EMAGE mouse gene expression spatial database

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EMAGE:11326

Kcnh5 potassium voltage-gated channel, subfamily H (eag-related), member 5 ( MGI:3584508)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006564. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006564_01	euxassay_006564_02	euxassay_006564_03	euxassay_006564_04

euxassay_006564_05	euxassay_006564_06	euxassay_006564_07	euxassay_006564_08	euxassay_006564_09

euxassay_006564_10	euxassay_006564_11	euxassay_006564_12	euxassay_006564_13	euxassay_006564_14

euxassay_006564_15	euxassay_006564_16	euxassay_006564_17	euxassay_006564_18	euxassay_006564_19

euxassay_006564_20	euxassay_006564_21	euxassay_006564_22	euxassay_006564_23	euxassay_006564_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11326_wholemount_strong.wlz

11326_wholemount_moderate.wlz

11326_wholemount_weak.wlz

11326_wholemount_possible.wlz

11326_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11326_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8114
Entity Detected:	Kcnh5, potassium voltage-gated channel, subfamily H (eag-related), member 5 ( MGI:3584508)
Sequence:	sense strand is shown >T8114 GACACACTCAGGGGACCCCGCCGCCGCCGCGCCCCCTTTTCTTTCAGCACCATCTCCACTACCTTTTCCT CCTCTTCTTCCACCTTTTCTTCCTGCTTCCCCCCCTCCCCCGCCGCGGATCCTGGCCGCTGCTTTCCAGA CCCAGGATGCCGGGGGGCAAGAGAGGGCTGGTGGCTCCGCAGAACACATTTTTGGAGAACATCGTCAGGC GCTCCAGTGAATCAAGTTTCTTACTGGGAAATGCCCAAATTGTGGACTGGCCTGTAGTTTATAGTAATGA CGGTTTATGTAAACTCTCTGGATATCATCGAGCTGACGTCATGCAGAAAAGCAGCACCTGCAGTTTTATG TATGGAGAACTGACCGACAAGAAGACCATTGAGAAAGTCAGGCAGACTTTTGACAACTACGAGTCAAACT GTTTTGAAGTTCTTCTGTACAAAAAAAACCGTACCCCTGTTTGGTTTTACATGCAAATCGCACCAATAAG AAATGAACACGAGAAGGTGGTCTTGTTCCTGTGTACATTCAAGGACATTACACTGTTCAAACAGCCTATA GAGGATGACTCAACGAAAGGTTGGACGAAGTTTGCCCGATTGACACGGGCTTTGACAAATAGCCGAAGTG TTTTACAGCAGCTTACACCAATGAATAAAACAGAGACAGTCCACAAGCATTCAAGACTAGCTGAAGTGCT TCAGCTGGGATCAGACATCCTTCCTCAGTACAAACAAGAAGCGCCAAAGACGCCACCACATATCATCTTA CACTATTGTGCTTTTAAAACTACTTGGGATTGGGTGATTTTAATTCTTACCTTCTACACCGCCATCATGG TTCCTTACAATGTTTCTTTCAAAACAAAGCAGAACAATATCGCCTGGCTGGTGCTGGACAGTGTGGTGGA CGTGATTTTTCTGGTGGACATTGTTTTAAATTTTCACACGACTTTTGTGGGGCCGGGTGGAGAGGTCATT TCTGACCCGAAGCTTATAAGGATGGACTATCTGAAAACTTGGTTTGTGATTGATCTGCTGTCTTGTTTAC CTTATGACATCATCAATGCCTTTGAAAATGTGGATGAGGGAATCAGCAGTCTCTTCAGCTCTTTAAAGGT GGTGCGCCTTTTACGCCTGGGCCGTGTTGCTAGGAAACTGGACCATTACCTGGAATATGGAGCAGCGGTC CTTGTGCTCCTGGTATGTGTGTTTGGACTGGTGGCTCACTGGCTGGCTTGCATCTGGTACAGCATTGGAG ACTATGAGGTCATTGATGAAGTCACCAACACCATTCAAATAGACAGCTGGCTCTACCAGTTGGCCTTGAG CATTGGGACCCCATATCGCTACAATACGAGTGCAGGGATCTGGGAAGGAGGACCCAGCAAGGACTCCCTG TATGTGTCCTCCCTCTACTTCACCATGACGAGCCTTACAACCATAGGATTTGGAAACATAGCTCCGACCA CAGACGTGGAGAAGATGTTCTCAGTGGCCATGATGATGGTCGGCTCTCTTCTTTATGCAACTATTTTTGG AAATGTTACCACAATTTTCCAGCAAATGTATGCCAACACCAACCGTTACCATGAGATGCTGAATAATGTT CGAGATTTCCTGAAATTATATCAGGTCCCAAAAGGCCTTAGTGAACGAGTGATGGATTATATTGTCTCAA CATGGTCAATGTCAAAAGGCATCGACACAGAGAAGGTCCTCTCCATCTGTCCCAAGGACATGAGAGCTGA CATCTGTGTTCATCTGAACCGGAAGGTTTTTAATGAACATCCTGCATTCCGATTGGCCAGCGATGGATGT CTGCGTGCCTTGGCAGTGGAGTTCCAAACCATTCACTGTGCTCCTGGGGACCTGATTTACCATGCTGGAG AGAGTGTGGATGCCCTCTGCTTTGTGGTGTCCGGGTCCTTGGAAGTTATCCAGGATGAGGAGGTGGTGGC CATTTTAGGGAAAGGTGACGTGTTTGGAGACATCTTCTGGAAGGAAACCACCCTTGCTCATGCGTGTGCC AATGTCCGGGCCCTGACTTACTGTGATCTGCACATCATCAAGCGAGAAGCCTTGCTCAAGGTCCTTGACT TCTATACAGCATTTGCAAACTCATTTTCCAGGAACCTCACTCTCACTTGCAATCTAAGGAAGCGGATCAT CTTCCGTAAAATTAGTGATGTGAAGAAGGAGGAGGAGGAGCGGCTGCGGCAGAAGAATGAGGTGACTCTG AGCATCCCCGTGGACCACCCAGTGAGGAAGCTCTTCCAGAAGTTCAAACAGCAGAAAGAACTGAGGATCC AGGGCTCTGCACAGAGTGATCCTGAGAGGAGCCAGCTTCAGGTAGAGAGCCGCCCTTTGCAGAACGGAGC CTCCATCACAGGTACCAGTGTGGTCACCGTATCACAGATTACCCCCATCCAGACGTCTCTGGCGTATGTG AAAACCAGCGAGTCTCTTAAGCAGAACAACCGCGATGCCATGGAACTGAAGCCCGATGGTGGCGCTGAAC CCAAATGTCTCAAGGTCAACAGCCCCATCAGGATGAAAAACGGAAATGGGAAGGGGTGGCTGAGACTCAA GAACAACATGGGGGCTCAGGAGGAGAAAAAGGAAGACTGGAATAATGTCACCAAAGCCGAGTCGATGGGG CTCTTGTCGGAGGACCCCAAGGGCAGTGACTCAGAGAACAGTGTGACCAAGAACCCCTTAAGGAAAACGG ATTCTTGTGACAGTGGGATTACAAAAAGTGACCTTCGTTTGGATAAAGCCGGTGAGGCCCGAAGTCCTCT AGAACACAGCCCCAGCCAGGCTGATGTCAAGCATTCTTTCTACCCCATCCCCGAGCAGGCCTTACAAACC ACACTGCAGGAAGTCAAACATGAACTCAAGGAGGATATCCAGCTGCTAAGCTGCAGAATGACTGCCCTGG AAAAGCAGGTGGCAGAGATTTTAAAACTACTCTCAGAGAAAAGCGTACCCCAGACATCTTCTCCAAAACC CCAAATTCCACTCCAAGTACCTCCCCAAATACCATGTCAGGATATTTTTAGTGTCTCAAGGCCTGAGTCA CCCGAATCTGACAAAGACGAAATCAACTTTTAATATATATATACATATATATTTGTTAATATATTTAAAA CAGTATATACATCTATATACATAAGTGTGTATATATAGTATATACATATATATTTTCACTTGCTTTCAAT ATGATGAACACAATATGGATTTTGATATGTACATACTGCATGTCCAGCTGGATTCTGGCCTGCCAAAGAC AATTATTCAAAACATAGATATTGCTTGTACATTATGCAGTTGACTGCATGCACACTTTACATTTATTTAT AATCTCTATTCTGTAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30094199 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30094199 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11328 same embryo
	EMAGE:11327 same embryo
	EMAGE:11325 same embryo
	EurExpress:euxassay_006564 same experiment
	MGI:4825710 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source