EMAGE mouse gene expression spatial database

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EMAGE:7302

Dgka diacylglycerol kinase, alpha ( MGI:102952)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004404. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004404_01	euxassay_004404_02	euxassay_004404_03	euxassay_004404_04

euxassay_004404_05	euxassay_004404_06	euxassay_004404_07	euxassay_004404_08	euxassay_004404_09

euxassay_004404_10	euxassay_004404_11	euxassay_004404_12	euxassay_004404_13	euxassay_004404_14

euxassay_004404_15	euxassay_004404_16	euxassay_004404_17	euxassay_004404_18	euxassay_004404_19

euxassay_004404_20	euxassay_004404_21	euxassay_004404_22	euxassay_004404_23	euxassay_004404_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7302_wholemount_strong.wlz

7302_wholemount_moderate.wlz

7302_wholemount_weak.wlz

7302_wholemount_possible.wlz

7302_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7302_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

thyroid gland

moderate

regional

moderate expression: see section 11 13

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 09 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 09 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 12 13 14 15 16 17 18

ear

moderate

regional

moderate expression: see section 01

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 23 24

naris

moderate

regional

moderate expression: see section 12 13 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 15 16 17 18 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 14 16 17 18

pharynx

moderate

regional

moderate expression: see section 12 13 14 15

esophagus

moderate

regional

moderate expression: see section 12

tongue epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

stomach

moderate

regional

moderate expression: see section 01 02 03

oral epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

trachea

moderate

regional

moderate expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6427
Entity Detected:	Dgka, diacylglycerol kinase, alpha ( MGI:102952)
Sequence:	sense strand is shown >T6427 GTCCCCTCCTGTCCAGCTCGGGCTCCGGCGCCCCTGCTCTGACCGTGCGCTCTCTTGGGTCAAGGTAGAG CCCCCGCGAGCCCCGTGCCACTCGCACACTCTCGCACCCGGCCAAAAGGGCAGCGGAAGGGGCCGCCCCA CCGACTGGGTGAGGGCTGCGGCGTCCAGAGAAGGAAGCGTTGACAGCTGGAAGCTGCTGCTGGAAGGGTT TGCGCTGGGACTCCGGGACTGCCGTAGCGCTGCGCAAGACGCAAGACCGGGGCTAACCCTCTGAAAAACA AGAAGCTGTACTACCAGGCTGTCTCTGTGATCAACTTCAGGAAGAGAAGAAGGATGGCCAAAGAGAAGGG CCTCATCAGCCCAGAAGACTTCGCTCAGCTGCAAAAGTACATAGAATACTCCACCAAAAGGGTCAGCGAT GTACTGAAGGTCTTTGACGATGGTGAGATGAACAGATTTTGCCAGGGAGATGCCATTGGGTACCTAGGAT TTGAACAATTCATGAAAATGTATCTGGAAATGGAGGAGGTTCCCCATCACCTATGCTGGGCTCTGTTTTG GTCCTTCCATACTAGTCAAGTTGCGGCTGAGAAGACCAAGTCAAAAGCCAATGTGATCTGTCTCAGTGAT GTGTACTGCTACTTCACCCTCCTAGAAGGTGGCCGGCCAGAAGACAAGCTAGAGTTCACCTTCAAGCTAT ATGACATGGACAGAAATGGGATCCTGGATAGCACGGAAGTGGAAAAAATCATCCTTCAGATGATGCGAGT GGCCGAATATCTAGACTGGGATGTGTCTGAGCTGAGACCGATCCTTCAGGAGATGATGAGAGAGATGGAT CAGGACGGCAGTGGCTCTGTGTCTCTAGACGAGTGGGTCCGTGCTGGGGCTACCACAGTGCCACTGCTTG TGCTCCTGGGGATGGACGTGACTATGAAAGATGATGGCAACCATATATGGAGACCCAAGAGATTCACCAG ACTGGTCTACTGCAACCTGTGCGAACAGAGCATTAGCCTTGGCAAACAGGGCCTGAGCTGTAACTTCTGT AAGTACATTGTTCATGACCACTGTGCCATGAAGGCCCAGCCTTGTGAAGTCAGCACCTATGCCAAGTCTC GGAAAGACATTGGTGTCCAGTCACACTTATGGGTTCGAGGAGGCTGTCATTCCGGGCGTTGTGACCGCTG CCAGAAAAAGATCCGGACCTACCACAGCCTAACAGGACTGCACTGTGTGTGGTGCCACCTGGAGATCCAT GATGACTGTCTGCAGGCTGTAGGTCCTGAGTGTGACTGTGGGTTGCTCCGTGATCATATCCTGCCTCCGT GTTCCATCTACCCCAGCGTCCTGGTATCTGGACAAGAGTGCAAACACAAGACCACAGATGATACAAGCCT GTGCACCCCTGAGGCTTTTCGGATTGAACCCGTTTCTAACACCCACCCCCTTCTAGTCTTCATCAATCTT AAGAGCGGAGGCAAGCAGGGGCAGAGTGTGCTTTGGAAATTTCAGTACATTCTGAACCCGCGGCAGGTGT TCGACCTGAAGGATGGTCCGGAGCCAGGGCTCAGATTCTTTAAAGATGTTCCTCAGTTCCGGATATTGGT GTGTGGTGGAGATGGCACAGTAGGCTGGGTTCTAGAGACCATTGACAAAGCCAACTTTGCCACTGTGCCT CCCGTTGCTGTGCTGCCCCTGGGCACTGGAAATGATCTGGCTCGGTGCCTAAGATGGGGAAGAGGTTATG AAGGTGAGAATTTGAGAAAGATTCTCAAGGATATAGAGCTAAGTAAGGTGGTATATCTCGACCGATGGTT CCTGGAAGTGATTCCCCAACAAAATGGAGAAAAGAGTGATCCAGTTCCCTCTCAAATCATCAATAACTAC TTCTCCATTGGCGTGGATGCTTCTATCGCTCACCGATTCCATCTCATGAGAGAGAAATATCCTGAGAAGT TCAATAGCAGAATGAAGAACAAGCTTTGGTACTTTGAGTTTGCCACATCTGAGTCCATCTTCTCAACATG CAAAAAGCTGGAAGAGTCTGTAACCGTTGAGATATGCGGGAAGCTGCTGGATCTGAGCGACCTATCCCTA GAAGGCATTGCGGTATTGAACATCCCGAGCACGCACGGTGGCTCCAACCTCTGGGGTGACACCAAGAGAC CTCATGGGGATACATGTGAGATCAACCAGGCATTGGGCAGTGCGGCCAAAATAATCACAGACCCCGATAT CCTGAAGACCTGTGTGCCAGACATGAGTGACAAGCGGCTGGAAGTGGTAGGAATAGAGGGTGCAATTGAG ATGGGCCAGATCTATACCAGGCTCAAGAGTGCTGGACACCGGCTGGCCAAGTGCTCCGAGATCACATTCC AGACCACAAAAACCCTCCCCATGCAAATTGACGGGGAGCCCTGGATGCAGGCACCCTGTACAATCAAGAT CACCCATAAGAACCAGATGCCTATGCTTATGGGTCCACCTTCCAACTCCTACAATTTCTTTGGCTTTTGG AGCTGAAGAAGCTCTCTGCCTTGAGCCTACCTCCCAGTTCCTGAAGATTTCCTACTGTCTAGATGCTGCC ACACCTTCCTGCCAGCCAGCCCAGAGGGATGTTCCATCACCTTCACAGTATTTATTATCCTCTGCCACCT CACTGCTCCCATACAGACATCCTTACACAACCAGCAACGCAACAAGGAACACTGAAAAAATGCCTCATTC TAATAAAGTGACTTTCTCCATTACCCTTACCCCTCTACAGACAGATTTAAAAGCAGACAGAACAAATAAA CGAACAGCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584592 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584592 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7299 same embryo
	EMAGE:7301 same embryo
	EMAGE:7300 same embryo
	EMAGE:7298 same embryo
	EMAGE:7303 same embryo
	EurExpress:euxassay_004404 same experiment
	MGI:4824288 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source