EMAGE mouse gene expression spatial database

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EMAGE:7631

Lhfp lipoma HMGIC fusion partner ( MGI:1920048)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004723. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004723_01	euxassay_004723_02	euxassay_004723_03	euxassay_004723_04

euxassay_004723_05	euxassay_004723_06	euxassay_004723_07	euxassay_004723_08	euxassay_004723_09

euxassay_004723_10	euxassay_004723_11	euxassay_004723_12	euxassay_004723_13	euxassay_004723_14

euxassay_004723_15	euxassay_004723_16	euxassay_004723_17	euxassay_004723_18	euxassay_004723_19

euxassay_004723_20	euxassay_004723_21	euxassay_004723_22	euxassay_004723_23	euxassay_004723_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7631_wholemount_strong.wlz

7631_wholemount_moderate.wlz

7631_wholemount_weak.wlz

7631_wholemount_possible.wlz

7631_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7631_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

regional

moderate expression: see section 18 19 20 21 22 23 24

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

epidermis

strong

regional

strong expression: see section 07

vibrissa

strong

regional

strong expression: see section 05 06 08 21 22 23

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 17 18

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15

diencephalon meninges

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

thalamus

moderate

regional

moderate expression: see section 08 09 10 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23 24

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

olfactory lobe

moderate

regional

moderate expression: see section 11 12 13 16 17 18

telencephalon ventricular layer

moderate

regional

moderate expression: see section 06 07 14 15 21 22 23

medulla oblongata lateral wall

moderate

regional

moderate expression: see section 10 11 12 13 14 15

hindbrain meninges

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

metencephalon lateral wall

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

moderate

regional

moderate expression: see section 08 09 17 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

spinal cord lateral wall

moderate

regional

moderate expression: see section 11

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12

metanephros

moderate

spotted

moderate expression: see section 06 07 08 09 15 16 17 18 19

tail mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8712
Entity Detected:	Lhfp, lipoma HMGIC fusion partner ( MGI:1920048)
Sequence:	sense strand is shown >T8712 CCGGCATCCACCGTGTTCCCGGGCCGCACGGCCGCAGCCGGCGGGCTCTCCGGGTGGGTCCCCCTGACCC TCCACCCCAGCTCTCCAGACTCGCCTCGATCTCCGTCGGGATAGTGGCAAAGGCAAGAAAGACAGTCTTC TTTGGTGCCGCGCTGTCCCCGCCAGGGAAAGACAGCAAGTTGTGCTGCGCCCGGGACTCGCGAGGGACCG CGGCCGCCGCCCGACCGGGGGCCACCAGCCATCTTCACTCCAAAGTATGAGAAGCTGATCGCGGTAATAC TGAAACTCTGTTACGGCAAGTAGCTACATACGCCTTGAAGAGCGGTGGACTACCTGGGAGTTTCTTGTGA CTACACGCCTTCCTTCACACCTTTAGTGCAATCACAGCGGACCCTCATTGCACTGCCTGTTGGAAGATGG CGTCCAGCCTGACTTGTACTGGGGTGATCTGGGCTTTGCTTTCCTTCCTTTCCGCTGCCACCTCCTGTGT GGGGTTCTTCATGCCATACTGGCTCTGGGGGTCTCAGCTAGGCAAGCCTGTATCCTTTGGCACTTTCCGG AGGTGCTCCTATCCTGTTCACGATGAGAGTCGGCAGATGATGGTGATGGTGGAGGAGTGTGGGCGCTATG CCTCCTTCCAGGGCATCCCCAGCACCGAGTGGAGGATCTGTACCATAGTGACAGGCCTTGGCTGCGGCCT GTTGCTCCTGGTGGCTCTCACTGCCCTGATGGGTTGCTGCGTGTCGGAACTCATCTCCAGGACTGTAGGA AGAGTGGCTGGGGGAATCCAGTTCCTGGGGGGCTTGCTGATTGGTGCTGGCTGTGCCCTCTACCCCTTGG GCTGGGACAGTGAGGAAGTCCGGCAGACTTGTGGCTACATCTCTGGCCAGTTTGACTTGGGCAAGTGTGA GATCGGCTGGGCCTACTACTGCACCGGGGCAGGGGCTGCGGCTGCCATGCTGCTGTGTACATGGATGGCT TGCTTCTCAGGCAAGAAGCAAAAGCACTACCCCTACTGAGGTGGTCAAGAACGAGCTGAGGAGAGGACAG ACCACCGGAGACCAGAGGGCCCGAAGCATCCAGCGACTTTCACAAGGTGGAATAGTGGTTGTAAACCCAG AACAGTGTTAATGTGATGAAATTCGGTTGAATCTGGAACATTCTATAGGAAGGGAGGGTGGTGGGGACAT TGTCATTGTCAAAAGCATGGAGAATAAGGGACGGTGGAGGAAAATGGACCAAAGGCTAAAAACATTGCAG GGCATTGTTGTTTCTATTCCACAGAGTACCGTTAATGTATATCATATACCCAGCAAATCTATATATGCAA ACGAGGATCTCATTTTTGTTCTCCTTCAAAAAGATGAGGACTAGGGGAGTCAAAAGGGCTAGTAGAAACA GTATTATCTTAGGAAGCAGGATGAGTACCCTGCAGACATTCCCTGTAGATGAGGATTGAGCCTGAAAGCA CATGAGTACGTGCACACATATGCACACATCATATTCACATACCACATGCACATGTTATATGCACAGGTCA TACGCACATGCCATATGCATACATCACACGCACACATCATACGCATATGTCATCACACCACTGCACAGGG GGTCAGCTGTGTACTGTTGCTCCTGTTTCTTTTCTTTTTTTTAATATACATCAGGACAGCATCATCACTA AGCATACATTAAGCCTAGTCCATGGACCAATATGCCACTCTATCAGTATTTTTGATATCCTGCATAAACT CTTTATGCCCGCAGCATATCTTTTAGTGTTTATGCAAATCTTTAGAGGAAAGCCTTCAAATGTCAATGTT ATTCAAAGACACACAGTAGTGAACTGAGTAGCTTCTGCTGTAACAACCTCACGTAGAACAGGACAGCTTT TGTCTACTATAGGTGAGAATTCAGTTGCAGATTTGAGAGTGAGGAGAGCTTTCTCCAGTTGTTGGATCTT CTTTTCTTTTCTCCATTATTCTACTGTGCTATACCATGATTATTTCAATATTCATTTTGTAAAGGGAAAA AAAAGTACTATTTTGTTTGTATTTGAAAAGCTCTGTGAATAAATTCTCTTTGATCAATAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402782 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402782 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7634 same embryo
	EMAGE:7632 same embryo
	EMAGE:7633 same embryo
	EMAGE:7636 same embryo
	EMAGE:7635 same embryo
	EurExpress:euxassay_004723 same experiment
	MGI:4825917 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source