hindlimb digit 4 metatarsal
moderate moderate
regionalmoderate expression: see section 04
hindlimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 04 05
hindlimb digit 5 metatarsal
moderate moderate
regionalmoderate expression: see section 04
hindlimb digit 5 phalanx
moderate moderate
regionalmoderate expression: see section 04 05
fibula
moderate moderate
regionalmoderate expression: see section 01 02
tibia
moderate moderate
regionalmoderate expression: see section 01 02
adrenal cortex
moderate moderate
regionalmoderate expression: see section 05 06 07 13 14
thymus primordium
strong strong
regionalstrong expression: see section 09 moderate expression: see section 10 11 12
sublingual gland primordium
moderate moderate
regionalmoderate expression: see section 08 09 16
pancreas
weak weak
regionalweak expression: see section 07 08 09 10
thyroid gland
strong strong
regionalstrong expression: see section 09 moderate expression: see section 08
pituitary gland
strong strong
regionalstrong expression: see section 10 11 12 13
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 12 13 14
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 12 13 14
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 13 17 18
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 09 10 11 12 moderate expression: see section 05 06 07 08 13 14 15 16 17 18 19 weak expression: see section 03 04
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 10 17 18 weak expression: see section 03 04 05 06 07 11 12 13 14 15 16
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 05 06 07 11 12 13
cochlea
moderate moderate
regionalmoderate expression: see section 05 06 07 08 13 14 15 16
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
esophagus
moderate moderate
regionalmoderate expression: see section 10
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07
hindgut
moderate moderate
regionalmoderate expression: see section 11 12
midgut
moderate moderate
regionalmoderate expression: see section 11 12 14
metanephros
moderate moderate
regionalmoderate expression: see section 05 06 07 08 13 14 15
lung
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18
trachea
strong strong
regionalstrong expression: see section 10 11
axial skeleton
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12
pelvic girdle skeleton
weak weak
regionalweak expression: see section 08 14 15
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7814
Entity Detected:Hmga2, high mobility group AT-hook 2 ( MGI:101761)
Sequence:sense strand is shown

>T7814
CCGTCTCCCGAAAGGTGCTGGGCGGATTCGGGGCGGCGGAGGCCGAGCGGCTGCAGCGGCGGTACCGGTA
GAGGCAGTGGTAGCGGCGGCGGGAGGCAGGATGAGCGCACGCGGTGAGGGCGCCGGGCAGCCGTCCACAT
CAGCCCAGGGACAACCTGCCGCCCCGGTGCCACAGAAGCGAGGACGCGGCCGACCCAGGAAGCAGCAGCA
AGAGCCAACCTGTGAGCCCTCTCCTAAGAGACCCAGAGGAAGACCCAAAGGCAGCAAAAACAAGAGCCCC
TCTAAAGCAGCCCAGAAGAAAGCAGAGACCATTGGAGAAAAACGGCCAAGAGGCAGACCTAGGAAATGGC
CACAACAAGTCGTTCAGAAGAAGCCTGCTCAGGGGGGTGAGTTTGGAGAACGCACCAGGTGCCATGAATC
ACAAACCTCAAGTCTGCCCCACAATCCTTATGGGCAGGAGCTCATCGCTTCCACTCTCATGGGAGACTGA
AGAGACATCCTCGCAAGAGTCCGCAGAGGAGGATTAGGGGGCGCCGACATTCAATTTCTACCTCAGCATC
AGTTGGATCTTTTGAAGGGAGAAGACACTGCAGTGACCAGTTATTCTTAACTGCCACGGTCTTTCTACTT
CCTGCGGGGTGGGGCGGGGGCGGGGCTGGGCGAGGGGCGGGGCCGGGGTGGGCGAAATCGCATAACCTTG
AGAAGGACTATATTAATCACTTTGTAATCCCTTCACAGTCCCAGGTTTAGTGAAAAACTGCTGTAAACAC
GGGGGACACAGTTTAACAATGCAACTTTTAATGACTGTTTTCTTTTTCCTTAACTTACTAATAGTTTGTG
GATCTGATAAGCAAGGGTGTGTGGTTGAAGAAAACCTCTGTGGTGGGCTTAATCAGTCACTACATGCAAA
CCCTAAACCGGCACCCTGGTGACCGGGGGCATTCGTATAAGAAAAGCATTGTGTGTGACTCTGTGTCCAC
TCAGATGCCACCCCCACCATGATCATAGAAAATCTGCTTAGGACACCAAAGATGAGAACTAGACACTACT
CTCCTTTCTTTGTGTATAATCTTGTAGACACTTACTTGATTTTTTTTTCTTTTTTTACTTTTCAATTCTG
AATGAGACAAAATGCTGGTGTATCTTTTCATACAGCTAGCAAACCAGAATAGGTTATGCTCGTTTTTTGC
TTTGTTTTGTTTTTCAAAAAGGGAAGTAAACGAGAACCGTTGACTCCTCCATTTATGGACTCATACACAG
CAGCAGGAGTGATAAGCCCACAAGCTCTCTTTCCCGCCTCGGGAAATCTACACAGCCAAAAGCCACTTAG
CCATAAATGACACTTGTCAGCCTTGAAGCATCGGAGATAACTAGCTGAGTAAAATGATCCTGTTTTGGAA
TTTAATGAAAAGGTTAACAGTACCCAATGAACCCACCCAAGTGATGACATGGGAGGAGCGAAACCGAAAT
CTCTTTTGCTATATAAAGGACACTATTTTTTAAAAAAAAATAATAAAAACAGCTCCCGCTCTCTGTCCTC
TCTCCCTCCCTTTTTTCCCTCGCCTCTCTCTCCTCTCTATATTCCCTGTTCTTCATTGTGTACCAGTGTC
CGTGAAAGACCGCAGTACCACTTACCTCAGATGAAGCCTGCGTGTTACATCCTGTAACACCTTTCATTTT
GACATAAGATGGCTAGCCGAGGTGCATTATCTTGGTTCGGACTGCCATCTCTGCATTCACGCTGCACTTT
TAGCCAGAGATGCAATAATCCCCACTCCTCAATACTACCTCTGAATGCTACAGTGAATTTACAGCCCTGC
ACTTGTTACACGCTGCTAGACACAAGCCCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6332337 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6332337 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17429 same embryo
 EMAGE:17428 same embryo
 EMAGE:17432 same embryo
 EMAGE:17431 same embryo
 EMAGE:17430 same embryo
 EurExpress:euxassay_003865 same experiment
 MGI:4825390 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS