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EMAGE:19634

Mum1 melanoma associated antigen (mutated) 1 ( MGI:1915364)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19634 EMAGE:19634 EMAGE:19634 EMAGE:19634 EMAGE:19634
"Pseudo-wholemount" of euxassay_005157. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005157_01 euxassay_005157_02 euxassay_005157_03 euxassay_005157_04
EMAGE:19634 EMAGE:19634 EMAGE:19634 EMAGE:19634 EMAGE:19634
euxassay_005157_05 euxassay_005157_06 euxassay_005157_07 euxassay_005157_08 euxassay_005157_09
EMAGE:19634 EMAGE:19634 EMAGE:19634 EMAGE:19634 EMAGE:19634
euxassay_005157_10 euxassay_005157_11 euxassay_005157_12 euxassay_005157_13 euxassay_005157_14
EMAGE:19634 EMAGE:19634 EMAGE:19634 EMAGE:19634 EMAGE:19634
euxassay_005157_15 euxassay_005157_16 euxassay_005157_17 euxassay_005157_18 euxassay_005157_19
EMAGE:19634
euxassay_005157_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19634Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19634_wholemount_strong.wlz
19634_wholemount_moderate.wlz
19634_wholemount_weak.wlz
19634_wholemount_possible.wlz
19634_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19634_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 05 06 09 10 11 12 13 14 weak expression: see section 01 02 03 04 07 08 15 16 17 18 19
thymus primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11
vibrissa
moderate moderate
regionalmoderate expression: see section 05 06 17 weak expression: see section 07
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20
midbrain mantle layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 moderate expression: see section 04 05
midbrain ventricular layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 moderate expression: see section 04 05 14 15 not examined expression: see section 16
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 05 14
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 14 15 16 weak expression: see section 17 18 19
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 14 15
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 06 11 weak expression: see section 07
cervical ganglion
moderate moderate
regionalmoderate expression: see section 06 07 13
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 07 08 10 11
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 09 10 11 12
retina
moderate moderate
regionalmoderate expression: see section 01
naris
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 14
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 12 13 14 15 16
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14
tongue
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 09 10 12 13
lower jaw molar
moderate moderate
regionalmoderate expression: see section 05 16
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 10 13 14
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 16
metanephros
moderate moderate
regionalmoderate expression: see section 05 06 10 11 12 13 weak expression: see section 02 03 04
lung
moderate moderate
regionalmoderate expression: see section 01 05 06 08 09 10 12 13 weak expression: see section 02 03 04 07 11 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8617
Entity Detected:Mum1, melanoma associated antigen (mutated) 1 ( MGI:1915364)
Sequence:sense strand is shown

>T8617
CCTCTGGCCTCAGTCAAGCCTGTACCACCGCACGCCCAGATACTCCTTTCTGAGTCATGACGGATGCCAA
GTATGTCCTCTGCCGATGGGGGAAGCGACTGTGGCCTGCGAAGGTTTTGGCCAGAACCGAGACTTCAGCA
AAAAACAAGAAAAAGAAGGAATTCTTCCTAGATGTTCAGATACTCTCGCTGAAGGAAAAGATCCAGGTTA
AGAGCTCAGCCGTGGAGGCCCTGCAGAAGTCGCACATTGAGAACATTGCTGCCTTCCTGGCCTCTCAGAA
TGAAGTCCCAGCTACTCCCCTGGAGGAGCTGACTTACCGACGCTCCCTGCGAGTGGCCCTGGATGTCTTG
AATGAGAGGACCAGTTTGAGTCCTGAAAGTCATCCAATAGAAGATGGGATTACACTGTCTCAGAAGGAGA
AAACAGATGCAGATGTGGCCTCGCAGGTCTCCAGTGCTCCTTCTCCATCTCTCCTCGGTGAAGATGGTCA
AGCCGTGGTAGCCCAGTGCGCATCCAAGAGGAGGTCGGAGTACAGTTCAAAAAGCCTGTTGCCGTCGTCT
GCATTAGAAGATCATCTCAGGTGCCAAGTGGGCCCCAAGACAGGCCTCTCAGAGAGTGGAGCTGGAGACA
AGTCTCAGGATGATTCTGGGTTACAGCTGGACCATGGGCAGGAGAGCACAACCAAAAAGAGGCAGAGGAA
TTTGGGAGAGAAACCTACCCGGCGCCGAAGATCAGAGTCTGGCCTTTCCAAGGGAGAAAGTGTCTTAAAG
AGCCAGGGACAGGCATCCAGCTGTGTGGCCCTGGCTTCACCTAGGCCGCCCTCCCAAACCAGGGACGAGG
AGCCCTGTGCCGGGGTCAAAGGCTGTGACTGGGTCAAGTCATCAGGCAACATCAGGCCACTCTCGGCCTC
TGAGAGAAGCAGAGGATGCCCCACAAAGAGACCACGCTTGGATGGAGGCCAGAACCCACCTACCAGGCAG
CTGGGGACTAGAACTGTGGGGGCAGCGCCCTGCCCTAGGAGCTGTTCTGGGGAGGTCACGATGCTGTGCA
GTGCTGGAGCGGGTGACAAACCAGAGGAAGATCCTGTGTCCTCAGAAGAATCCACAGGGTTCAAGTCCAC
ACACTCCCTGCTGGAGGAGGAGGAGGAGGAAGAGGAGGAACCACCTCGGATCCTTCTGTATCACGAACCA
CGGTCATTTGAAGTAGGAATGCTGGTCTGGCTTAAATACCAAAAATACCCATTCTGGCCAGCTGTGGTCA
AGAGTGTCCGGCGAAGAGACAAGAAGGCCAGTGTGCTCTTCATTGAGGGCAACATGAACCCCAAGGGCCG
AGGAATCACTGTGTCGCTGCGACGGCTCAAGCACTTTGACTGCAAGGAAAAGCACGCACTGCTGGACAGA
GCCAAGGAGGACTTTGCCCAGGCCATTGGCTGGTGTGTTTCACTCATCACTGACTACCGTGTGCGGCTGG
GATGCGGCTCCTTTGCTGGGTCTTTCTTGGAATACTACGCTGCTGATATCAGCTACCCTGTGCGCAAGTC
TATCCAACAGGACGTCCTGGGGACCAGGTTTCCTCAGCTGGGCAAGGGGGACCCTGAGGAGCCTGTAGGG
GACAGCCAGCTGGGACAGTGGCGGCCATGCAGGAAGGTGCTGCCTGACCGCTCCAGGGCTGCACGGGATA
GAGCCAACCAGAAGCTGGTGGAGTACATCGTGAAGGCCAAGGGTGCAGAGAGCCACCTGCGGGCTATCTT
GCACAGCCGCAAGCCCTCACGCTGGCTGAAGACGTTCCTGAGCTCCAGTCAGTGCGTGACTTGCATGGAG
ACATACCTGGAGGATGAGGCGCAGCTGGATGAGGTGGTGGAGTACCTGCAGGGCGTCTGCAGAGACATGG
ACGGCCAGGTGCCTGAGCGCGGCAGTGGAGACCGCATTCGCTTCATCTTGGATGTGCTGCTGCCCGAGGC
AATCATCTGTGCCATTTCCGCGGTGGAGGCGGTGGATTACAAGACAGCCGAGCAGAAGTACATTCGTGGC
CCCACACTTAGCTACCGGGAAAAGGAAATCTTTGACAATGAACTCCTGGAGGAGAGGAACCGACGCCGTC
GCTGATGCCGCAGTCTGCACCTGGCCGGCCAGCACTGGGTGTGACCTGCCAGAGGCCTCCACCAGGCCTA
GGAGTGTGAGAACTTTGACCTGTGCGTGGGTTCTACGTCTTCAGCCTAAGTCTGGGAGATCAGATGCCAT
CAGATGCCTGCTGCCATCTATGAACAGCTGCTTTCTGGAAGTTTCTGTGTGTTTTTGTGTATACATGTGT
ATGTCTGGTTTTATTTTTTTTAATTATTTTGTTTATAAATGCGTTTGAATGCAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5718202 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5718202 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19638 same embryo
 EMAGE:19635 same embryo
 EMAGE:19636 same embryo
 EMAGE:19639 same embryo
 EMAGE:19637 same embryo
 EurExpress:euxassay_005157 same experiment
 MGI:4826509 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS