EMAGE mouse gene expression spatial database

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EMAGE:14621

Grk5 G protein-coupled receptor kinase 5 ( MGI:109161)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005509. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005509_01	euxassay_005509_02	euxassay_005509_03	euxassay_005509_04

euxassay_005509_05	euxassay_005509_06	euxassay_005509_07	euxassay_005509_08	euxassay_005509_09

euxassay_005509_10	euxassay_005509_11	euxassay_005509_12	euxassay_005509_13	euxassay_005509_14

euxassay_005509_15	euxassay_005509_16	euxassay_005509_17	euxassay_005509_18	euxassay_005509_19

euxassay_005509_20	euxassay_005509_21	euxassay_005509_22	euxassay_005509_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14621_wholemount_strong.wlz

14621_wholemount_moderate.wlz

14621_wholemount_weak.wlz

14621_wholemount_possible.wlz

14621_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14621_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

testis

moderate

regional

moderate expression: see section 04 05 06 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4919
Entity Detected:	Grk5, G protein-coupled receptor kinase 5 ( MGI:109161)
Sequence:	sense strand is shown >T4919 GTTTGGCAGCAGCGGCGGCAGCTCGAGCAGCGGCAGCGGCAGCGGCATCATCCCAGACGCTGACAGCACA GCCGGCCGGCTCCCTCGCTGACTGCCGACTGTCAATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTG CTGAAAGCCCGGGAAGGGGGTGGAGGAAAGCGCAAAGGGAAAAGCAAGAAGTGGAAGGAAATCCTGAAGT TTCCTCACATCAGCCAGTGTGAAGACCTCCGAAGGACCATAGACAGAGATTACTACAGTCTCTGTGACAA GCAACCAATTGGGAGACTGCTTTTTCGACAGTTCTGTGAAACCAGGCCTGGGCTGGAGTGCTACATTCAG TTCCTGGACTTAGTGGCAGAATATGAAATTACTCCAGATGAAAACCTTGGGGCGAAGGGGAAGGAAATAA TGACCAAGTACCTCACTCCAAAGTCCCCAGTCTTCATTGCCCAAGTTGGACAGGACCTGGTCTCCCAGAC AGAGAAGAAGCTCCTGCAGAGCCCCTGCAAAGAACTCTTCTCTGCTTGTGCTCAGTCTGTCCATGACTAC TTGACGGGAGACCCCTTCCACGAGTACCTGGATAGCATGTATTTTGACCGTTTTCTGCAGTGGAAATGGT TAGAAAGACAACCAGTGACCAAAAACACTTTCCGGCAGTACCGAGTGCTGGGCAAAGGGGGCTTTGGAGA GGTCTGTGCCTGCCAGGTTCGGGCCACTGGTAAAATGTATGCTTGTAAACGCTTAGAGAAGAAGAGGATC AAAAAGAGGAAAGGCGAATCCATGGCACTCAACGAAAAGCAGATTCTTGAGAAGGTCAACAGCCAGTTTG TGGTCAACCTGGCCTATGCCTATGAAACCAAAGATGCACTATGCCTGGTTCTGACCATTATGAATGGTGG TGACCTGAAGTTTCACATCTACAATATGGGGAATCCTGGCTTTGAGGAAGAGCGAGCCTTATTTTATGCA GCTGAGATCCTCTGTGGCCTAGAAGACTTACACCGTGAGAACACTGTCTATAGAGATCTAAAACCCGAAA ACATCTTGCTGGATGATTATGGCCACATAAGGATCTCAGACCTCGGACTGGCCGTGAAGATCCCCGAGGG AGACCTTATCCGTGGCCGGGTAGGCACTGTTGGCTACATGGCCCCAGAAGTTCTGAACAACCAGCGATAT GGACTGAGCCCTGACTACTGGGGCCTGGGCTGCCTCATCTATGAGATGATTGAAGGCCAGTCACCATTTC GAGGTCGCAAGGAGAAGGTTAAGCGGGAAGAGGTGGATCGCCGGGTGCTGGAGACTGAGGAAGTATATTC CTCCAAGTTCTCTGAAGAGGCCAAGTCCATCTGCAACATGCTGCTCACCAAAGACTCGAAGCAGAGGCTG GGCTGCCAGGAGGAGGGAGCCGCCGAGGTCAAGAGGCACCCCTTCTTCAGGAACATGAACTTTAAGCGCC TGGAGGCTGGGATGTTGGACCCTCCCTTCGTTCCAGATCCCCGGGCTGTATACTGCAAGGATGTGCTGGA CATTGAGCAGTTCTCCACTGTGAAAGGTGTCAACCTGGACCATACGGACGATGATTTTTACTCAAAGTTC TCTACAGGCTCTGTGCCAATTCCATGGCAAAATGAGATGATAGAAACAGAATGTTTCAAGGAGCTGAATG TGTTCGGACCTAACGGTACCCTCTCACCAGACCTGAACAGAAGTCAGCCTCCAGAACCGCCAAAGAAAGG GCTGTTCCACAGACTCTTCAGGCGGCAGCATCAAAGCAATTCCAAGAGTTCACCTACTCCTAAGACCAGT TGTAACCACCGAATAAATTCAAACCACATCAATTCAAACTCCACTGGAAGCAGCTAGTTTCGGCTCTGGC CTTGAAGTCAAAAGTGGAACCAGCTCAGAGCCTTCTACTTGGAAGCAGAATTTATAGCCAGGGGAGCTTC CACTGTGGCTCAGTGGCCAGCAAAGCTCCAGTGGGAACTAAGATAGGAGACCGTTCCCCCAATAACAAAC CTCCAAGTTTCTCAAAGAAATTTCCACTCAGGTCTGTTTTCCAAGGTGGCCCCAAGCTGGGGTGGACTAG AATTTTCCTTGTTGAACATTGCAATAGAAACCCAATGGGATATGACAGCTTGCACGGATTTTAATAGCAT CCTAACTAGAACTGAATTTTGTCTTTATTATTTTTAAAGGAAAGTTTTGTAAATTTCAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:5116510 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5116510 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14626 same embryo
	EMAGE:14624 same embryo
	EMAGE:14625 same embryo
	EMAGE:14623 same embryo
	EMAGE:14622 same embryo
	EurExpress:euxassay_005509 same experiment
	MGI:4825238 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source