EMAGE mouse gene expression spatial database

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EMAGE:10778

Add3 adducin 3 (gamma) ( MGI:1351615)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000998. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000998_01	euxassay_000998_02	euxassay_000998_03	euxassay_000998_04

euxassay_000998_05	euxassay_000998_06	euxassay_000998_07	euxassay_000998_08	euxassay_000998_09

euxassay_000998_10	euxassay_000998_11	euxassay_000998_12	euxassay_000998_13	euxassay_000998_14

euxassay_000998_15	euxassay_000998_16	euxassay_000998_17	euxassay_000998_18	euxassay_000998_19

euxassay_000998_20	euxassay_000998_21	euxassay_000998_22	euxassay_000998_23	euxassay_000998_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10778_wholemount_strong.wlz

10778_wholemount_moderate.wlz

10778_wholemount_weak.wlz

10778_wholemount_possible.wlz

10778_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10778_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pancreas

weak

regional

weak expression: see section 09 10 11 12 13 15 16

hypothalamus ventricular layer

weak

regional

weak expression: see section 12 13 14 15

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 12 13 14 15

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate ventricular layer

weak

regional

weak expression: see section 09 10 15 16

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 16 17

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 10 11 12 13 14

metencephalon rest of alar plate ventricular layer

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 18 19 20 21 22 23

pons mantle layer

weak

regional

weak expression: see section 08 09 17 18 19 20

pons ventricular layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17

ventral grey horn

moderate

regional

moderate expression: see section 11 12 14 15 16

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 14

not examined

regional

not examined expression: see section 02 03 04 05

stomach fundus mucosa

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11

chondrocranium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 24

foregut-midgut junction lumen

moderate

regional

moderate expression: see section 16 17

midgut loop lumen

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4296
Entity Detected:	Add3, adducin 3 (gamma) ( MGI:1351615)
Sequence:	sense strand is shown >T4296 CCACGCGTCCGGGGATGAGCTACATAAACATGTGGTGAGTTGGTGAAAACAGAAGACTAAAATAGGGCAC AGTGGCTGGAAAAGAAAGAATCCAGAGAGGAAGACGACTGGAGACATGAGTTCCGACACCAGCCCAGCTG TGGTTACCACGCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGGTATTTTGACCGAATCAATGAAAGTGA TCCGGAATACCTTCGAGAGAGGAACATGTCTCCTGATCTACGACAGGACTTCAATATGATGGAGCAGAGG AAGCGAGTCACTCAGATCCTGCAGAGCCCTGCCTTTCGGGAAGACCTGGAATGCCTCATCCAAGAGCAGA TGAAGAAAGGCCACAACCCAAGCGGCTTATTAGCGTTACAGCAGATTGCGGATTACATCGTGACCAGCTC CTTCTCGGGCTTCTCCTCGCCCTCCCTGAGTCTTGGCATGGTCACACCTATTAATGACCTTCCTGGTGCA GATACATCCTCGTATGTGAAGGGAGAAAAGCTTACTCGCTGCAAACTTGCCAGCCTCTACAGGCTTGCAG ACTTGTTTGGGTGGGCGCACCTGGCAAATACGTACATCTCGGTACGAATAAGTAAGGAGCAAGACCACAT TATAATAATTCCCAGAGGCCTCTCTTTTTCTGAAGCGACAGCCTCCACTTTGGTGAAAGTCAACATAATC GGAGAAGTGGTGGACCAGGGGAGCACTGATCTGAAAATCGACCACACGGGCTTCAGCCCGCATGCTGCCA TCTATTCAACACGCCCCGATGTGAAGTGTGTGATCCACATCCACACCCTTGCCACAGCAGCGGTGTCCTC CATGAAGTGTGGGATCCTTCCAATTTCCCAAGAGTCTCTCATCCTGGGAGATGTCGCCTACTATGACTAC CAGGGGTCGCTTGATGAGGAGGAAGAAAGAATTGAACTTCAGAAAGTGCTGGGTCCAAGCTGTAAGGTGC TCGTCCTCAGGAACCATGGCATGGTGGCTCTTGGCGAGACCCTCGAGGAGGCTTTCCATTACATCTTTAA TGTACAGATGGCCTGTGAGATCCAGGTGCAGGCAGTAGCAGGGGCAGGCGGAGTAGACAATCTGCTCGTA CTGGATCTTCAGAAATATAAAGCTTTCACCCACGGTGTCGCCATGTCTGGAGGAGGCGGTGTAAACATGG CTTCCCATCAGAAGTGGAAGGTTGGCGAGATTGAGTTTGAAGGGCTGATGAGGACCCTGGACAACTTGGG CTACAGAACAGGCTATGCGTACAGGCATCCTCTGGTCCGAGAGAAGCCTAGGCACAAGAGTGACGTGGAG ATCCCTGCAACCGTGACTGCCTTCTCCTTTGAAGATGACAGTGCTCCACTCTCTCCCCTCAAGTTCATGG CACAGAGACAGCAGCGTGAAAAAACAAGATGGCTGAATTCACCAAATACTTACATGAAGGTGAATGTGCC GGAGGAGTCTCGGAACGGGGAAACTAGTCCCAGGACCAAAATCACGTGGATGAAAGCAGAAGACTCCTCT AAAGTCAGTAGTGGGACACCGATCAAAATTGAAGACCCCAATCAGTTTGTTCCTTTAAACACAAACCCGA CGGAGGTGCTAGAAAAGAGAAATAAGATCCGGGAACAGAATCGCTATGATCTGAAGACAGCAGGGCCGCA GTCTCAGCTGCTGGCGGGGATCGTTGTGGACAAGCCGCCTTCGACCATGCAGTTTGACGATGACGATCAG GGCCCACCAGCTCCTCCTAACCCATTCAGCCACCTCCTGGAAGGAGAACTGGAAGAGTATACGAAGACAA TCGAGAGAAAGCAGCAAGGCCTGGACGACGCTGAGCAGGGGTCACTCTCAGACGACGCTGCATCTGTTTC ACAAATTCAGTCTCAAACTCAGTCACCGCAGAGTGTCCCTGAGAGATTAGAAGAAAACCACGAGCTGTTT TCCAAGAGCTTCACCTCCATGGACGCGCCTGTCATGATCATGAACGGCAAGGACGAGATGCATGATGTCG AAGACGAGCTTGCTCAGCGAGTCAGTAGGCTGACCACAAGCACGACCATAGAGAACATCGAGATCACCAT TAAGTCCCCAGAAAGAACTGAAGAGGTCCTGTCGCCTGATGGCTCACCTTCAAAGTCGCCCTCCAAGAAA AAGAAGAAATTCCGCACGCCTTCTTTTCTCAAAAAGAACAAAAAAAAGGAGAAAGTTGAGGCCTAAGTAG TCCTTTTGTAATTTGTATATTACATTGTGGCATTGCACACTTAAATACCATATTTTAGTTAATCGTTAAT ATACTGTGGTAGATCAGATTGGGAGTATGTAGCAAACTGGACTTTAAAAAACTGGAAAGAGGTTTTACTA AAAGAAAGATATTATTGAAAACTTTCAAATTCATCTCTTTTTATATGTCCGAAAACGGCCGAGCTTTTCA GCAGTGGCTGGCTTGGCCGACTCTGCCGTCCCTGGCAGAGCGTTGTTACTGTTGCTCCTTAGCAGCTGCT GCCTGGTGCCTAGGCCTCTGCAGCACATGTGCTGGGAAGGAGCTGCCAGTGAGCACGAGACAATGCTTAC TTGTCACTAATCAGACCTTGAGTTTATCATCGTGGTTATTGACTGTATCTTTGGGTCCCCACTGTTTCCA TGGGCATTAATGGGATGTTTTAAAAGCTTCTACAAGACTCTAGAGCATTAGTATACATTGGCACATCATT TTTTAAAAAATGTTTTAAGAAAAGATTTGTCTGGGTTTTTACTCATAGTATGAAATTCCCCTACCTGAAG TAACTGTTTGCCAAAAAGTCATTTTAATAAACTATAATTTTTGAAGAACTTCCTTTTTTATTAGTTTAGA AAACCCCTTATTTTTCATAGAGGGGATTTTGTACACATAATATGAGTTATTTAGTTTAACTCTTGCAAAA AAACAATTTTTGTATGTTAAAACGTTTGTATACCTTCAGTATAAGGTGTCATGTGCATGTTAGCCAATCA GTCAGGGGTAAAGCCTGCCCGAGTGCCATCCAGGAGTCCAGGGGCGTGGCACCCAAGGGCATATCAAGGC CTCGAGTCGTATTTGAACCATATAATGTATTTTTCTCTTAGTATTGCTGATGGATGAAGAGGCTGAGGGG AAGGGAAGAGTAAGAGACAGGACAGGACTGAGTCCTGTGTCCACAGAAGAAAGAACATGGAGATCGACTT GCTGAAAATAAGGCCCAAGTTTCTAAAATCCTGTTTCCTGCCAAAATCAATGTCTTGGTTGACATTTGAA TTTTAAAAGAGTTAAAGTGTACATCCTTTTAGAAGACGGCACTAGAATAAATCAGCAGGTCTAATCCCAC CAGCGAGAAAACTACCACGTCTTGATTTTTGCAAACTTAATCTTGATTTTTATAAACTTAAATTTTCTTT TAACTTCCTTTCTACTGTAAATACAAATTTAAGCCTAGATTTAATATAGTTTTCTCCATTTCCCCAAGTA ATGTCGCAGATCAATGTAAATGGATGACCCCAATACTCCACGAGTGGAAGTAAAGCGCCCTGCGGGTGAC TTGGCACGCAAACGTGACGTGACGTTGGCCGCAGTGCTCTCTGGGCTGGCTCTGTCTGACCCTGTGATTG ATGCTGGTCCTCTTAGACCTCACTAACTACTGGAGCCTGTTAATCTCGTAGTGGAAACTTTGAGTTGCTG AATTGTGCATCTGAGGATTGTTTTATGTCCTACATCATCTGTGTTGTGCTACAACGTGTGGAGACACAGT TACCTGTGAACTCTCCTTTTCCATAACTTTTAAAGAATATATATATCTAAATGAATGCAATGTGCATAAA TATTTTTTAAACACAACAGTAAACTATTGCACCTTTCGCTAACGCTTCTCTTTACTTGCTTTGACATAAT GAATGAGCCAGTGAACTCAGTGTCCTGTGGAAAATGTATAAAAGTTATCTGATATTGCTCTTAGATGTTA TGCTAATTAATGTTAAATCACAATAAACGGTATTTTAAATATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506748 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506748 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10775 same embryo
	EMAGE:10776 same embryo
	EMAGE:10777 same embryo
	EMAGE:10779 same embryo
	EurExpress:euxassay_000998 same experiment
	MGI:4823004 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source