EMAGE mouse gene expression spatial database

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EMAGE:7581

Mmp9 matrix metallopeptidase 9 ( MGI:97011)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004648. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004648_01	euxassay_004648_02	euxassay_004648_03	euxassay_004648_04

euxassay_004648_05	euxassay_004648_06	euxassay_004648_07	euxassay_004648_08	euxassay_004648_09

euxassay_004648_10	euxassay_004648_11	euxassay_004648_12	euxassay_004648_13	euxassay_004648_14

euxassay_004648_15	euxassay_004648_16	euxassay_004648_17	euxassay_004648_18	euxassay_004648_19

euxassay_004648_20	euxassay_004648_21	euxassay_004648_22	euxassay_004648_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7581_wholemount_strong.wlz

7581_wholemount_moderate.wlz

7581_wholemount_weak.wlz

7581_wholemount_possible.wlz

7581_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7581_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 moderate expression: see section 01 02

elbow mesenchyme

strong

regional

strong expression: see section 01

upper arm mesenchyme

strong

regional

strong expression: see section 01 03 04 22 23 moderate expression: see section 02

hand mesenchyme

strong

regional

strong expression: see section 03 23

lower leg mesenchyme

strong

regional

strong expression: see section 01 21 22 23

upper leg mesenchyme

strong

regional

strong expression: see section 01 03 04 18 19 20 21 moderate expression: see section 02

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

not examined

regional

not examined expression: see section 22 23

nasal septum

strong

regional

strong expression: see section 10 11 12 13 14 15 16

viscerocranium

strong

regional

Expression in the turbinate bone.

mandible

strong

single cell

strong expression: see section 04 05 06 07 08 09 10 17 18 19 20 21 22 23

lower jaw incisor

strong

single cell

strong expression: see section 11 12 13 15 16

maxilla

strong

single cell

strong expression: see section 04 05 06 07 08 09 10 17 18 19 20 21 22 23

upper jaw incisor

strong

single cell

strong expression: see section 11 12 13 14 15 16

bladder

moderate

regional

moderate expression: see section 13 weak expression: see section 10 11 12 14

not examined

regional

not examined expression: see section 14

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 moderate expression: see section 23

clavicle

strong

single cell

strong expression: see section 06 07 08 09 17 18 19

scapula

strong

single cell

strong expression: see section 03 04 05 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7721
Entity Detected:	Mmp9, matrix metallopeptidase 9 ( MGI:97011)
Sequence:	sense strand is shown >T7721 CTCACCATGAGTCCCTGGCAGCCCCTGCTCCTGGCTCTCCTGGCTTTCGGCTGCAGCTCTGCTGCCCCTT ACCAGCGCCAGCCGACTTTTGTGGTCTTCCCCAAAGACCTGAAAACCTCCAACCTCACGGACACCCAGCT GGCAGAGGCATACTTGTACCGCTATGGTTACACCCGGGCCGCCCAGATGATGGGAGAGAAGCAGTCTCTA CGGCCGGCTTTGCTGATGCTTCAGAAGCAGCTCTCCCTGCCCCAGACTGGTGAGCTGGACAGCCAGACAC TAAAGGCCATTCGAACACCACGCTGTGGTGTCCCAGACGTGGGTCGATTCCAAACCTTCAAAGGCCTCAA GTGGGACCATCATAACATCACATACTGGATCCAAAACTACTCTGAAGACTTGCCGCGAGACATGATCGAT GACGCCTTCGCGCGCGCCTTCGCGGTGTGGGGCGAGGTGGCACCCCTCACCTTCACCCGCGTGTACGGAC CCGAAGCGGACATTGTCATCCAGTTTGGTGTCGCGGAGCACGGAGACGGGTATCCCTTCGACGGCAAGGA CGGCCTTCTGGCACACGCCTTTCCCCCTGGCGCCGGCGTTCAGGGAGATGCCCATTTCGACGACGACGAG TTGTGGTCGCTGGGCAAAGGCGTCGTGATCCCCACTTACTATGGAAACTCAAATGGTGCCCCATGTCACT TTCCCTTCACCTTCGAGGGACGCTCCTATTCGGCCTGCACCACAGACGGCCGCAACGACGGCACGCCTTG GTGTAGCACAACAGCTGACTACGATAAGGACGGCAAATTTGGTTTCTGCCCTAGTGAGAGACTCTACACG GAGCACGGCAACGGAGAAGGCAAACCCTGTGTGTTCCCGTTCATCTTTGAGGGCCGCTCCTACTCTGCCT GCACCACTAAAGGCCGCTCGGATGGTTACCGCTGGTGCGCCACCACAGCCAACTATGACCAGGATAAACT GTATGGCTTCTGCCCTACCCGAGTGGACGCGACCGTAGTTGGGGGCAACTCGGCAGGAGAGCTGTGCGTC TTCCCCTTCGTCTTCCTGGGCAAGCAGTACTCTTCCTGTACCAGCGACGGCCGCAGGGATGGGCGCCTCT GGTGTGCGACCACATCGAACTTCGACACTGACAAGAAGTGGGGTTTCTGTCCAGACCAAGGGTACAGCCT GTTCCTGGTGGCAGCGCACGAGTTCGGCCATGCACTGGGCTTAGATCATTCCAGCGTGCCGGAAGCGCTC ATGTACCCGCTGTATAGCTACCTCGAGGGCTTCCCTCTGAATAAAGACGACATAGACGGCATCCAGTATC TGTATGGTCGTGGCTCTAAGCCTGACCCAAGGCCTCCAGCCACCACCACAACTGAACCACAGCCGACAGC ACCTCCCACTATGTGTCCCACTATACCTCCCACGGCCTATCCCACAGTGGGCCCCACGGTTGGCCCTACA GGCGCCCCCTCACCTGGCCCCACAAGCAGCCCGTCACCTGGCCCTACAGGCGCCCCCTCACCTGGCCCTA CAGCGCCCCCTACTGCGGGCTCTTCTGAGGCCTCTACAGAGTCTTTGAGTCCGGCAGACAATCCTTGCAA TGTGGATGTTTTTGATGCTATTGCTGAGATCCAGGGCGCTCTGCATTTCTTCAAGGACGGTTGGTACTGG AAGTTCCTGAATCATAGAGGAAGCCCATTACAGGGCCCCTTCCTTACTGCCCGCACGTGGCCAGCCCTGC CTGCAACGCTGGACTCCGCCTTTGAGGATCCGCAGACCAAGAGGGTTTTCTTCTTCTCTGGACGTCAAAT GTGGGTGTACACAGGCAAGACCGTGCTGGGCCCCAGGAGTCTGGATAAGTTGGGTCTAGGCCCAGAGGTA ACCCACGTCAGCGGGCTTCTCCCGCGTCGTCTCGGGAAGGCTCTGCTGTTCAGCAAGGGGCGTGTCTGGA GATTCGACTTGAAGTCTCAGAAGGTGGATCCCCAGAGCGTCATTCGCGTGGATAAGGAGTTCTCTGGTGT GCCCTGGAACTCACACGACATCTTCCAGTACCAAGACAAAGCCTATTTCTGCCATGGCAAATTCTTCTGG CGTGTGAGTTTCCAAAATGAGGTGAACAAGGTGGACCATGAGGTGAACCAGGTGGACGACGTGGGCTACG TGACCTACGACCTCCTGCAGTGCCCTTGAACTAGGGCTCCTTCTTTGCTTCAACCGTGCAGTGCAAGTCT CTAGAGACCACCACCACCACCACCACACACAAACCCCATCCGAGGGAAAGGTGCTAGCTGGCCAGGTACA GACTGGTGATCTCTTCTAGAGACTGGGAAGGAGTGGAGGCAGGCAGGGCTCTCTCTGCCCACCGTCCTTT CTTGTTGGACTGTTTCTAATAAACACGGATCCCCAACCTTTTCCAGCTACTTTAGTCAATCAGCTTATCT GTAGTTGCAGATGCATCCGAGCAAGAAGACAACTTTGTAGGGTGGATTCTGACCTTTTATTTTTGTGTGG CGTCTGAGAATTGAATCAGCTGGCTTTTGTGACAGGCACTTCACCGGCTAAACCACCTCTCCCGACTCCA GCCCTTTTATTTATTATGTATGAGGTTATGTTCACATGCATGTATTTAACCCACAGAATGCTTACTGTGT GTCGGGCGCGGCTCCAACCGCTGCATAAATATTAAGGTATTCAGTTGCCCCTACTGGAAGGTATTATGTA ACTATTTCTCTCTTACATTGGAGAACACCACCGAGCTATCCACTCATCAAACATTTATTGAGAGCATCCC TAGGGAGCCAGGCTCTCTACTGGGCGTTAGGGACAGAAATGTTGGTTCTTCCTTCAAGGATTGCTCAGAG ATTCTCCGTGTCCTGTAAATCTGCTGAAACCAGACCCCAGACTCCTCTCTCTCCCGAGAGTCCAACTCAC TCACTGTGGTTGCTGGCAGCTGCAGCATGCGTATACAGCATGTGTGCTAGAGAGGTAGAGGGGGTCTGTG CGTTATGGTTCAGGTCAGACTGTGTCCTCCAGGTGAGATGACCCCTCAGCTGGAACTGATCCAGGAAGGA TAACCAAGTGTCTTCCTGGCAGTCTTTTTTAAATAAATGAATAAATGAATATTTACTTAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6309245 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6309245 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7583 same embryo
	EMAGE:7582 same embryo
	EMAGE:7580 same embryo
	EMAGE:7585 same embryo
	EMAGE:7579 same embryo
	EMAGE:7584 same embryo
	EurExpress:euxassay_004648 same experiment
	MGI:4826414 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source