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EMAGE:18218

Cops7b COP9 (constitutive photomorphogenic) homolog, subunit 7b (Arabidopsis thaliana) ( MGI:1349388)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218
"Pseudo-wholemount" of euxassay_011995. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011995_01 euxassay_011995_02 euxassay_011995_03 euxassay_011995_04
EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218
euxassay_011995_05 euxassay_011995_06 euxassay_011995_07 euxassay_011995_08 euxassay_011995_09
EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218
euxassay_011995_10 euxassay_011995_11 euxassay_011995_12 euxassay_011995_13 euxassay_011995_14
EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218
euxassay_011995_15 euxassay_011995_16 euxassay_011995_17 euxassay_011995_18 euxassay_011995_19
EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218 EMAGE:18218
euxassay_011995_20 euxassay_011995_21 euxassay_011995_22 euxassay_011995_23 euxassay_011995_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18218Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18218_wholemount_strong.wlz
18218_wholemount_moderate.wlz
18218_wholemount_weak.wlz
18218_wholemount_possible.wlz
18218_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18218_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 04 20 22 23 24
fibula
moderate moderate
regionalmoderate expression: see section 02 24
tibia
moderate moderate
regionalmoderate expression: see section 02 24
femur
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 24
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 16 17
axial skeleton
moderate moderate
regionalmoderate expression: see section 06 07 09 10 11 12 13
axial skeleton cervical region
moderate moderate
regionalmoderate expression: see section 08
basioccipital bone
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 18 19
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 19 20 21 22 23
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 19 20
scapula
moderate moderate
regionalmoderate expression: see section 02 03 04 20 22
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 08 09 10 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30082
Entity Detected:Cops7b, COP9 (constitutive photomorphogenic) homolog, subunit 7b (Arabidopsis thaliana) ( MGI:1349388)
Sequence:sense strand is shown

>T30082
ACGGCTGTGAGGCAGTTCTCTTGGGCATTGAGCAGCAGGTTCTGAGAGCCAACCAGTACAAAGAGAACCA
CCACCGGACTCAGCAGCAGGTGGAAGCCGAGGTTAGCAACATTAAGAAGACACTCAAAGCAACAGCATCC
TCCTCAGCTCAGGAAATGGAGCAGCAGCTGGCTGAACGGGAGTGTCCCCCCCATACTGAGCAGAGGCAAC
CCACTAAGAAGATGTCCAAAGTGAAAGGTCTGGTCTCCAGCCGCCACTAGGGCCGGCTGGGGCAGCTGGC
ACTCAGCAGGCCTGGGTCAGGTCGGGAGGGGACACCCAGGGCCTATTTCCGCCCCTCTCTAACTGCAGTG
AGTTCCAGATCTGCCCACCCTTCACCAGTACCTCCCCATCCATTGGTACCATTGCGGAAAATGATTGCTC
CTCCCTTGCCTCCTTCCCTAGTTGTTCCCTTCAGACTCAGGGGCTCAATGGATGCCATCGTAACAGGGTC
CAGCACTGCCCAGCTTCCCTGCAGGGGTCCTTGTCTCTGTGATGGGCTACCTCCCTCCCAGTTGTTCTTT
GCTCTCTGTCATTTTGTCAGGCTGGTCCTAAGCTGGAGGCAGTTTACCCAGCCCCAAGGGATGACTGTGG
AGGTGGCTCCTCTCTGAGTGAAGAGGGGGCCTTGCTTCTCCAGCCCCATGTACCACAGTATCCACAATGG
TGCAAGTGTCTCTAGCCAGGTAGCCACATGTCCCCTCTCCTGCCTTACCCCTGTTGGCAGTGCCAGTTGA
GGCCATGGAGGGATGTGGTGCTGGGCTATGTTTACTAAACCTTTGGGTTTTCAGCCTCTTAAGCCCAGCC
AGGATCTCTCATCAAGTTGGGGCACTGGGCTGACTAACCCCTGGTGTCTGACACCAGTGGAGGGTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4017012), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16815. Forward Primer - name:016815_F_IRAV20-23_H21_Cops7b, sequence:ACGGCTGTGAGGCAGTTC; Reverse Primer - name:016815_R_SP6_IRAV20-23_H21_Cops7b, sequence:GCACCCTCCACTGGTGTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18217 same embryo
 EMAGE:18220 same embryo
 EMAGE:18215 same embryo
 EMAGE:18219 same embryo
 EMAGE:18216 same embryo
 EurExpress:euxassay_011995 same experiment
 MGI:4824008 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS