EMAGE mouse gene expression spatial database

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EMAGE:7669

Gpr85 G protein-coupled receptor 85 ( MGI:1927851)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005306. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005306_01	euxassay_005306_02	euxassay_005306_03	euxassay_005306_04

euxassay_005306_05	euxassay_005306_06	euxassay_005306_07	euxassay_005306_08	euxassay_005306_09

euxassay_005306_10	euxassay_005306_11	euxassay_005306_12	euxassay_005306_13	euxassay_005306_14

euxassay_005306_15	euxassay_005306_16	euxassay_005306_17	euxassay_005306_18	euxassay_005306_19

euxassay_005306_20	euxassay_005306_21	euxassay_005306_22	euxassay_005306_23	euxassay_005306_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7669_wholemount_strong.wlz

7669_wholemount_moderate.wlz

7669_wholemount_weak.wlz

7669_wholemount_possible.wlz

7669_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7669_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 19

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20

vagus x ganglion

weak

regional

weak expression: see section 08 17 18

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 08 17 18 19

trigeminal v nerve

weak

regional

weak expression: see section 08 16

spinal cord

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16

not examined

regional

not examined expression: see section 01 02 03 22 23 24

neural retina

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 22 23 24

tongue muscle

weak

regional

weak expression: see section 10 11 12 13 14 15 16

trachea

weak

regional

weak expression: see section 13 14

axial skeleton

moderate

regional

moderate expression: see section 12 13 14

axial skeleton tail region

moderate

regional

moderate expression: see section 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8940
Entity Detected:	Gpr85, G protein-coupled receptor 85 ( MGI:1927851)
Sequence:	sense strand is shown >T8940 AGCTTGCAGGAAGACAAGCAGACGTTTCACCTTGGGAATTAATGAAAGCGTGCCTGCTAGCTTTTAAGGG GTGAAGAGTGGGTAGTTGTTTAAAATCTTACCCCACCCTCGCAAAAACTCTTTGGAAATGGGAGAATCGA GACGTGGATGATTCTTGGGGCGTTCCTAATCACCAGGTGTCAGACTATGTGGTGTTTGTGGACTGGTCTA GCGTGCCAAATCAAAGATCAGCAGGGGGAAAAATCATTTGAGAAGAATTCTACAAGGCGCATCCAATGCT TAGCAGGACCACCCTGACAGAGAAAGGAAGACGATGTTTGGGGAAGGCGAGGGTTCGGGGCAGCTGGGGC GGAGGCGGCCGTCCGCGGCACGGTGGCTTCGTGCGCTGCCCTAGGGGAAGAGGATGGGCCTGAGGTCCGG GAAGAGCCTTCCCGAGCCCCCCACTGCCAATGCATGCGGGGAGGGAAAAGTTCCGAAGCTGCCTCGAGGG AACAAAAGCCTGATTTGGGGCCGGCTGAACTGGACAGGACTTTGAAGCAAAGCCAGCCTGCTCCGGAGAC AGGCAGGGGTTCCTGCTGGCTCGGCGGAGGCCGGAGCAAGCCTCGCCGCCGCCCTCGCCACCGCGCCGCC GCCGCCGCGGCCCCTGCGTTACTGGGGGAGACGGTGCCGCTGCGACAGGGGAGGACGCGGCTGGAGCTGA AGGAGGCTCCAACGCGCAGGCGCCGCCGAGCCCTGCCTGGATGCTCAGTCAAGGCACTCAGGAAAAAAAA ATCAGCAATTTATAGAAAGAAGAAGCTATAGTCTGTCGGGATATCCAACACCAACAGGGGTGGTGGATTT CAGGTTTCGTAATGTTGCATGATGCGATCGGTATTTACCCGGGGATGTACCGGTTGGGAAATTAACACCA TCGGATGCAGTTCTGCCTGGGCTTCCATCAGCATATTCCAGATGTTCTGATGGCAAATCAAACGGAAGGC AAGAGGAAGCATGGCCACAGATCGGATCTGATCTCTTTGTGGATTACATTTTCAGTAAAATGTATGGATC TATCTTTTCCTTGTTCTTATATCTAGATCATGAGACTTGACTGAGGCTGTTTCCTTATCCTCCATCCATC TATGGCGAACTATAGCCATGCAGCCGACAACATTTTGCAAAATCTCTCGCCTCTAACAGCCTTTCTGAAA CTGACTTCCCTGGGTTTCATAATAGGAGTCAGCGTTGTGGGCAACCTTCTGATCTCCATTTTGCTAGTGA AAGATAAGACCTTGCATAGAGCTCCTTACTACTTCCTGCTGGATCTGTGCTGCTCAGACATCCTCAGATC TGCAATTTGTTTTCCATTTGTATTCAACTCTGTCAAAAATGGCTCCACCTGGACTTACGGGACTCTGACT TGCAAAGTGATTGCCTTCCTGGGGGTTTTGTCCTGTTTCCACACTGCCTTCATGCTCTTCTGCATCAGCG TCACCAGATACTTAGCCATCGCCCATCACCGCTTCTATACAAAGAGGCTGACCTTTTGGACGTGTTTGGC TGTGATCTGCATGGTGTGGACTCTGTCCGTGGCCATGGCATTCCCCCCAGTTTTAGATGTAGGCACCTAC TCCTTCATTAGGGAGGAGGATCAATGCACCTTCCAACACCGCTCCTTCAGGGCTAACGATTCCCTAGGAT TTATGCTGCTCCTTGCTCTCATCCTCCTAGCCACACAGCTTGTCTACCTCAAGCTGATATTTTTTGTCCA CGATCGAAGGAAAATGAAGCCAGTCCAGTTTGTAGCAGCAGTGAGTCAGAACTGGACCTTTCATGGCCCT GGAGCTAGCGGCCAGGCAGCTGCCAATTGGCTAGCAGGATTTGGAAGGGGTCCCACACCACCCACCTTGC TGGGCATCAGGCAAAATGCGAATACCACAGGCAGAAGGCGGCTATTGGTCTTGGATGAGTTCAAAATGGA GAAAAGAATCAGCAGAATGTTTTATATAATGACTTTCCTCTTCCTAACCTTGTGGGGTCCCTACCTGGTG GCCTGCTATTGGAGAGTTTTTGCACGAGGGCCTGTAGTACCAGGGGGATTTCTAACAGCCGCTGTCTGGA TGAGTTTCGCCCAAGCAGGAATCAATCCCTTTGTCTGCATTTTCTCCAACAGGGAGCTGAGGCGCTGTTT CAGCACAACCCTTCTTTACTGCAGAAAATCCAGGTTACCAAGGGAACCTTACTGTGTTATATGAGGGAGC ATCTGTAAATCTTTAGCCTTGTGAAACACTAACCCTCTCTGCTAAGCAATTGTGGCCTGTAGCCATGTCT GGAGAAGAAAACCAAGGATGGGAATCAGCAGTGACAAGGATTTGGGCAACATTCTGCAGTCTTTGCAATA GTTCACCTATAATCCTATTTTAAATCTCGGAGTGATCCTGCTGACTGCTGGCGAAGGTTTGTAATTAAGA AAGGACAGAACCACTGCCCTAAGTTTCTTTCTGTGGGTGAACACTAGATAACGAAAGTAGCAGGTGCTAA GTATCAGTGCTAAATGCTGTGTATATCATTACATATGAAAAAAAACTTCAAAAAACAATTAGCATTGGAC ATTTTAATAAATTAAGTTGACATGAGGTAAATGTGTTGATAAAAGCTAATTTTAGAAGTTTGAAGACTTT AAAACATTTCATACTATTTTTGTTTTGCAAAGACTACACTACTGGGGGACTTAACAGTACTGTAATTCTT AAAAGATGTGCCATGAATTATTGGGGTACACCACTTTAAACACGCCTTGTAAGTTTGGGGGAGCATTCCA AAGCAGTATATTGGTTCCAATTAGAGTTTACCTTTTTTGTATTAATATATTGCTATTTCTAAATACCACT TTCCTTCCCTACCAGTGAAATTGCTAGCATTGAACTGTATTATGTGTTTTTTGTTGATTCAGTAAAAAAA AAGAAAAAGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6418997 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418997 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7666 same embryo
	EMAGE:7670 same embryo
	EMAGE:7668 same embryo
	EMAGE:7667 same embryo
	EMAGE:7671 same embryo
	EurExpress:euxassay_005306 same experiment
	MGI:4825195 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source