EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19341

Slc4a8 solute carrier family 4 (anion exchanger), member 8 ( MGI:1928745)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002110. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002110_01	euxassay_002110_02	euxassay_002110_03	euxassay_002110_04

euxassay_002110_05	euxassay_002110_06	euxassay_002110_07	euxassay_002110_08	euxassay_002110_09

euxassay_002110_10	euxassay_002110_11	euxassay_002110_12	euxassay_002110_13	euxassay_002110_14

euxassay_002110_15	euxassay_002110_16	euxassay_002110_17

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19341_wholemount_strong.wlz

19341_wholemount_moderate.wlz

19341_wholemount_weak.wlz

19341_wholemount_possible.wlz

19341_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19341_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 02 03 04 08 09

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 13 moderate expression: see section 01 02 03 04 12

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 02 03 10

trigeminal v ganglion

strong

regional

strong expression: see section 13 14 moderate expression: see section 01 02 03 04 05 11 12 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 02 03 04 10 11

spinal cord

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 04 07 08

cervical ganglion

moderate

regional

moderate expression: see section 04 09

thoracic ganglion

moderate

regional

moderate expression: see section 06

dorsal root ganglion

moderate

regional

moderate expression: see section 01 02 03 04 06 07 08 09

not examined

regional

not examined expression: see section 01 02 03

neural retina

moderate

regional

moderate expression: see section 01 02 03

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 moderate expression: see section 06 07 08 10 11 12 13 14

kidney calyx

weak

regional

weak expression: see section 02 03 08 10 11

kidney pelvis

weak

regional

weak expression: see section 04 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5493
Entity Detected:	Slc4a8, solute carrier family 4 (anion exchanger), member 8 ( MGI:1928745)
Sequence:	sense strand is shown >T5493 CCACGCGTCCGCGGTTGATGGTTGACCGATCCTTGGATCCAGGGTAGGGGTCGCTGCGCGTGCGATCGTC TCACACTGGACTACAGGGCGCGGACTGCTGCAGCTCAACCTGGAACCTACCGGCGAGACCAGGCTCCGCC CGGCCATGCCCGCCGGGAGCAACGAGCCGGACGGCGTCCTCAGCTATCAGAGGCCAGATGAGGAAGCTGT GGTGGATCAGGGTGGGACCAGCACAATTCTCAACATTCACTATGAGAAAGAAGAGTTGGAAGGTCATAGA ACTCTGTATGTGGGGGTTCGGATGCCGCTGGGGAGGCAGAGCCATCGGCACCACCGCACTCATGGCCAGA AGCACCGGAGACGAGGAGGGCGGGGCAAAGGAGCCAGCCAGGGCGAGGAGGGCTTGGAAGCCTTGGCCCA CGACACCCCGTCTCAGCGTGTTCAGTTCATTCTTGGCACTGAGGAAGATGAAGAACATGTGCCTCATGAG CTCTTCACAGAGCTGGACGAGATCTGTATGAAAGAGGGGGAAGATGCTGAGTGGAAGGAGACGGCCAGGT GGCTGAAGTTTGAAGAGGATGTGGAGGACGGGGGAGAGCGCTGGAGCAAGCCTTACGTGGCCACGCTCTC TCTGCACAGCCTGTTTGAGCTGAGGAGCTGCCTCATCAACGGCTCTGTGCTTCTGGACATGCGTGCAAGT AGCATAGAGGAGATCTCAGACCTGATCCTGGACCAGCAGGAGCTGCTCCGAGACCTGAGCGACAGCGTGA GGGTTAAAGTGCGGGAAGCCCTGCTGAAGAAGCACCACCACCAGAACGAGAGGAGGAGGAACAACCTCAT CCCCATCGTCCGCTCCTTCGCTGAGGTTGGCAAGAAGCAGTCTGACCCGCACTCCATGGACAGAGATGGG CAAACGGTGTCTCCTCAGTCAGCTACAAACCTTGAAGTGAAGAATGGTGTGAACTGTGAGCACAGCCCTG TGGATCTAAGCAAGGTGGATCTTCACTTCATGAAAAAAATTCCCACGGGGGCAGAGGCCTCCAATGTCCT GGTGGGAGAGGTGGATACGCTGGACCGGCCCATCGTGGCCTTCGTGAGGCTCTCCCCGGCTGTGCTGCTC TCTGGCCTGACGGAAGTGCCCATCCCAACGAGGTTTTTGTTTATCTTACTGGGCCCAGTGGGAAAAGGGC AGCAGTACCATGAGATTGGCAGGTCCATGGCCACCATCATGACGGACGAGATTTTTCATGATGTGGCATA TAAAGCAAAGGAACGCGATGACCTCCTGGCAGGGATTGACGAGTTCCTGGACCAGGTGACGGTGCTTCCG CCAGGGGAGTGGGACCCATCTATTAGAATTGAGCCACCCAAGAATGTCCCTTCCCAGGAAAAAAGGAAAA TGCCCGGTGTTCCAAACGGAAACGTTTGCCACATAGAACCAGAGCCACATGGGGGGCACAGTGGGCCAGA ACTGGAGCGGACTGGGCGGCTGTTTGGGGGCTTGGTGCTGGATGTCAAGCGGAAGGCTCCCTGGTACTGG AGTGACTACCGGGATGCTCTCAGCTTACAGTGTCTGGCCTCCTTCCTGTTCCTGTACTGTGCCTGCATGT CGCCCGTTATCACCTTCGGGGGACTGCTGGGAGAAGCCACTGAAGGACGCATAAGTGCGATTGAGTCGTT GTTTGGAGCGTCCATGACGGGGATTGCCTACTCTCTGTTTGCTGGGCAGCCTCTCACCATCCTGGGAAGC ACGGGGCCCGTGCTGGTGTTTGAAAAGATTTTGTTCAAATTCTGCAAGGACTATGCCCTTTCGTACCTGT CCCTGCGGGCTCTCATCGGGCTGTGGACTGCCTTCCTGTGCATCGTCCTGGTGGCCACGGATGCCAGCTC CCTCGTCTGCTACATTACCCGCTTCACCGAGGAAGCGTTTGCCTCCCTGATTTGTATCATCTTCATCTAT GAAGCCATAGAAAAGCTGATCCACCTGGCAGAGACCTACCCCATCCACATGCACAGCCAGCTGGACCACC TGAGCCTCTATTACTGCAGGTGTGTGCTCCCGGAGAATCCAAACAACCACACCTTACAGTATTGGAAGGA CCACAACATCCTGGCTGCCGAAGTGAACTGGGCAAACCTGACAGTCAGCGAATGCCAGGAGATGCACGGG GAGTTCATGGGATCTGCCTGCGGTCACCACGGACCCTACACGCCCGACGTGCTCTTTTGGTCCTGCATTC TGTTCTTCGCCACCTTCATCGTCTCCAGCACCTTAAAGACATTTAAGACAAGCCGCTACTTCCCGACCAG GGTCCGCTCCATGGTGAGTGACTTCGCCGTGTTCCTCACCATCTTCACCATGGTGGTCCTTGACTTTCTG ATTGGCGTCCCGTCGCCAAAGCTTCAGGTTCCCAATGTGTTCAAGCCAACAAGGGACGATCGAGGATGGT TTATTAATCCCATTGGCCCCAATCCCTGGTGGACTGTGATAGCCGCAATTATCCCAGCTCTCCTGTGCAC TATCCTGATATTCATGGACCAGCAGATCACCGCTGTCATCATTAACAGGAAGGAACACAAGCTCAAGAAA GGCTGTGGCTACCACCTGGACCTGCTCATGGTGGCCGTCATGCTGGGAGTTTGCTCCATCATGGGCCTGC CTTGGTTTGTGGCCGCGACCGTCCTGTCCATTACCCATGTGAACAGCCTCAAGCTGGAATCCGAATGCTC TGCCCCCGGGGAGCAGCCCAAGTTCTTGGGCATCCGGGAACAGAGAGTCACAGGGCTTATGATCTTCGTG CTGATGGGCTGCTCAGTCTTCATGACAGCTGTCTTAAAGTTTATCCCGATGCCAGTGCTCTACGGAGTTT TCCTCTACATGGGAGTCTCTTCGCTACAAGGAATCCAGTTCTTCGACCGTCTGAAGCTTTTCGGGATGCC TGCAAAACACCAGCCAGACTTCATCTACCTGCGGCACGTGCCGCTGCGCAAGGTGCACCTGTTCACGCTA GTGCAGCTCACCTGCCTCGTCCTGCTCTGGGTCATCAAGGCATCGCCGGCCGCCATTGTTTTCCCAATGA TGGTGCTGGCCTTGGTCTTCGTCAGGAAAGTCATGGATCTCTGCTTCTCCAAGCGTGAGCTAAGCTGGCT GGACGACCTCATGCCTGAGAGCAAAAAGAAGAAGCTGGACGATGCGAAGAAGAAGGAGGAGGAGGAGGCC GAGAAGATGTTGGACATTGGGGGTGACAAGTTTCCCCTGGAAAGCAGGAAGCTGCTAAGTAGTCCTGGCA AGAGCAGCAGCTTCAGATGTGACCCTTCTGAGATTAATATATCAGATGAAATGCCTAAAACCACGGTCTG GAAGGCTCTCAGCATAAATTCCGGAAATACAAAAGAAAAAAGCCCCTTCAACTGAGAGTCTCTGCTGAGT GGAAGATTTGGACCATGAGGTGCCTCGGAGAAGTTCTGGGTTTTAACAAAATGGCGAGTCTCTCCGGGAA GAAGCCGAGCTGAGTCTGGCCCCGTTCTTGGTCATCTCAAAGCCATGCCACATGGAAGCATTCAGTCACT CCCGGTGTGCGTTTGGAGGACATCCAGCTACCCACATACCAGCAGCCAGGCGCCAGTCGGGTCATGGTGG GGCCGCCTCCCGCTGCCTCCTTTCCTCTTCCTTCTCTCTCTTCAGAGCTGCCATCATTAAAGAGCCAGCT TCCATTTTCAGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5401143 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5401143 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19340 same embryo
	EMAGE:19337 same embryo
	EMAGE:19339 same embryo
	EMAGE:19338 same embryo
	EurExpress:euxassay_002110 same experiment
	MGI:4828247 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source