EMAGE mouse gene expression spatial database

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EMAGE:7268

Cdcp1 CUB domain containing protein 1 ( MGI:2442010)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004357. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004357_01	euxassay_004357_02	euxassay_004357_03	euxassay_004357_04

euxassay_004357_05	euxassay_004357_06	euxassay_004357_07	euxassay_004357_08	euxassay_004357_09

euxassay_004357_10	euxassay_004357_11	euxassay_004357_12	euxassay_004357_13	euxassay_004357_14

euxassay_004357_15	euxassay_004357_16	euxassay_004357_17	euxassay_004357_18	euxassay_004357_19

euxassay_004357_20	euxassay_004357_21	euxassay_004357_22	euxassay_004357_23	euxassay_004357_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7268_wholemount_strong.wlz

7268_wholemount_moderate.wlz

7268_wholemount_weak.wlz

7268_wholemount_possible.wlz

7268_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7268_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14 15 16

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 18 19 20 21 22

adenohypophysis

weak

regional

weak expression: see section 11 12 13 14 15 16

vibrissa

weak

regional

weak expression: see section 04 05 06 07 08 21 22 23 24

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 04 05 06 24

cornea epithelium

moderate

regional

moderate expression: see section 04 05 24 weak expression: see section 02 03

naris

moderate

regional

moderate expression: see section 13 14 15 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 16 17 weak expression: see section 18 19 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 16 17

esophagus

weak

regional

weak expression: see section 13 14

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 14

rectum

moderate

regional

moderate expression: see section 14 15

midgut

moderate

regional

moderate expression: see section 12 13 18

midgut loop

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

weak

regional

weak expression: see section 11 12 13 16 17

lower jaw molar

weak

regional

weak expression: see section 08 09 20

oral epithelium

moderate

regional

moderate expression: see section 21 22 weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

upper jaw incisor

weak

regional

weak expression: see section 11 12 13 16 17

upper jaw molar

weak

regional

weak expression: see section 08 09 20

bladder

weak

regional

Expression in the fundus region.

left lung

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

right lung

weak

regional

weak expression: see section 15 16 17 18 19 20 21 22 23 24

trachea

weak

regional

weak expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5172
Entity Detected:	Cdcp1, CUB domain containing protein 1 ( MGI:2442010)
Sequence:	sense strand is shown >T5172 GTCCTGCGCGCAGGTGAGCGGCGGGGCAGGGCGGGGCGCAGCTGCGTTTCGGTGCCTAGGCGCCACCGAG GCCACTCGCAGACACAGGCCACAGTCGTGCCCTAGGGAGACCGAGGCGCCCCCCGAGTCATGGCCCACTC GGCCTGCGGGTTCTCAGTCGCACTGCTGGGGGCCCTGCTGCTGGGCACAGCACGCCTGCTGCGCGGGACA GAAGCATCTGAGATTGCTCTGCCCCAGAGAAGTGGCGTCACAGTGAGCATCAAGCTGGGGAACCCAGCCC TTCCTGTGAAAATCTGCTACATCGTCATGTCTAGACAGCATATAACCGAGTTGATTATCAGACCTGGAGA AAGAAAGTCTTTTACCTTTAGCTGCAGCAATCCAGAGAAGCACTTTGTCTTGAAGATCGAGAAGAATATT GACTGCATGTCAGGCCCGTGTCCTTTTGGGGAGGTTCATCTTCAGCCATCCACATCAGAGCTACCCATCC TCAACAGAACCTTCATCTGGGATGTTAGAGCTCATAAGAGCATTGGCTTGGAGCTGCAGTTCGCAACCCC TCGACTGAGGCAGATTGGACCAGGCGAGAGCTGTGCAGACGGGGTGACTCACTCCATCAGTGGCCATATA GATGCCACTGAGGTCAGAATTGGGACCTTCTGCAGTAACGGCACCGTGTCCCGGATCAAGATGCAAGAGG GTGTGAAAATGGCCTTACACCTGCCCTGGTTCCACCGCAGGAACGTCTCAGGCTTCAGTATTGCAAATCG GTCATCTATCAAACGCCTGTGCATCATCGAGTCAGTATTTGAGGGTGAAGGCTCAGCAACTCTGATGTCA GCCAACTACCCAGGAGGCTTCCCTGAAGATGAGCTTATGACCTGGCAGTTTGTTGTCCCCGCACACCTAA GGGCCAGTGTCTCCTTCCTCAACTTCAATGTCTCCAACTGTGAGAGGAAGGAGGAACGCGTTGAATACTA TATCCCAGGCTCCACCACCAACCCAGAGGTGTTCAGGCTGGAGGACAAGCAGCCAGGAAACATGGCTGGG AACTTCAACCTCTCCCTGCAAGGCTGCGACCAGGATGCCCAGAGCCCAGGAATCCTTCGGCTTCAGTTCC AGGTCCTGGTCCAACGTCCACAGGATGAAAGCAATAAAACCTACATGGTGGACCTGAGTCGTGAGCGAAC CATGTCGCTCACCATAGAGCCACGGCCAGTAAAACATGGCCGCAGGTTTGTTCCTGGGTGCTTCGTGTGT CTAGAGTCTCGGACTTGTAGTACCAATGTCACCCTGACAGCTGGCTCCATACACAAGATTTCCTTCCTGT GCGATGACTTGACACGCCTGTGGGTGAATGTGGAGAAAACCCTAAGTTGCCTGGACCACCGCTACTGCTA TAGGCAGTCCTTCAAACTGCAGGTGCCTGACTATATCCTCCAGCTGCCTGTACAGCTGCATGACTTCTCC TGGAAGCTACTGGTGCCCAAGGACAAGCTCAGCCTTATGCTGGTGCCAGGCCAGAAGCTGCAGCAACATA CGCAAGAGAGGCCTTGTAACACCAGCTTCGGGTACCACGTGACCAGCACCACCCCTGGCCAGGACCTGTA CTTTGGTTCCTTCTGCTCAGGAGGCTCCATCGAGAAGATCCAGGTGAAGCAAAATAGCTCGGTGACACTG AGAGCCTATGCTCCCAGCTTTCAACAAGAAGTCTCCAAGCAAGGCCTGATAGTGTCCTATACACCGTATT TCAAAGAGGAAGGCATCTTCACGGTGACCCCAGACACAAAAAACAAGGTCTACCTAAGATCACCCAACTG GGACCGTGGCCTGCCTGCCCTCTCCTCAGTGTCTTGGAACATCAGTGTGCCTAGCAACCAAGTGGCTTGT CTGACCGTCTTGAAAGAGCGTTCTGGCTTGGCCTGCCAGTCAGGGCGTGCATTCATGATCATCCAGGAGC AGCAGTCCCGGGCAGAGGAGATCTTCAGCTTGGAGGAGGAAGTCCTGCCTAAGCCAAGTTTCCACCATCA CAGCTTCTGGGTTAACATCTCCAATTGCAGCCCCATGAATGGCAAGCAGCTAGATTTGCTCTTCTGGGTG ACTCTTACCCCGAGGACTGTAGATTTGGCTGTCGTCATTGGTGCAGCAGGAGGTGGAGCTCTGCTGCTGT TTGCCCTGGTACTCATCATCTGCTTCGTGAAAAAGAAGAAGAAGGTGGATAAGGGTCCTGCTGTGGGTAT CTATAATGGCAATGTCAATACCCAGATGCCCCAGACCCAAAAGTTCCCGAAAGGGCGAAAAGACAATGAC TCCCATGTGTATGCTGTCATCGAAGACACCATGGTGTATGGGCACTTGCTGCAGGACTCGGGTGGATCCT TCATCCAACCAGAGGTGGACACCTACCGGCCCTTCCAGGGCCCCATGGGAGACTGCCCTCCCACCCCACC CCCTCTATTCTCCAGGACCCCAACTGCAAAGTTCACTGCAGAGGAGCTGGCTCCTAGTAGCCCTCCTGAG TCTGAGAGTGAACCTTACACTTTCTCGCATCCCAACAAGGGGGAGATTGGTGTTAGGGAGACTGACATCC CCTTACTCCATACCCAAGGACCAGTGGAGACTGAAGAGTAACTTGGCCCCATTCCAAAGACTTTGCTGAG ATGCATGGCACCAGACATTGAAACATGAAGAACTGTCCCATCAAGAAGTCAGAGGGAAGCCCAATCAGCT AGAAGGAAGAGTAGCTTTTGCAAGGTGCCATCAAATCTTGATCTTCTGGTGGACTGCTTCCAGTGATGGG CAAATCATGACTTCTATGAGGATGCCATCCTAAAGCTCAGGCCTTCCTGAACTCGGGTTGTGTTGAGGCT GGCCACAGGGACAGAAGAGAGACACGGTCCACCCAGACAAGACTACAATGAGCCCTGGAGTTCTGGATTC CCGATGACGAGCAGAGACTTGGATGATTCCCAGGAGCTCCACTTGAGGTCTCTGCTCTCACTGGAGACCT ATGGATGAAAATGGCAATGTGCTTTTTTTAAAATTAGTTGTAGTAGTAGTAGTAGTAGTAGTATTTATTT GGTGGCCCTGTATGTTTTTAAAAAGTTGGATGTGGAGCTGAGGGTGTAGCCAAGTTGGTAGAATGCTTGC CCAACGTGTGCAAAGCTCTGGGCTCCATCCTGCATAAAAGCAGGCAGGATGGTGCACACCTGTAGTCTCG GGAAGATCAGAATTTCAAGTCACCATCAAGTATGCAGTGAATTGAAGGCCCCCTGGTCTGTGTAAGGTCC TCTTGAGAACACGCACAGCTCTTTTGACCCCTGTGGTAACGGCTCGTGCTAACTGGTTTGGACAGCTGTG CTTTGGTTTTTGTAGACCTCTAGATAAATGTACACCTGCGGGGAAGGTGAGAACCCAGCCAGAAGAGTAT CTGCATGTATGTATCTCTTACTCAATAAGGGGTTTCCCAATCCTCCTTGACTGCCTATAGCTGTCCCACT GCAGAATTTATCACAGATCTCCTCGTCCCCTACTCCTAAAAATAAGAGAAAGTTCCTTGAGCTGCCTTGT GTGGTATGTGGCTCTCCCACCTGCAGCAATACTTTGACATGTAAACAGAAAATTGAGAATAACTTGATCC CTTAAAAATATATAAATATAGGCAGTGTTGGTACATGCCTTTAATTCCAGAACTTGGGAGACAGAGGCAG GCATATCCTGGTGAGTTTGAGGCCAATCTGGACTATATAGTGAGTTCTAGGACAGCCAGGGCTACACACA GAAATCCAGCTACACAGAGAAACCCTTTCTTGATAAAACAAAACAAAACAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5347122 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5347122 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7267 same embryo
	EMAGE:7263 same embryo
	EMAGE:7266 same embryo
	EMAGE:7265 same embryo
	EMAGE:7264 same embryo
	EurExpress:euxassay_004357 same experiment
	MGI:4823760 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source