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EMAGE:7007

Egfr epidermal growth factor receptor ( MGI:95294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007
"Pseudo-wholemount" of euxassay_002564. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002564_01 euxassay_002564_02 euxassay_002564_03 euxassay_002564_04
EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007
euxassay_002564_05 euxassay_002564_06 euxassay_002564_07 euxassay_002564_08 euxassay_002564_09
EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007
euxassay_002564_10 euxassay_002564_11 euxassay_002564_12 euxassay_002564_13 euxassay_002564_14
EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007
euxassay_002564_15 euxassay_002564_16 euxassay_002564_17 euxassay_002564_18 euxassay_002564_19
EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007 EMAGE:7007
euxassay_002564_20 euxassay_002564_21 euxassay_002564_22 euxassay_002564_23 euxassay_002564_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7007Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7007_wholemount_strong.wlz
7007_wholemount_moderate.wlz
7007_wholemount_weak.wlz
7007_wholemount_possible.wlz
7007_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7007_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
diaphragm
strong strong
regionalstrong expression: see section 13 14 15 16 17 moderate expression: see section 09 10 11 12 18 weak expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 23 24
elbow mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03
forearm mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03
shoulder mesenchyme
moderate moderate
regionalmoderate expression: see section 02 03
upper arm
moderate moderate
regionalmoderate expression: see section 01
upper arm mesenchyme
moderate moderate
regionalmoderate expression: see section 02 03 04
hand mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05 06 07
hindlimb digit 1 phalanx
moderate moderate
regionalmoderate expression: see section 07 08 09 18 19 20
hindlimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 07 08 09 18 19 20
hindlimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 07 08 09 18 19 20
hindlimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 07 08 09 18 19 20
hindlimb digit 5 phalanx
moderate moderate
regionalmoderate expression: see section 09 18 19 20
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 18 19 20 21 22 23
hip mesenchyme
moderate moderate
regionalmoderate expression: see section 02 03 04 24
knee mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 24
lower leg mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 24
upper leg mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 21 22 23 24
epidermis
strong strong
regionalstrong expression: see section 05 06 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 01 02 03 04 07 22 23 24
vibrissa
strong strong
regionalstrong expression: see section 05 06 07 19 20 21 moderate expression: see section 04 08 09 22 23
hypothalamus mantle layer
strong strong
single cellstrong expression: see section 08 09 10 11 12 13 15 16 17 18 19
hypothalamus ventricular layer
strong strong
single cellstrong expression: see section 13 15
diencephalon lateral wall mantle layer
strong strong
single cellstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
diencephalon lateral wall ventricular layer
strong strong
single cellstrong expression: see section 13 14 15
telencephalon mantle layer
strong strong
single cellstrong expression: see section 05 06 12 13 14 15 16 17 18 19 moderate expression: see section 02 03 04 07 08 11 20 21 22 23 24
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 04 05 06 11 12 16 17 21 22 23 24 weak expression: see section 07 08 19 20
medulla oblongata basal plate mantle layer
strong strong
single cellstrong expression: see section 15 16 17 18 19 20 moderate expression: see section 09 10 11 12 13 14
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 13 15
rest of cerebellum mantle layer
strong strong
single cellstrong expression: see section 19 20 23 moderate expression: see section 06 07 08
pons mantle layer
strong strong
single cellstrong expression: see section 15 16 17 18 19 20 21 22 moderate expression: see section 07 08 09 10 11 12 13 14
pons ventricular layer
strong strong
regionalstrong expression: see section 13 15 16 17 18 19 moderate expression: see section 11 12 14
midbrain mantle layer
strong strong
single cellstrong expression: see section 15 16 17 18 19 20 21 moderate expression: see section 08 09 10 11 12 13 14
midbrain ventricular layer
strong strong
regionalstrong expression: see section 13 15 16 17 18 moderate expression: see section 11 12
intermediate grey horn
strong strong
single cellstrong expression: see section 12 13 14 15 16 17
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 04 24
nasal septum
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
mandible
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24
maxilla
moderate moderate
regionalmoderate expression: see section 07 08 09 11 12 15 16 17 19 20
palatal shelf mesenchyme
moderate moderate
regionalmoderate expression: see section 12 16 17 19 20
primary palate
moderate moderate
regionalmoderate expression: see section 10 11 12 15
trachea
moderate moderate
regionalmoderate expression: see section 14 15
axial skeleton cervical region
moderate moderate
regionalmoderate expression: see section 10 11 12 13 16 17
axial skeleton lumbar region
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17
axial skeleton sacral region
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15
axial skeleton thoracic region
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 16 17
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 11 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5185
Entity Detected:Egfr, epidermal growth factor receptor ( MGI:95294)
Sequence:sense strand is shown

>T5185
CCACGCGTCCGGCCCCAGCGCAACGCGCAGCAGCCTCCCTCCTCTTCTTCCCGCACTGTGCGCTCCTCCT
GGGCTAGGGCGTCTGGATCGAGTCCCGGAGGCTACCGCCTCCCAGACAGACGACAGGTCACCTGGACGCG
AGCCTGTGTCCGGGTCTCGTCGTTGCCGGCGCAGTCACTGGGCACAACCGTGGGACTCCGTCTGTCTCGG
ATTAATCCCGGAGAGCCAGAGCCAACCTCTCCCGGTCAGAGATGCGACCCTCAGGGACCGCGAGAACCAC
ACTGCTGGTGTTGCTGACCGCGCTCTGCGCCGCAGGTGGGGCGTTGGAGGAAAAGAAAGTCTGCCAAGGC
ACAAGTAACAGGCTCACCCAACTGGGCACTTTTGAAGACCACTTTCTGAGCCTGCAGAGGATGTACAACA
ACTGTGAAGTGGTCCTTGGGAACTTGGAAATTACCTATGTGCAAAGGAATTACGACCTTTCCTTCTTAAA
GACCATCCAGGAGGTGGCCGGCTATGTCCTCATTGCCCTCAACACCGTGGAGAGAATCCCTTTGGAGAAC
CTGCAGATCATCAGGGGAAATGCTCTTTATGAAAACACCTATGCCTTAGCCATCCTGTCCAACTATGGGA
CAAACAGAACTGGGCTTAGGGAACTGCCCATGCGGAACTTACAGGAAATCCTGATTGGTGCTGTGCGATT
CAGCAACAACCCCATCCTCTGCAATATGGATACTATCCAGTGGAGGGACATCGTCCAAAACGTCTTTATG
AGCAACATGTCAATGGACTTACAGAGCCATCCGAGCAGTTGCCCCAAATGTGATCCAAGCTGTCCCAATG
GAAGCTGCTGGGGAGGAGGAGAGGAGAACTGCCAGAAATTGACCAAAATCATCTGTGCCCAGCAATGTTC
CCATCGCTGTCGTGGCAGGTCCCCCAGTGACTGCTGCCACAACCAATGTGCTGCGGGGTGTACAGGGCCC
CGAGAGAGTGACTGTCTGGTCTGCCAAAAGTTCCAAGATGAGGCCACATGCAAAGACACCTGCCCACCAC
TCATGCTGTACAACCCCACCACCTATCAGATGGATGTCAACCCTGAAGGGAAGTACAGCTTTGGTGCCAC
CTGTGTGAAGAAGTGCCCCCGAAACTACGTGGTGACAGATCATGGCTCATGTGTCCGAGCCTGTGGGCCT
GACTACTACGAAGTGGAAGAAGATGGCATCCGCAAGTGTAAAAAATGTGATGGGCCCTGTCGCAAAGTTT
GTAATGGCATAGGCATTGGTGAATTTAAAGACACACTCTCCATAAATGCTACAAACATCAAACACTTCAA
ATACTGCACTGCCATCAGCGGGGACCTTCACATCCTGCCAGTGGCCTTTAAGGGGGATTCTTTCACGCGC
ACTCCTCCTCTAGACCCACGAGAACTAGAAATTCTAAAAACCGTAAAGGAAATAACAGGCTTTTTGCTGA
TTCAGGCTTGGCCTGATAACTGGACTGACCTCCATGCTTTCGAGAACCTAGAAATAATACGTGGCAGAAC
AAAGCAACATGGTCAGTTTTCTTTGGCGGTCGTTGGCCTGAACATCACATCACTGGGGCTGCGTTCCCTC
AAGGAGATCAGTGATGGGGATGTGATCATTTCTGGAAACCGAAATTTGTGCTACGCAAACACAATAAACT
GGAAAAAACTCTTCGGGACACCCAATCAGAAAACCAAAATCATGAACAACAGAGCTGAGAAAGACTGCAA
GGCCGTGAACCACGTCTGCAATCCTTTATGCTCCTCGGAAGGCTGCTGGGGCCCTGAGCCCAGGGACTGT
GTCTCCTGCCAGAATGTGAGCAGAGGCAGGGAGTGCGTGGAGAAATGCAACATCCTGGAGGGGGAACCAA
GGGAGTTTGTGGAAAATTCTGAATGCATCCAGTGCCATCCAGAATGTCTGCCCCAGGCCATGAACATCAC
CTGTACAGGCAGGGGACCAGACAACTGCATCCAGTGTGCCCACTACATTGATGGCCCACACTGTGTCAAG
ACCTGCCCAGCTGGCATCATGGGAGAGAACAACACTCTGGTCTGGAAGTATGCAGATGCCAATAATGTCT
GCCACCTATGCCACGCCAACTGTACCTATGGATGTGCTGGGCCAGGTCTTCAAGGATGTGAAGTGTGGCC
ATCTGGGTACGTTCAATGGCAGTGGATCTTAAAGACCTTTTGGATCTAAGACCAGAAGCCATCTCTGACT
CCCCTCTCACCTTCCAGTTTCTTCCAAATCCTCTGGGCCAGCCAGAGGTCTCAGATTCTGCCCTCTTGCC
CTGTGCCCACCTTGTTGACCACTGGACAGCATATGTGATGGCTACTGCTAGTGCCAGCTTCACAAGAGGT
TAACACTACGGACTAGCCATTCTTCCTATGTATCTGTTTCTGCAAATACAGCCGCTTTACTTAAGTCTCA
GCACTTCTTAGTCTCCTCTTTTCCTCTCAGTAGCCCAAGGGGTCATGTCACAAACATGGTGTGAAGGGCT
ACTTTGTCAAATGAAAAGGTCTATCTTGGGGGGCATTTTTTTCTTTTCTTTTTTTCTTGAAACACATTGC
CCAGCAAAGCCAATAAATTTCTCTCATCATTTTGTTTCTGATAAATTCTTACTATTGATAAAAAAAAAAA
AAAAAA
Notes:The probe template was PCR amplified from IMAGE:5351807 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5351807 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7010 same embryo
 EMAGE:7006 same embryo
 EMAGE:7009 same embryo
 EMAGE:7008 same embryo
 EMAGE:7011 same embryo
 EurExpress:euxassay_002564 same experiment
 MGI:4824492 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS