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EMAGE:7713

Gpc1 glypican 1 ( MGI:1194891)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713
"Pseudo-wholemount" of euxassay_007466. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007466_01 euxassay_007466_02 euxassay_007466_03 euxassay_007466_04
EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713
euxassay_007466_05 euxassay_007466_06 euxassay_007466_07 euxassay_007466_08 euxassay_007466_09
EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713
euxassay_007466_10 euxassay_007466_11 euxassay_007466_12 euxassay_007466_13 euxassay_007466_14
EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713
euxassay_007466_15 euxassay_007466_16 euxassay_007466_17 euxassay_007466_18 euxassay_007466_19
EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713 EMAGE:7713
euxassay_007466_20 euxassay_007466_21 euxassay_007466_22 euxassay_007466_23 euxassay_007466_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7713Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7713_wholemount_strong.wlz
7713_wholemount_moderate.wlz
7713_wholemount_weak.wlz
7713_wholemount_possible.wlz
7713_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7713_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 13 17 18 19 20 21 22 23 24
diaphragm
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20 21 22 23 24 moderate expression: see section 10 11 15 weak expression: see section 16
radius
moderate moderate
regionalmoderate expression: see section 01
ulna
moderate moderate
regionalmoderate expression: see section 01
forearm rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 02
shoulder rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
humerus
moderate moderate
regionalmoderate expression: see section 02 03 04 05 23 24
hindlimb digit 2 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 08
hindlimb digit 3 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 08
hindlimb digit 4 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 08
hindlimb digit 5 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 08
fibula
moderate moderate
regionalmoderate expression: see section 01 02 24
tibia
moderate moderate
regionalmoderate expression: see section 01 02 24
lower leg rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 03 23
femur
weak weak
regionalweak expression: see section 03 04 19 20 21 22 23
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23 24 moderate expression: see section 14 15 16
thymus primordium
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 12 14 15 16
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 08 09 10 18 19 20 21
pancreas
moderate moderate
regionalmoderate expression: see section 08 09 10 weak expression: see section 07
vibrissa
strong strong
regionalstrong expression: see section 09 21 moderate expression: see section 05 06 07 08 22 weak expression: see section 20
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 weak expression: see section 13 14
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 weak expression: see section 13 14
olfactory cortex ventricular layer
weak weak
homogeneousweak expression: see section 09 10 14 15 16
telencephalon ventricular layer
weak weak
homogeneousweak expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22 23
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 16 17
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 03 04 05 moderate expression: see section 06 07 08 09 10 11 12 weak expression: see section 13 14 15 16
pons mantle layer
moderate moderate
regionalmoderate expression: see section 08 weak expression: see section 07 18
midbrain marginal layer
moderate moderate
regionalmoderate expression: see section 12
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 weak expression: see section 13 14 15 16
facial vii ganglion
strong strong
regionalstrong expression: see section 04 05 06 20 21 22
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 19
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 moderate expression: see section 17
vagus x ganglion
strong strong
regionalstrong expression: see section 09 18
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 19 20
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 16 17
ventral grey horn
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 10 17 18
cervical ganglion
moderate moderate
regionalmoderate expression: see section 10 11 17
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 12
dorsal root ganglion
strong strong
regionalstrong expression: see section 07 08 09 13 17 moderate expression: see section 10 14 15 16
saccule
strong strong
regionalstrong expression: see section 05 06 07 18 19 20 21 moderate expression: see section 08
cochlea
moderate moderate
regionalmoderate expression: see section 09 10 17
extrinsic ocular muscle
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 19 20 21 22 23 24
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 24 weak expression: see section 04 23
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 08 09 10 11 15 16 18
heart valve
moderate moderate
regionalmoderate expression: see section 12 13 14 15
tongue muscle
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17
stomach
moderate moderate
regionalmoderate expression: see section 05 06 weak expression: see section 01 02 03 04 07 08 09
midgut
weak weak
regionalweak expression: see section 07 08 09 10 11 14 17 18
lower lip
moderate moderate
regionalmoderate expression: see section 07 08 09 10 19 21 22 weak expression: see section 20
mandible
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23 moderate expression: see section 04
lower jaw incisor
strong strong
regionalstrong expression: see section 12 13 16
lower jaw molar
strong strong
regionalstrong expression: see section 08 09 19
upper lip
moderate moderate
regionalmoderate expression: see section 06 07 08 09 21 22 weak expression: see section 20
maxilla
strong strong
regionalstrong expression: see section 06 07 08 09 10 17 18 19 20 21
upper jaw incisor
strong strong
regionalstrong expression: see section 12 13 16
upper jaw molar
strong strong
regionalstrong expression: see section 08 09 19
metanephros
strong strong
regionalstrong expression: see section 06 07 16 17 18 moderate expression: see section 08 weak expression: see section 09
lung
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 14 15 17 18 19 20 21 22 23 weak expression: see section 03 04 12 13 16 24
nucleus pulposus
strong strong
regionalstrong expression: see section 11 12 moderate expression: see section 13
exoccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 20 21 22 24
vault of skull
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23 24
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 19 20 21 22 23 24 moderate expression: see section 12 13
clavicle
strong strong
regionalstrong expression: see section 09 10 11 17 18 moderate expression: see section 19
sternum
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 16 17 18 19 20
pelvic girdle skeleton
weak weak
regionalweak expression: see section 08 15
tail mesenchyme
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8973
Entity Detected:Gpc1, glypican 1 ( MGI:1194891)
Sequence:sense strand is shown

>T8973
CGCACACTGGGCTGAGCGGGCCGGCCGGGACCGAGGGAGGCGCGCACCGGCGGCCAGGGAGCCTCGGGCC
CCGCCATGGAACTCCGGACCCGAGGCTGGTGGCTGCTGTGCGCGGCCGCCGCGCTGGTCGTCTGCGCCCG
CGGGGACCCCGCCAGCAAGAGCCGGAGCTGCAGCGAAGTCCGCCAGATCTACGGGGCTAAGGGCTTTAGC
CTGAGCGATGTGCCTCAGGCAGAGATCTCGGGTGAGCACCTGCGGATCTGCCCCCAGGGCTACACTTGCT
GTACTAGTGAGATGGAGGAGAATTTGGCCAACCACAGCCGAATGGAGCTGGAGAGCGCACTCCATGACAG
CAGCCGCGCCCTGCAGGCCACACTGGCCACCCAGCTGCATGGCATCGATGACCACTTCCAGCGCCTGCTG
AATGACTCGGAGCGCACACTGCAGGAGGCTTTCCCTGGGGCCTTTGGGGACCTGTATACGCAGAACACTC
GTGCCTTCCGGGACCTATATGCTGAGCTGCGCCTCTACTACCGTGGGGCCAACCTGCACCTTGAGGAGAC
GCTGGCCGAGTTCTGGGCACGGCTGCTGGAGCGCCTCTTCAAGCAGCTGCACCCCCAGCTGCTGCCTGAT
GACTACCTGGACTGCCTGGGCAAGCAGGCGGAGGCACTGCGGCCGTTTGGAGATGCCCCTCGAGAACTGC
GCCTGCGGGCCACCCGTGCCTTTGTGGCTGCACGTTCCTTTGTGCAGGGCCTGGGTGTGGCCAGTGATGT
AGTCCGGAAGGTGGCCCAGGTACCTCTGGCCCCAGAATGTTCTCGGGCCATCATGAAGTTGGTCTACTGT
GCTCATTGCCGGGGAGTCCCGGGCGCCCGGCCCTGCCCCGACTATTGCCGAAATGTGCTCAAAGGCTGCC
TTGCCAACCAGGCCGACCTGGATGCCGAGTGGAGGAACCTCCTGGACTCCATGGTGCTCATCACTGACAA
GTTCTGGGGCCCGTCGGGTGCGGAGAGTGTCATTGGCGGTGTGCACGTGTGGCTGGCGGAGGCCATCAAC
GCCCTCCAGGACAACAAGGACACACTCACAGCTAAGGTCATCCAGGCCTGTGGAAACCCCAAGGTCAATC
CCCACGGCTCTGGGCCCGAGGAGAAGCGTCGCCGTGGCAAATTGGCACTGCAGGAGAAGCCCTCCACAGG
TACTCTGGAAAAACTGGTCTCTGAGGCCAAGGCCCAGCTCCGAGACATTCAGGACTTCTGGATCAGCCTC
CCAGGGACACTGTGCAGTGAGAAGATGGCCATGAGTCCTGCCAGTGACGACCGCTGCTGGAATGGAATTT
CCAAGGGCCGGTACCTACCAGAGGTGATGGGTGACGGGCTGGCCAACCAGATCAACAACCCTGAGGTGGA
AGTGGACATCACCAAGCCAGACATGACCATCCGCCAGCAGATTATGCAGCTCAAGATCATGACCAACCGT
TTACGTGGCGCCTATGGCGGCAACGACGTGGACTTCCAGGATGCTAGTGATGACGGCAGTGGCTCCGGCA
GCGGTGGCGGATGCCCAGATGACACCTGTGGCCGGAGGGTCAGCAAGAAGAGTTCCAGCTCCCGGACCCC
CTTGACCCATGCCCTCCCCGGCCTGTCAGAACAGGAGGGACAGAAGACCTCAGCTGCCACCTGCCCAGAG
CCCCACAGCTTCTTCCTGCTCTTCCTCGTCACCTTGGTCCTTGCGGCAGCCAGGCCCAGGTGGCGGTAAC
TGCCCCCTATCCCCAAGGACTGTCTTGGCCAAAACATGCAACAGACCATATTTAATTCCCTTGGCCTTTT
GAGGTCCAGGGCAGGATAAGGAGACAGTAGCTCTGAGTGCTGGGGCAGGGTGCATGGGGGGGTCCTGGCC
TCCTGGGTCTGGCCACGCCTGTCACCCTAGCTTTTAATTTTGTATCAGGTCAGCTGCGAGCCAGTGTCCC
CAAAAGCCATGTATTTCAGGGACCTCAGGGGCACCTCTGGCTGCACACTCCTCCCCTACCCTCCTGCACC
ACCCCCAGAAGCTCATGAGGCCAACCAGAAGGGCGGCTGTTAGCTACAACCCAACGGAGACCTCAAGTGA
GCCTGTGCCTTCCTCTCCTGCCTCTTCCCGCTGGGGACTCCCTACCAGACCCCATGGGCCACAGATGTCA
GAAATTGAGGCCCATCCTGCAGCTCCCCAGGAAGCCTGCAAGGGATGCCAGTATGTTGCTGTCCAGGCTC
TGGCAGGGCCTTCAAGTTTATGCATGATACCTTCTACTCAGAAGAAGCTCTGCAGCCAGGATCCCCACAC
ACAGCAGACCAGGACCCTGTACCACTGTGGAGCTCAGTGAATCTGGTTCTCAAAAAAAGACCCACTGTGG
GATTCTCTAGTATGACGTAGGTGAGGTGGGAGGTGGCAGCTGGCACAGCCATACTACGCTCAACTCTTCC
ACACTGTTTGAGGGTATGTTCCGTCCCCTCAGATGGGCTCACAAAGACTGGCCTTGGTGGTCCACATGAG
GAGCCTCACTTCAGGGGAACAGCCAGCTACATCTAGCCCCTACCCCACTTAGAAAGGTCACCTGGCCATG
GGGATGCTGGGTGGCTGGTGAAACTTTAGCTCAGGGTCAATGAGACCTGGCACTGCAGGTCAAGAGGATG
CCTGGGCCCCTTCCTGACCCAGGCAGCTCTGCAGGAGCTGGGGCAGCATTGACAGTCAAGGGCTTTTACA
GACATGCACATTCAGACCCTCAGTGTCCTTGTCCACTGAGCGCTACATCTCCATCTTCCTTGACAGCACT
GGAGAGGCTCAGGGCACCACTTGGAATCTGGTTTCCTCCTATGGCCTCGAGACCTGGGGGCACCTGGCTT
ACCAAGGCTGTCAAGGCTCCATCTTCCCTTCCAGGGCCTAGGGGATGCTGAGTTGCTATATCCAGGAAGG
CAGTGGTCCTGACATGCAGAAGGCCTGGAAAAAAGGCTGCTTACCGCTTTTTTTTTTTTTTAATTTTAAT
TTTTTTTTTTTAACTTTCTTTTAGTCACTGGGTTAGAGAGGCTATTGCCAGCACAGCACCAAGGCCTGCC
CAGTAGTCAGGTCCCCTGGTCCAGGATGGGTATTCTCCATAATTGGTCTGTGGGTTTGTTGGGCCCATGC
CCTGAGCACACTCAACCCAGCCACAGCCCTGCTAGTGCCACTCAAGCAGGGGCACTGGGAGCTAGACCTG
GGCTCTGAGATGAGTGCCTAGGTGTCAGGACAGCAGCACCCATGCTGTACAGGGCAGCCGGGACAGTGGC
CAGGGTACTGGGTCCTTTCTCTGTCACTCGGTGTCAGTGGATAATGTGTGTTCTTTGAGTCCTTGTATGA
ATTAAAGGCTGGGGACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6810413 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810413 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7714 same embryo
 EMAGE:7712 same embryo
 EMAGE:7715 same embryo
 EMAGE:7716 same embryo
 EMAGE:7711 same embryo
 EurExpress:euxassay_007466 same experiment
 MGI:4825154 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS