EMAGE mouse gene expression spatial database

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EMAGE:18245

Tmsb15l thymosin beta 15b like ( MGI:3026988)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012030_01	euxassay_012030_02	euxassay_012030_03	euxassay_012030_04	euxassay_012030_05

euxassay_012030_06	euxassay_012030_07	euxassay_012030_08	euxassay_012030_09	euxassay_012030_10

euxassay_012030_11	euxassay_012030_12	euxassay_012030_13	euxassay_012030_14	euxassay_012030_15

euxassay_012030_16	euxassay_012030_17	euxassay_012030_18	euxassay_012030_19	euxassay_012030_20

euxassay_012030_21	euxassay_012030_22	euxassay_012030_23	euxassay_012030_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18245_wholemount_strong.wlz

18245_wholemount_moderate.wlz

18245_wholemount_weak.wlz

18245_wholemount_possible.wlz

18245_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18245_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 05 06 07 08 09 12 19 20 21

radius

weak

regional

weak expression: see section 02 03

ulna

weak

regional

weak expression: see section 02

humerus

weak

regional

weak expression: see section 01 02 03 04 05 06 22 23 24

hip

weak

regional

weak expression: see section 12 13 19 20

fibula

weak

regional

weak expression: see section 05 07 08

tibia

weak

regional

weak expression: see section 04 05 06 07 08 09 10

femur

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 23 24

otic capsule

weak

regional

weak expression: see section 06 07 08 09 10 17 18

meckel's cartilage

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22 23

axial skeleton

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18

basioccipital bone

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

basisphenoid bone

weak

regional

weak expression: see section 11 12 13 15 16

exoccipital bone

weak

regional

weak expression: see section 01 02 03 04 05 17 18 19 20 21 22 23 24

temporal bone

weak

regional

weak expression: see section 01 02 03 04 20 22 23 24

orbito-sphenoid

weak

regional

weak expression: see section 02 03 07 08 09 10 22 23

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 01 02 03 04 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8360
Entity Detected:	Tmsb15l, thymosin beta 15b like ( MGI:3026988)
Sequence:	sense strand is shown >T8360 GCACGAGGGGACATAACTGCTGGCCTGAAGGGAGCCTTGGAACCGGCAGACAAGATGAGCGATAAACCAG ACTTGTCAGAAGTTGAAACGTTTGACAAATCAAAGTTGAAGAAAACTAATACTGAAGTAAAAAATACTCT TCCGTCGAATGAAAACAAGATGAGCGATAAACCAGACTTGTCAGAAGTTGAAACATTTGACAAAGCAAAG TTGAAGAAAACTAATACTGAAGTAAAAAATACTCTTCCGTCGAAGGAAACTATCCAGCAAGAGAAAGAAC ACAATGAAAGAACATAAAATGAGACTCTCCTTTCAAGAGCAACAACAACATTGCCTAACAGTCATGGCTC TAGGCTTGTTTCTGTAACCCTATGCAATATATAGACATGTTAGGCAGCTCCTGGTAGATCCATTTACTAC CCTGGCTGCAAAGGTCAGCATTTGCTATGAATCATTAAATTTTTTTGGTACTGCTCAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3373135 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3373135 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18247 same embryo
	EMAGE:18246 same embryo
	EMAGE:18248 same embryo
	EMAGE:18250 same embryo
	EMAGE:18249 same embryo
	EurExpress:euxassay_012030 same experiment
	MGI:4828831 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source