EMAGE mouse gene expression spatial database

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EMAGE:7263

Col16a1 collagen, type XVI, alpha 1 ( MGI:1095396)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004359. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004359_01	euxassay_004359_02	euxassay_004359_03	euxassay_004359_04

euxassay_004359_05	euxassay_004359_06	euxassay_004359_07	euxassay_004359_08	euxassay_004359_09

euxassay_004359_10	euxassay_004359_11	euxassay_004359_12	euxassay_004359_13	euxassay_004359_14

euxassay_004359_15	euxassay_004359_16	euxassay_004359_17	euxassay_004359_18	euxassay_004359_19

euxassay_004359_20	euxassay_004359_21	euxassay_004359_22	euxassay_004359_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7263_wholemount_strong.wlz

7263_wholemount_moderate.wlz

7263_wholemount_weak.wlz

7263_wholemount_possible.wlz

7263_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7263_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5166
Entity Detected:	Col16a1, collagen, type XVI, alpha 1 ( MGI:1095396)
Sequence:	sense strand is shown >T5166 CCACGCGTCCGCAGACTGGCAGGGAGAGATCGAGGATCCTCCTGTGGGCCGTGGACCCCTGGCCCTGCTT CCCATAGCTGGCAGCCTTAGGGCTGCCAGGATTGCTCAGTGAAGGGCTCCGCGGGCCGTTGGTGCCGAGG CTGCAGCTGATCTCTCTGGACCAGCATGTTGACATCCTGGGCTCCCGGCCTGTGGGTGCTCGGGCTCTGG GCCACCTTCAGCCATGGGACAAATATAGGTGAGCGGTGCCCAACTTCACAGCAAGAAGGACTCAAGCTAG AACACAGTAGTGACCCATCAACCAACGTGACTGGCTTCAACCTAATTCGCCGACTGAACCTCATGAAGAC ATCTGCCATCAAGAAGATCCGCAACCCCAAGGGGCCACTCATCTTGAGGCTGGGGGCTGCCCCAGTGACC CAACCAACTCGACGAGTGTTCCCTCGAGGCCTCCCAGAGGAGTTTGCCCTGGTCCTGACAGTGCTTCTGA AGAAACACACCTTCCGGAACACATGGTACTTGTTCCAAGTGACTGATGCAAATGGGTACCCACAGATCTC CTTGGAAGTCAACAGCCAGGAGCGGAGTCTAGAGCTCCGGGCACAGGGCCAAGATGGTGACTTTGTGTCC TGCATCTTCCCAGTGCCCCAGCTCTTCGATCTGCGTTGGCACAAGCTGATGCTGAGCGTGGCAGGCCGTG TGGCCTCTGTGCATGTGGACTGTGTCTCAGCTTCCTCCCAGCCTCTGGGACCCCGGCAATCCATCCGGCC TGGGGGACATGTATTTCTGGGCTTGGACGCTGAGCAGGGCAAGCCGGTTTCATTTGACCTTCAGCAGGCG CACATCTACTGCGACCCAGAGCTGGTGCTGGAGGAAGGCTGCTGTGAGATCTTACCGGGAGGGTGTCCCC CAGAGACCTCCAAGTCACGCCGGGACACCCAGAGCAATGAGCTCATTGAGATCAATCCTCAGACAGAGGG CAAGGTCTACACCCGGTGCTTCTGCTTAGAGGAACCTCAGAACAGCAAGGTGGATGCGCAGCTCATGGGA AGAAACATCCAGAAAGCAGAAAGGGGGACAAAGGTCCACCAGGGGACTGGAGTTAATGAGTGCCCACCCT GTGCCCACAGTGCCCGGGAGAGCAATGTCACACTTGGTCCCTCTGGCCTCAAGGGAGGGAAAGGCGAACG AGGCCTGACTGGACCATCAGGCCCCAAGGGAGAGAAGGGAGCACGGGGCAATGACTGTGTTCGAGTGTCC TCTGATGCCCCACTTCAGTGTGCAGAAGGCCCGAAAGGAGAGAAGGGAGAATCAGGGGACTTGGGACCCC CAGGACTCCCAGGACCAACAGGCCAGAAGGGCCAGAAAGGGGAGAAGGGTGATGGAGGCCTCAAAGGACT TCCAGGAAAGCCAGGACGAGATGGTCGGCCAGGAGAGATCTGTGTCATTGGACCCAAAGGGCAGAAAGGA GACCCAGGCTTTGTTGGGCCTGAGGGACTGGCAGGAGAGCCTGGGCCACCTGGCCTCCCTGGACCACCTG GGATAGGACTGCCTGGGACTCCTGGAGATCCAGGTGGCCCACCAGGCCCTAAGGGAGAAAAGGGCAGCTC TGGAATACCAGGAAAGGAAGGCCCAGGAGGGAAGCCGGGGAAACCAGGAGTGCCAGGGACTAAGGGAGAG AAGGGTGACCCCTGTGAAGTGTGCCCAAAGCTGCCTGAAGGGTCCCAGAATTTTGTGGGGCTCCCTGGAA AACCAGGGCCTAAAGGGGAACCAGGTGATCCTGCTCCAGCCTGGGAGGGCCTAGGAACTGTGGGACTGAA AGGTGACCGGGGAGATCCAGGCATCCAAGGAATGAAAGGAGAGAAGGGGGAGCCCTGCTCATCCTGTAGT TCAGGGGTCGGAGCCCAGCATCTTGGGCCCTCTCCTGGCCATGGTTTACCTGGCCTTCCGGGCACATCTG GAATTCCTGGGCCAAGAGGCCTGAAAGGGGAGAAGGGTAGTTTTGGCGATACAGGACCGGCTGGAGTTCC GGGATCACCAGGACCAGTGGGACCAGCTGGCATCAAAGGGGCAAAGGGGGAGCCCTGTGAGCCCTGCACA GTCTTGTCTGAGCTACAAGATGGGGACACACGTGTGGTTCATCTGCCCGGCCCAGCTGGAGAGAAGGGAG AGCCTGGGTCCCCGGGCTTCGGGCTTCCTGGGAAGCAGGGTAAAGCAGGAGAGCGTGGACTCAAGGGGCA GAAGGGGGATGCTGGGAATCCAGGAGACCCTGGAACACCAGGCATCACAGGGCAGCCTGGGATATCTGGA GAGCCTGGGATTCGAGGTCCTGCAGGACCAAAAGGAGAAAAGGGTGATGGATGCACTGCCTGCCCTAGCC TGCAGGGGGCGTTGACCGATGTATCGGGACTGCCTGGGAAACCTGGCCCCAAAGGAGAGCCAGGTCCAGA AGGTGTGGGCCGTCCCGGTAAACCAGGCCAGCCTGGTCTACCAGGAGTTCAAGGGCCCCCAGGACCGAAG GGCACTCAGGGAGAGCCTGGACCTCCAGGCACGGGTGCTGAGGGACCCCAGGGGGAGCCTGGAACCCAGG GTTTGCCTGGCACTCAGGGCCTTCCTGGACCTCAGGGACCACCTGGCTCAGCTGGGGAAAAGGGTGCCCA GGGATCTCCGGGGCCCAAAGGAGCCATAGGACCCATGGGACCTCCTGGTGCTGGTGTCTCGGGGCCTCCG GGCCAAAAGGGATCACGAGGCGAGAAAGGTGAACCGGGAGAGTGTTCCTGCCCTTCTCGGGGAGAGCCCA TCTTCTCGGGCATGCCGGGTCCCCCAGGTGTCCCCGGACCACCAGGTCCCCCCGGAATGCCTGGATTGCA GGGAGTGCCTGGACACAATGGTTTGCCAGGACAGCCCGGGCTCACTGCCGAGCTGGGATCCTTACCCATC GAGAAACACCTCCTCAAGAGCATCTGTGGTGACTGTGCCCAGGGCCAGACGGCTCATCCAGCCTTCCTTC TGGAGAAAGGAGAAAAGGGGGACCAGGGCATCCCTGGCGTGCCAGGTTTCGACAACTGTGCCAGGTGCTT CATAGAGAGGGAACGCCCAAGAGCTGAGGAGGCCAGGGGAGACAACAGTGAGGGAGAACCTGGCTGTTCT GGGAGCCCTGGCCTGCCTGGTCCTCCAGGGATGCCAGGCCAAAGAGGAGAAGAGGGTCCACCTGGAATGA GGGGCTCTCCCGGTCCTCCAGGCCCTATTGGCCCCCCAGGTTTTCCTGGTGCTGTTGGCTCCCCCGGATT GCCCGTAAGAACCTAGCAGCACTCCACCCCCCTTCCTGCCAGCCAGGGGCTTCAAGGGGAGCGAGGCCTC ACGGGCCTGACTGGAGACAAAGGAGAACCGGGTCCTCCAGGACAACCTGGCTACCCAGGTGCCATGGGCC CCCCAGGACTGCCTGGTATCAAGGGAGAGCGAGGATACACTGGCCCCTCGGGAGAGAAGGGCGAATCGGG CCCACCAGGATCTGAAGGCCTTCCAGGTCCCCAAGGTCCAGCGGGTCCCCGAGGAGAGCGAGGCCCCCAG GGGAGCTCTGGAGAGAAGGGTGACCAGGGATTTCAAGGCCAGCCAGGCTTCCCAGGCCCACCGGGTCCTC CAGGATTCCCAGGCAAAGCTGGAGCTCCTGGCCCACCTGGCCCCCAAGCAGAGAAGGGCAGTGAAGGGAT TCGAGGTCCCTCAGGCTTGCCTGGGTCCCCTGGGCCACCCGGCCCTCCCGGGATTCAGGGCCCTGCTGGG CTGGACGGACTGGACGGAAAGGACGGCAAGCCTGGCCTGAGGGGGGACCCAGGTCCCGCTGGCCCTCCCG GACTCATGGGACCTCCAGGCTTCAAGGGGAAAACAGGTCATCCTGGCCTCCCTGGGCCTAAGGGTGACTG TGGAAAACCCGGTCCTCCTGGCAGCAGTGGCCGGCCAGGGGCAGAGGGTGAGCCCGGTGCCATGGGACCC CAGGGACGACCAGGACCCCCAGGGCATCTTGGGCCACAGGGTCCTCCAGGCCAGCCAGGTCCACCTGGGC TCTCCACTGTGGGCCTGAAAGGAGATCGGGGAGTCCCTGGAGAAAGGGGCTTGGCGGGCCTCCCAGGCCA GCCTGGCACACCCGGACACCCCGGCCCTCCAGGTGAGCCTGGTTCGGACGGTGCAGCTGGCAAAGAGGGA CCTCCTGGAAAGCAGGGACTCTATGGACCCCCTGGCCCAAAGGGTGACCCTGGACCTGCAGGACAGAAGG GCCAAGCAGGAGAAAAGGGAAGATCTGGCATGCCGGGGGGTCCTGGCAAGAGCGGCTCCATGGGACCTAT CGGACCACCGGGGCCTGCAGGAGAGAGAGGTCACCCTGGGTCCCCAGGGCCTGCAGGGAACCCTGGATTG CCTGGTTTGCCGGGCTCTATGGGAGACATGGTGAATTATGATGATATCAAGAGGTTTATCAGACAAGAGA TCATTAAACTGTTTGATGAGAGGATGGCTTACTATACCTCCAGGATGCAGTTCCCCATGGAGGTGGCAGC AGCTCCAGGCCGGCCAGGGCCTCCGGGGAAGGATGGTGCTCCAGGTCGGCCAGGTGCTCCAGGGTCACCC GGGCTCCCTGGTCAGATAGGCCGAGAAGGACGCCAGGGTTTGCCAGGAATGAGAGGACTGCCTGGTACCA AAGGTGAAAAGGGAGACATTGGTGTTGGCATTGCAGGAGAAAATGGTCTCCCTGGCCCTCCAGGTCCTCA AGGGCCTCCAGGTTATGGCAAGATGGGTGCAACTGGACCAATGGGCCAGCAAGGTATTCCTGGGATCCCT GGGCCCCCGGGTCCCATGGGCCAGCCAGGGAAGGCAGGCCACTGCAACCCCTCAGACTGCTTTGGGGCCA TGCCAATGGAGCAGCAGTACCCACCCATGAAGAGCATGAAGGGGCCTTTCGGTTGAAACTCCCGCCCACA CTGAGATGAAGGACTCTACTAGGAATAAATGGCCAAAGCTTACAGGACTCTGATGGGTTGTGAATGTTTG TGCTATTCCTGTGTTTGTTGTTGTTGTTGCTGTTAATATTTTTTAACAATAAAGAAAACTATTTGTAACC CCACTTCCATGGGGTTCCCCTGGTGCTGCAGCTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5346676 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5346676 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7267 same embryo
	EMAGE:7268 same embryo
	EMAGE:7266 same embryo
	EMAGE:7265 same embryo
	EMAGE:7264 same embryo
	EurExpress:euxassay_004359 same experiment
	MGI:4823980 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source