EMAGE mouse gene expression spatial database

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EMAGE:14427

Gsk3b glycogen synthase kinase 3 beta ( MGI:1861437)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004227. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004227_01	euxassay_004227_02	euxassay_004227_03	euxassay_004227_04

euxassay_004227_05	euxassay_004227_06	euxassay_004227_07	euxassay_004227_08	euxassay_004227_09

euxassay_004227_10	euxassay_004227_11	euxassay_004227_12	euxassay_004227_13	euxassay_004227_14

euxassay_004227_15	euxassay_004227_16	euxassay_004227_17	euxassay_004227_18	euxassay_004227_19

euxassay_004227_20	euxassay_004227_21	euxassay_004227_22	euxassay_004227_23	euxassay_004227_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14427_wholemount_strong.wlz

14427_wholemount_moderate.wlz

14427_wholemount_weak.wlz

14427_wholemount_possible.wlz

14427_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14427_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 19 20 21

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 08 14

cervical ganglion

moderate

regional

moderate expression: see section 07 14 15 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 13 14 15

retina

moderate

regional

moderate expression: see section 24

neural retina

moderate

regional

moderate expression: see section 02 03 04 05

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7156
Entity Detected:	Gsk3b, glycogen synthase kinase 3 beta ( MGI:1861437)
Sequence:	sense strand is shown >T7156 CGCGACTCGGGAGTTTCGCCGTCCTTAACTCTTGAGGCTTTTCCCTGAGGGCCAACAGCTCTGATTGGCC ACTGTCGCTATTGTCGACTCTTCTCTCTAGCCAGCCACAGGACAAGGAGAACCCTCTTCCCTTCTGTTCG GCTACCTTCGGCATTCCTCGTTGCTGGGGCGTGGTCTCCCAGCGGCGTCTCCTCATTGGTTATCCAGGTC GCTCGGCTTTTTCCGGCAGGAGCTGCAAGCAAGCGACGTCTCTGATTGGTTCCGGCTTGGGTTTCGGCTC TACAGCCGAAGCTGGCGGGCGCGGGCTCGGCGAGCGATTCCCAGACGCCTGTAACGCGGGCGGCGGGGCG CTGGGCGGTGTAAGGGCTGGGTGGGGGAGGAAGGAGGCGGAGGACGAGTAGGAAGGGGGAGGGGGAGTGG GGAAGGGCTAGGCGGCTGCGCAGACGGCGCGCCTCGCACAGAGCAGCCCCCGACCCGGCCGAATGCGGGC TTGTGTCGCCGCCGTCGCCTGGGCCGAGTGACAAAGGAAGGAAGGCAGCAAGGAGGAGCCGGCCTTGCAG CCGCTGACAGGGCTTCGGGCTCCGGGCACAGCGCGGACACTTCCCGAGCAGCCGCTGAGCAAAGGCGAGG GGCGGAGGCCGGCCGTGCCGGGGCCACGGACGGGGGAGGGGACCCCGAGGGACGGAAGCGGTTGCCTGGT TCCCATGTCCCCGGCGTATGGGGAGCAGTCAGGGAGCCGCTGCCTGGGGTCTGAAGGGAGCTGCCTCCGC CGCCGCTGCCGCCATGGCCGCTGGATCCAGCCACCGCCTGCAGCTGCTCCTGGCGCAATGAGGAGAGGAG CCGCCGCCACCGCCCGCCGCTGACTCGCGACTGCGCTGCCCTCCGGTTCACCCGGCCGCCGCCGCCGTCA GACCGCCGGATCCCGCTGCTGCCGGAGCCGCAGCGTTTGCCGTCGCATCTCCGAGCCATCTCCTCCCTCC TTCCCATCCCCCTTCTCTTCAAGCGTGAGGCTCGTGATCCTTCCGCCGCTTCCCTTCTTCATTGACTCGG AAAAAAATCCCCGAGGAAAAATATAATACTCAGAGTACTTATTTTCAATCAAGTATTTGCCCTCGTTCAC GTGATATATATATATTTTTAAGGATTCCACTCCACCCTTTTTCTCCTCTTCCAGGAAAGGGAGGTGAAAG AATTGTATTTTTCTCCCAGCTCTAAATCATCTATGTGTTAAATATCCGTACCGATCTGTCTTGAAGAAAT ACATAGCTCCTTTTTTTTTCCTTCTTCTTAAATAGCTATACAAAAGGAGTGAAAAGCCAAGAGAACGAAG TCTTTCTTTCTCTTTCTTGCGGGAGAACTTAATGCTGCATTTATCATTAACCTAGCACCCTAACATAAAA CAAGAGGAAGAAAAGGATTAAGGAAGGAAAAGGTGATTCAAGAAGAGCCATCATGTCGGGGCGACCGAGA ACCACCTCCTTTGCGGAGAGCTGCAAGCCAGTGCAGCAGCCTTCAGCTTTTGGTAGCATGAAAGTTAGCA GAGATAAAGATGGCAGCAAGGTAACCACAGTAGTGGCAACTCCTGGCCAGGGTCCTGACAGGCCACAGGA AGTCAGTTATACAGACACGAAAGTGATTGGAAATGGATCATTTGGTGTGGTATATCAAGCCAAACTTTGT GATTCTGGAGAACTGGTTGCCATCAAGAAAGTTCTACAGGACAAGCGATTTAAGAACCGAGAGCTCCAGA TCATGAGAAAGCTAGACCACTGTAACATAGTCCGACTGCGGTATTTCTTCTACTCGAGTGGCGAGAAGAA AGATGAGGTCTACCTTAACCTGGTGCTGGACTATGTTCCGGAGACAGTGTACAGAGTCGCCAGACACTAT AGTCGAGCCAAGCAGACGCTCCCTGTGATCTATGTCAAGTTGTATATGTATCAGCTGTTCAGAAGTCTAG CCTATATCCATTCCTTTGGAATCTGCCATCGAGACATTAAACCACAGAACCTCTTGTTGGATCCTGATAC AGCTGTATTAAAACTCTGTGACTTTGGAAGTGCAAAGCAGCTGGTCCGAGGAGAGCCCAATGTTTCATAT ATCTGTTCTCGGTACTACAGGGCACCAGAGTTGATCTTTGGAGCCACTGATTACACGTCCAGTATAGATG TATGGTCTGCAGGCTGTGTGTTGGCTGAATTGTTGCTAGGACAACCAATATTTCCTGGGGACAGTGGTGT GGATCAGTTGGTGGAAATAATAAAGGTCCTAGGAACACCAACAAGGGAGCAAATTAGAGAAATGAACCCA AATTATACAGAATTCAAATTCCCTCAAATCAAGGCACATCCTTGGACAAAGGTCTTCCGGCCCCGAACTC CACCAGAGGCAATTGCACTGTGCAGCCGTCTGCTGGAGTACACACCTACCGCCCGGCTAACACCACTGGA AGCTTGTGCACATTCATTTTTCGATGAATTGCGGGACCCAAATGTCAAACTACCAAATGGGCGAGACACA CCTGCACTCTTCAACTTTACCACTCAAGAACTGTCAAGTAACCCCCCTCTGGCCACCATCCTTATCCCTC CACATGCTCGGATTCAGGCCGCTGCTTCACCGCCTGCCAACGCCACAGCAGCCTCAGATACTAATGCTGG AGACCGTGGACAGACCAATAACGCCGCTTCTGCATCAGCTTCCAACTCCACCTGAACAGCCCCCAGGAGC CAGCTGCGCGGGAAAGACCAGCACTTACTTGAGTGCCACTCAGCAACACTGGTCACGTTTGGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4022374 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4022374 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14426 same embryo
	EMAGE:14429 same embryo
	EMAGE:14425 same embryo
	EMAGE:14428 same embryo
	EMAGE:14424 same embryo
	EurExpress:euxassay_004227 same experiment
	MGI:4825253 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source