EMAGE mouse gene expression spatial database

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EMAGE:7104

Krt7 keratin 7 ( MGI:96704)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002600. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002600_01	euxassay_002600_02	euxassay_002600_03	euxassay_002600_04

euxassay_002600_05	euxassay_002600_06	euxassay_002600_07	euxassay_002600_08	euxassay_002600_09

euxassay_002600_10	euxassay_002600_11	euxassay_002600_12	euxassay_002600_13	euxassay_002600_14

euxassay_002600_15	euxassay_002600_16	euxassay_002600_17	euxassay_002600_18	euxassay_002600_19

euxassay_002600_20	euxassay_002600_21	euxassay_002600_22	euxassay_002600_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7104_wholemount_strong.wlz

7104_wholemount_moderate.wlz

7104_wholemount_weak.wlz

7104_wholemount_possible.wlz

7104_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7104_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 14 15 weak expression: see section 13

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 17 18 19

foregut-midgut junction

moderate

regional

moderate expression: see section 12 13 14

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23

naris

strong

regional

strong expression: see section 10 11 12 13 14 15 16

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 14 15 moderate expression: see section 08 09 10 16 17 weak expression: see section 18

esophagus

strong

regional

strong expression: see section 12 13

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 moderate expression: see section 10

hindgut

strong

regional

strong expression: see section 08 moderate expression: see section 11 12 13 14 15 16 17 18

rectum

strong

regional

strong expression: see section 13

midgut

strong

regional

strong expression: see section 05 06 07 09 moderate expression: see section 10 11 12 13 14 15 16 17 18 19

lower jaw incisor

strong

regional

strong expression: see section 10 11 13 14 15 16

lower jaw molar

strong

regional

strong expression: see section 08 18

oral epithelium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

upper jaw incisor

strong

regional

strong expression: see section 11 14 15

upper jaw molar

strong

regional

strong expression: see section 07 18

bladder

strong

regional

strong expression: see section 12 13 14

kidney calyx

strong

regional

strong expression: see section 08 09 10 17 18 19

urethra of male

strong

regional

strong expression: see section 12 13 14

lung

strong

spotted

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

trachea

strong

regional

strong expression: see section 12 13 14 15

nucleus pulposus

strong

regional

strong expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5221
Entity Detected:	Krt7, keratin 7 ( MGI:96704)
Sequence:	sense strand is shown >T5221 CCACGCGTCCGGTCGCAGACTGCCTGAGGTCAGGGAGTTCGCGCTGCTCCTCGCCTCGTCCAGCCAGCTG TGTCCAGCCGCTATGTCCATCCACTTCAGCTCTCGCTCCACTGCTTACCCGGGCCGCGGGGCTCAGGTGC GCCTGAGCTCGGGCCGCGCCAGCTTCGGCAGCAGGAGCCTCTATGGCCTGGGCTCATCAAGGCCTCGTGT GGCCGTGCGCTCCGCTTATGGGGGCCCGGTGGGCGCTGGCATCCGCGAGATCACCATCAATCAGAGCTTG CTGGCGCCGCTGAGTGTGGACATCGATCCCACCATCCAGCAGGTGCGCCAGGAGGAGCGCGAGCAGATCA AGACCCTCAACAACAAATTCGCCTCCTTCATCGACAAGGTACGCTTCCTGGAACAGCAGAACAAGATGCT GGAGACCAAGTGGGCGCTGCTGCAGGAACAGAAGTCAGCCAAGAGCAGCCAGCTTCCCCGAATCTTTGAG GCTCAGATTGCTGGGCTGAGGCAGCAGCTCGAGACACTGCAGCTGGATGGGGGCCGCCTGGAGGTGGAAC TGCGGAACATGCAGGATGTGGTGGAAGATTTCAAGAATAAGTATGAAGAGGAGATCAACCGACGCACGGC TGCTGAGAATGAGTTTGTGTTGCTGAAGAAGGATGTGGATGCCGCCTACACGAACAAGGTGGAGTTGGAG GCCAAGGCGGACAGTCTGCAGGATGAGATCAACTTCCTCAAGACCCTTCACGAGACAGAGTTAGCAGAGC TGCAGTCGCAGATCTCAGACACATCTGTGGTGCTGTCCATGGACAACAGCCGCTCCCTGGACTTGGATGG TATCATTGCTGACGTCAAAGCCCAGTATGAGGAGATGGCCAACCACAGCCGGGCAGAGGCTGAAGCTTGG TACCAGACCAAGTTTGAGACACTCCAGGCCCAGGCTGGGAAGCACGGGGATGACCTCCGCAACACCCGGA ATGAGATTGCGGAGATGAACCGCTCTATCCAGAGGCTGCAGGCAGAGATTGACACCTTGAAGAACCAGCG TGCCAAGTTAGAGTCCAGCATCGCAGAGGCTGAGGAACAAGGGGAGCTGGCAATCAAGGATGCTCATGCT AAGCAGGGGGAGCTGGAGGCTGCCCTGCAGAAGGCCAAGCAGGATGTGGCCCGGCAACTTCGAGAGTACC AGGAGCTCCTGAACACCAAGCTGGCGCTGGACATTGAGATCGCCACCTACCGCAAGCTGCTGGAGGGCGA GGAGAGCCGGTTGTCTGGAGACGGAATGGGACCTGTGAATATCTCTGTTGTGAATTCCACTGGGGGCAAT GGTGGCAAGCTTATCTTCGGAGGAACCATGGGAAGCAATGCTCTGAGCTTCAGTGGGGGACCCGGAGCCC TCAGGGCCTATTCCATCAAGACCACATCTACTACCCGCAGGGGCACCCACAATTGAGCACATACAACCAA GCTGTGGGCTTGGAGAGACCCCCCATACCCTTTTGTCCATCTCATGTCCCTGTCCCGAGCCTGGAAGATA GTCTGAAACTGCTGTTGGCTTTAATAAAGGGCCTCTCTGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5355662 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5355662 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7107 same embryo
	EMAGE:7108 same embryo
	EMAGE:7105 same embryo
	EMAGE:7103 same embryo
	EMAGE:7106 same embryo
	EurExpress:euxassay_002600 same experiment
	MGI:4825842 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source