EMAGE mouse gene expression spatial database

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EMAGE:7073

Mmp16 matrix metallopeptidase 16 ( MGI:1276107)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002543. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002543_01	euxassay_002543_02	euxassay_002543_03	euxassay_002543_04

euxassay_002543_05	euxassay_002543_06	euxassay_002543_07	euxassay_002543_08	euxassay_002543_09

euxassay_002543_10	euxassay_002543_11	euxassay_002543_12	euxassay_002543_13	euxassay_002543_14

euxassay_002543_15	euxassay_002543_16	euxassay_002543_17	euxassay_002543_18	euxassay_002543_19

euxassay_002543_20	euxassay_002543_21	euxassay_002543_22	euxassay_002543_23	euxassay_002543_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7073_wholemount_strong.wlz

7073_wholemount_moderate.wlz

7073_wholemount_weak.wlz

7073_wholemount_possible.wlz

7073_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7073_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 13 14 16 17 18 19 20 21 22 23 24

body-wall mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 03 04 17 weak expression: see section 18

cochlear duct

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19 20

mandible

moderate

regional

moderate expression: see section 05 06 07 11 12 13 14 weak expression: see section 08 09 10 15 16 18

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 moderate expression: see section 07 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5040
Entity Detected:	Mmp16, matrix metallopeptidase 16 ( MGI:1276107)
Sequence:	sense strand is shown >T5040 GGCCGGGTGAGGATGGTGAACACTTGCCGCCGCTGACCCTGCCTTTCCTCTGTTTCTCCCCGGCTGCCGA GCCTGGCGCCCATCCCCATCCCTGGACGGGTGGGTGAACCTGCAAGCTTTTACCCACCCCAGGACTGGAT TTTTTTTTTTTTTTTCTTTTTAAAAGGAAACAGGGAGTGGACATTCGGAAAGAGGGAGAAAACGCAGGGG AGCTCGTCCATCCGTTGAGGCACAGTTCACTATGATCTTACTCGCATTCAGCTCTGGAAGAAGGTTGGAT TTCGTGCATCGTTCGGGTGTGTTTTTCTTGCAAACCTTGCTTTGGATTTTATGTGCTACAGTCTGCGGAA CGGAGCAGTATTTCAATGTGGAGGTTTGGTTACAAAAATACGGCTACCTTCCACCGACTGACCCCAGAAT GTCAGTGCTGCGCTCTGCAGAGACCATGCAGTCAGCTCTAGCTGCCATGCAGCAGTTCTATGGCATTAAC ATGACAGGAAAAGTGGACAGAAACACAATCGACTGGATGAAGAAGCCTCGATGCGGTGTACCAGACCAGA CAAGAGGCAGCTCTAAATTCAACATTCGTCGAAAGCGCTACGCATTAACTGGACAGAAATGGCAACACAA ACATATCACTTACAGTATAAAGAACGTAACTCCAAAAGTTGGAGATCCTGAGACCCGGAGAGCAATTCGC CGTGCCTTTGATGTGTGGCAGAATGTAACTCCTCTAACGTTTGAAGAAGTTCCCTACAGTGAACTAGAAA ACGGCAAACGTGATGTGGATATAACCATCATTTTTGCTTCTGGTTTTCATGGAGACAGTTCCCCATTTGA TGGGGAGGGAGGATTTTTGGCACATGCTTATTTCCCTGGACCCGGAATTGGAGGCGACACCCATTTTGAT TCTGATGAGCCATGGACACTGGGAAATCCCAATCATGATGGAAACGATTTATTTCTTGTAGCAGTCCATG AGCTGGGACATGCTCTAGGACTGGAACATTCCAACGACCCCACCGCTATCATGGCCCCATTTTATCAGTA CATGGAAACAGACAACTTCAAGCTTCCTAATGATGATTTACAGGGCATCCAGAAGATATATGGTCCACCT GACAAGATCCCTCCACCTACAAGACCTCTACCGACAGTGCCCCCGCACCGATCTGTTCCTCCAGCAGACC CAAGGAGACATGACAGGCCCAAACCTCCTCGGCCTCCCACTGGCAGACCCTCCTATCCAGGAGCCAAACC CAACATCTGTGATGGGAACTTCAACACTCTAGCTATTCTTCGAAGGGAGATGTTTGTTTTCAAGGACCAG TGGTTTTGGCGAGTGAGAAACAACAGGGTGATGGATGGATACCCAATGCAAATTACTTACTTCTGGCGGG GCCTTCCTCCCAGTATTGATGCAGTTTATGAAAACAGTGACGGAAATTTCGTCTTCTTTAAAGGCAACAA ATACTGGGTGTTCAAAGACACAACTCTTCAACCTGGTTACCCTCATGACTTGATTACCCTTGGGAATGGA ATCCCCCCTCATGGTATTGATTCTGCCATATGGTGGGAAGATGTTGGCAAAACCTATTTCTTCAAAGGTG ACAGGTATTGGAGATATAGTGAAGAAATGAAAACCATGGACCCTGGTTACCCCAAGCCCATCACCATCTG GAAGGGGATCCCTGAATCACCTCAGGGAGCTTTCGTCCACAAAGAAAATGGCTTTACCTATTTCTACAAA GGAAAGGAGTATTGGAAATTCAACAATCAGATACTCAAAGTAGAACCTGGTTATCCAAGATCCATCCTCA AGGACTTTATGGGCTGTGATGGACCAACAGACCGAGATAAAGAAGGACTCAGCCCACCAGATGATGTAGA CATTGTCATCAAACTGGACAACACAGCCAGCACTGTGAAAGCCATAGCTATTGTCATTCCCTGCATCTTG GCCTTATGCCTCCTTGTATTGGTTTACACTGTGTTCCAGTTCAAGAGGAAAGGAACACCCCGCCACATAC TGTACTGTAAACGCTCTATGCAAGAGTGGGTGTGATGTAGGGATTTCTTTTTTCTTTCTTTTCTGGGAGT TTGTGGTAACTTGAGATTCAAGACAAGAGCTGTTATGCTGTTTCCTAGCTAGGAGCAGGCTTGTGGCAGC CTGATGGAGGCTGACCTTCCAAACCTAGATGGTTGCTGGTCCTGCACATGAGTGGAAATATAATCATGGA GAAGCTTCCGTGATGCACAGTATCTGCTGTTCTCCAGTCCTCTGTCTTTCTTTGTCATTCAGTTCTAGGC CTTTCCTCTGCACGCTCAATGCCCAGTAAAATTTCAGGATTAACTGAAGAAGAGGAAATATAGAAGAAAA GTTTCTTCTTAATTTTTTAAAAAATACATTTCCTTCTGAAATCTGAGCCCATTTCTGGGAAAGAGAAAGA AAGAGAGAGAAAGAGAGAGAGAGAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5292478 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5292478 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7072 same embryo
	EMAGE:7077 same embryo
	EMAGE:7076 same embryo
	EMAGE:7075 same embryo
	EMAGE:7074 same embryo
	EurExpress:euxassay_002543 same experiment
	MGI:4826407 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source