EMAGE mouse gene expression spatial database

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EMAGE:14810

Fam3b family with sequence similarity 3, member B ( MGI:1270150)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005770. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005770_01	euxassay_005770_02	euxassay_005770_03	euxassay_005770_04

euxassay_005770_05	euxassay_005770_06	euxassay_005770_07	euxassay_005770_08	euxassay_005770_09

euxassay_005770_10	euxassay_005770_11	euxassay_005770_12	euxassay_005770_13	euxassay_005770_14

euxassay_005770_15	euxassay_005770_16	euxassay_005770_17	euxassay_005770_18	euxassay_005770_19

euxassay_005770_20	euxassay_005770_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14810_wholemount_strong.wlz

14810_wholemount_moderate.wlz

14810_wholemount_weak.wlz

14810_wholemount_possible.wlz

14810_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14810_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5556
Entity Detected:	Fam3b, family with sequence similarity 3, member B ( MGI:1270150)
Sequence:	sense strand is shown >T5556 TTGCAGCCAAGAGCTGAGTAGAAAGAGAGCAGTTTCTGGAAGATGCGTCCAGTTGCTACAGGCATCTTCA AGGCACTAGTGTTTATTTTCTCCTCCCTGTGCGCCTGGTATTCTGGGTACCTGCTCGCGGAGCTCATTCC TGACGTGCCCCTGTCCAGCACTCTCTACAACATCCGAAGCATTGGAGAGAGACCTGTTCTCAAAGCCCCA GCCCCCAAAAGACAAAAATGTGACCATTGGTCCCCATGTCCTCCTGACACCTATGCCTACCGGCTGCTCA GTGGTGGTGGCCGCGACAAGTATGCCAAGATCTGCTTTGAGGATGAAGTGCTAATAGGAGAGAAGACGGG GAATGTGGCAAGAGGGATAAACATTGCTGTCGTCAACTATGAGACAGGAAAAGTGATAGCGACAAAGTAC TTTGATATGTATGAAGGTGATAACTCCGGGCCAATGGCCAAGTTCATTCAGAGCACTCCTTCAAAATCCC TGCTGTTCATGGTGACTCATGATGATGGAAGTTCCAAACTGAAGGCTCAAGCAAAGGATGCCATAGAAGC CCTTGGAAGCAAAGAAATCAAGAACATGAAGTTCAGATCAAGCTGGGTGTTTGTTGCAGCAAAGGGCTTT GAGCTCCCTTCAGAAATCGAGAGAGAAAAAATCAACCACTCAGATCAATCCAGGAACAGATATGCAGGCT GGCCAGCGGAGATCCAGATCGAAGGATGCATACCCAAAGGGCTGAGATAACGGTGCTTGGACCTCAATAA ACACGTTTTGTACAGATATACTCAGCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6474753 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6474753 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14812 same embryo
	EMAGE:14811 same embryo
	EMAGE:14809 same embryo
	EMAGE:14808 same embryo
	EMAGE:14813 same embryo
	EurExpress:euxassay_005770 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source