EMAGE mouse gene expression spatial database

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EMAGE:7398

Gpr137 G protein-coupled receptor 137 ( MGI:2147529)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004640. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004640_01	euxassay_004640_02	euxassay_004640_03	euxassay_004640_04

euxassay_004640_05	euxassay_004640_06	euxassay_004640_07	euxassay_004640_08	euxassay_004640_09

euxassay_004640_10	euxassay_004640_11	euxassay_004640_12	euxassay_004640_13	euxassay_004640_14

euxassay_004640_15	euxassay_004640_16	euxassay_004640_17	euxassay_004640_18	euxassay_004640_19

euxassay_004640_20	euxassay_004640_21	euxassay_004640_22	euxassay_004640_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7398_wholemount_strong.wlz

7398_wholemount_moderate.wlz

7398_wholemount_weak.wlz

7398_wholemount_possible.wlz

7398_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7398_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 15 16

facial vii ganglion

moderate

regional

moderate expression: see section 20 21 weak expression: see section 03

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 17 18 19 20 21 22 weak expression: see section 03 04

trigeminal v nerve

moderate

regional

moderate expression: see section 09 17

ventral grey horn

weak

regional

weak expression: see section 07 08 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 17 weak expression: see section 06 07 08 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7718
Entity Detected:	Gpr137, G protein-coupled receptor 137 ( MGI:2147529)
Sequence:	sense strand is shown >T7718 GGGAGCTGGAGCCCGGTGTCCCGGCGACCGGATCCCGCTTACCCCGCAGCGGCCTGAGACCTGACATCGA CTTCCTCTTATTCACCGGGGCTGAGGGTGGACGCGGTGGGGGAACACGTCTGGAATCTTGAGGACAGAGG AAGAAGCCTCCCAATCCGTATTTATTTCCCTGGCTTCTGGCCAGCCCTCTATCTCTGTCTCCGGGTTTCA GGCCTCCCTCCTTGACATGGAGAGTAACCTGTCTGGTCTGGTGCCTGCAGCTGGGCTGGTACCTGCACTG CCGCCTACAGTGACCCTGGGGCTGACCGCTGCCTACACTGCCCTATATGCTCTGCTCTTCTTCTCTGTCT ATGCCCAGCTCTGGCTGGTGCTTCTGTATGGGCACAAGCGCCTCAGCTATCAGACAGTATTCCTGGCGCT CTGTCTGCTCTGGGCAGCCTTGCGTACCACACTTTTCTCCTTCTACTTCCGAGATACTCCCAGGGCCAAC CGCCTGGGGCCCTTGCCTTTTTGGCTTCTCTATTGCTGCCCTGTCTGCCTGCAGTTCTTCACACTGACGC TTATGAACCTCTACTTTGTGCAGGTGGTGTTCAAGGCCAAGGCGAAGCGTCGGCCAGAGATGAGCCGAGG CTTGCTGGCTGTCCGAGGGGCCTTCGTGGGTGCTTCACTGCTCTTTTTGCTGGTGAATGTACTGTGTGCA GTGCTGTCTCGCCAGCGCCAGGCACAGCCCTGGGTCCTTCTGCTGGTACGTGTCCTGGTGAGCGACTCTC TCTTCGTCATCTGTGCCCTCTCGCTTGCTGCCTGCCTCTGCCTTGTGGCCCGTCGAGCCCCTTCCACCAG CATCTACTTAGAGGCCAAGGGGACCAGCGTGTGTCAGGCAGCTGCTATAGGGGGTGCCATGGTCCTTCTC TATGCCAGCCGGGCCTGTTACAACCTGGCAGCTCTGGCCTTGGCCCCTCGGAGCCGGCTAGATGCCTTCG ATTACGACTGGTACAACGTATCTGACCAGGCAGATCTGGTGAATGACCTAGGGAACAAAGGCTACCTGGT GTTTGGTCTCATCCTCTTCGTTTGGGAACTGCTGCCCACCACATTGCTGGTGGGCTTCTTCCGGGTACAC CGGCCCCCACAGGATCTGAGCACCAGTCGAATCCTCAATGGGCAGGTTTTTGGCTCCCGATCCTACTTCT TTGACCGGGCTGGCCACTGTGAGGATGAGGGCTGCTCCTGGGAGCATAGCCGGAGTGAGAGCACCAGCAT GTCCGGCAGCCTGGGCTCTGGCAGTTGGTATGGTGCCATCGGACGTGAGCCAGGCTGGGGAGGGGCCAGC CAGACGAGGACCACTCCTCTGCTCTTCTCCCAGGTGCCAGGACCTGGTAGTCACCACCACAGCCTCTATT CTACACCACAGACGTGATCTGCTTTTACTCCCCTTAGAATACCCAGGCCCCAGCTCCCCTGATCCCTGGC CCCTGTGCCAAGTACGTCTGCTGCTTCTTGCCCAGGTTGCCAGGAGACTGTGGCTGCTTCTCTCCTCTGG CCGGCTCCTTGCCGCTCCTATTGTAGTGAGCTTGTGCCATCCCCCTAAAATGGAAACAAGGCCCCAGGAT ACCAGATGCCCACAGTGCCCTGGCATGACCTGCCACCTCTGCTTCCACACTGGAGCCGGCTACCTCTCCT GTGCCTACCACTCAATAAACAGAGGCTGTGCCCTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6308873 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6308873 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7394 same embryo
	EMAGE:7397 same embryo
	EMAGE:7396 same embryo
	EMAGE:7399 same embryo
	EMAGE:7395 same embryo
	EurExpress:euxassay_004640 same experiment
	MGI:4825174 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source