EMAGE mouse gene expression spatial database

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EMAGE:7716

C2cd2l C2 calcium-dependent domain containing 2-like ( MGI:1919014)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007479. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007479_01	euxassay_007479_02	euxassay_007479_03	euxassay_007479_04

euxassay_007479_05	euxassay_007479_06	euxassay_007479_07	euxassay_007479_08	euxassay_007479_09

euxassay_007479_10	euxassay_007479_11	euxassay_007479_12	euxassay_007479_13	euxassay_007479_14

euxassay_007479_15	euxassay_007479_16	euxassay_007479_17	euxassay_007479_18	euxassay_007479_19

euxassay_007479_20	euxassay_007479_21	euxassay_007479_22	euxassay_007479_23	euxassay_007479_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7716_wholemount_strong.wlz

7716_wholemount_moderate.wlz

7716_wholemount_weak.wlz

7716_wholemount_possible.wlz

7716_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7716_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 20 21 22 23 24 weak expression: see section 07 18 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 21 22 weak expression: see section 06

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 21 22 weak expression: see section 06 07 08 09 17 18 19 20

vagus x ganglion

weak

regional

weak expression: see section 09 18

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 19

trigeminal v nerve

weak

regional

weak expression: see section 09 17

ventral grey horn

moderate

regional

moderate expression: see section 10 11 13 15 weak expression: see section 12 14

neural retina

moderate

regional

moderate expression: see section 01 02 03 23 24

mandible

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23

maxilla

weak

regional

weak expression: see section 08 09 10 18 19 20

clavicle

moderate

regional

moderate expression: see section 09 10 17 18 weak expression: see section 19 not examined expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8974
Entity Detected:	C2cd2l, C2 calcium-dependent domain containing 2-like ( MGI:1919014)
Sequence:	sense strand is shown >T8974 GGAACCTAGCTGGGGTCACACCGCCGGTGTTAGGGATCCAGATCTCTGGCTACGGAGACAGAGCTCGAGC GTCGCGACCCCGGAGGACCTCTTCTCCATCCGTGGCACCCACTGGTCCTGGAATCTCGGCGGCGCCGGAG AGCCCCGGAGCCCGCGCGCTCAGCCCCGGGGGAGCTCGCCCACGCCCCACGGTCCATCCGGGCCATGCTC CCCCGGGGCAGCGGCTGAACCCGAGCCGGGACCCGAGCCCGCGGGAGCATGGATCCGGACTGGGGGCAGC GGGATGTGGGCTGGGCGGCCCTGCTGGTTCTCTTCGCCGCCTCGCTGATCACGGTATTGGGCTGGATGCT GCAGTATGCCCGGGGTTTGTGGCTGTCGCGAGCCGATGGGGGCCGAGACTCCCGACCTGCCTCAGCTGCT GAGCCCGGGGGTTCACTGCGCGAGCTGGGTGTGTGGCGTTCGCTGCTGCGTTTGCGGGCGACCCGGACCA GCACCCCCGAGGAAGCCGGCGTACGGGGCCTCCTGGCTTCGCTCTTTGCCTTCAAGTCTTTCCGGGAGAA CTGGCAACGGGCTTGGGTGCGAGCCTTGAATGAGCAGGCCTGCAGGGACGGGAGCTCCATCCAAATCGCC TTTGAAGAGATACCCCAACTCCCACCAAGAGCCAGCATCAGTCATGTGACCTGCGTTGACCAATCAGAGC GCACCATGGTGCTGCATTGCCAGCTCTCTGCTGAGGAGGTGCGCTTCCCCATCTCTGTGACCCAGCAGTC CCCCGCTGCCGTCTCCATGGAGACCTACCACGTCACTCTGACACTGCCACCAACACAGTTGGAAGTCAGC CTGGAGGAAATCCCTGATGAGGGGCTCCTGGTGTCCTGGGCCTTCACTGACCGCCCAGAACTCAGCCTAA AGGTGCTTCCCAAGTTGCAGACTAGGGAGAGAGATGAGGAACAACCAGAGCTCTCAACAGTTGAGGAACT GATCAAGGACGCTATAGTCAGCACTCAGCCCGCCATGATGGTCAACCTCAGGGCCTGCTCTGCCCCAGGA GGCCTGGTACCCAGTGAGAAGCCACCCACGATGTCCCAGGCCCAGCCATCCATCCCCAGACCTACCCGAT TATTCTTACGGCAGCTTCGAGCATCTCACCTGGGAAGTGAGCTAGGAGGTACTGAGGAACTGTGCTGTGC CGCTGAGCTTGACAACCCCATGCAACAAAAGTGGACCAAACCCATGAGGGCGGGCCCCGAGGTGGAATGG ACCGAGGACCTAGCTCTGGATCTGGGTCCCCAGAGCCGGGAGCTGACCCTCAAAGTGCTCCGGAGCAGCA GCTGTGGAGATGCTGAACTCCTTGGCCAAGCCACACTGCCTGTGGGCTCACCCTCTAGACCGATGTCACG AAGACAGGTGTGCCCACTGACTCCAGGGCCCGGGAAATCCCTGAGCCCGGCAGCCACCGTGACAGCGGAG CTACATTATGAGCAGGGTTCCCCTCGGAATCTGGGCACGCCCACCTCCTCCACCCCTCGCCCCAGCATCA CACCCACCAAGAAGATTGAGTTGGACCGGACCATCATGCCCGACGGCACAGTCGTCACCACTGTCACTAC CGTCCAGTCCCGCCCCCGTGTAGATGGCAAACTAGACTCCCCCTCCCGCTCCCCGTCCAAGGTGGAGGTG ACTGAGAAGATGACAACCGTGCTGAGTGAGAGCAGCGGCCCCAGCAATGCCTCCCACAGCAGCAGCCGGG AGAGCCACCTTTCCAATGGCTTGGATCCAGTAGCAGAGACAGCCATTCGCCAGCTGACTGAGCCCAGTGG GCGGGCAGCCAAGAAGACACCCACCAAGAGGAGCACGCTCATCATCTCTGGTGTTTCCAAGGTGCCCATC GCCCAGGACGAGTTGGTTCTCTCCTTGGGTTACGCGGCATCTCTGGAAGCCTCGATGCAAGATGATGCAG GAACCAGTGGTGGTCCTTCGTCACCTCCCTCAGACCCCTCAGCCACATCCCCAGGACCTGTTGATGCCCT CTCCAGTCCCACAAGTGTCCAGGAGGCAGATGAGACAACACGTTCAGACATCTCTGAGAGGCCGTCTGTG GATGATGTTGAGTCAGAAACAGGGTCCACTGGTGCCCTGGAGACCAGAAGCCTCAAGGATCACAAAGTGA GTTTCCTGCGCAGTGGCACAAAGCTCATCTTCCGCCGGAGGCCCCGACAGAAGGAAGCTGGTCTGAGCCA GTCACACGATGACCTGTCCAACACGACGGCCACACCTAGCGTCCGGAAGAAGGCTGGCAGCTTTTCCCGT CGCCTTATCAAGCGTTTTTCCTTCAAATCCAAACCCAAGGCCAATGGCAACCCTAGCCCCCAGCTCTGAA AGCCCCCGAGCTAGGAGAGATCAAGAGTTCTCTTAGTCTGTCCCCCACATCCCCTTCTGTACCCCTTCCT GGATCCCCACTGCTGGGGCCAGGAAAGCCCTCGGGTTATAGGGAGCCCCAGCACACTGGGCTGTGGGGTG AGCGGGAAGGGTGTCTGACATGGGAGGCATCTGGGAGGCAACAGTTGTTGCGTGAGCGTAGAGGAGAGTT TGGCAGCTGAGAGTCAGGGGCAAGCTCTGTCTTGGGCGTGTGGACACTCATCAGAAGCCTCTGCTGGTAC TGCCGCCAGAGGGGAGCCCAGAGTACTCTCTCATCAGCAGGTCCTTCTCCCTTATTTATTCTCGTTTCTA TTTATATGTGTGGTCTAGAACCCTTGGCAAACAGATGATAGAGGGCATCTCTCCCAGGTGACCCTTTTCT GTCCCAGGAGGGTAAGGCAATTCCCAGGACGGGTTTCTACCCCTCCCTTGGGAGTCCTCTCTTAGACCAG CACCAATGTCTGCCTTCAGAAGACATTGGCAGATCACAGGAAGCCGGGCTAGGGCCCGGGATGGGGGGCA GGCACTCCCCACCTCCCTACCTCCCACATCGCTCCTTGTCTCTCCTTCCCCTTTATTACCATTTTGTACT TGATGCCTTCTCCATGAGCAGTGGCTCAGTTGGAAGGAGGGAGCCAGGAAGCTGGGAGGAAGCCTTCCCT AGAGAGCCAGCTTTAGGAGTTTTAAAGACAGTACGATCATAGAGCAAGGTTGCACCTCTGTATGTTGGGA GATGATGATGTCCATTGCTGTGTGATGGCCTGGAATTTAATTTATTTAATTAAAATCAAATTGGAGTTTA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810220 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810220 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7714 same embryo
	EMAGE:7713 same embryo
	EMAGE:7712 same embryo
	EMAGE:7715 same embryo
	EMAGE:7711 same embryo
	EurExpress:euxassay_007479 same experiment
	MGI:4823552 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source