Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17100

Epcam epithelial cell adhesion molecule ( MGI:106653)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100
"Pseudo-wholemount" of euxassay_008863. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008863_01 euxassay_008863_02 euxassay_008863_03 euxassay_008863_04
EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100
euxassay_008863_05 euxassay_008863_06 euxassay_008863_07 euxassay_008863_08 euxassay_008863_09
EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100
euxassay_008863_10 euxassay_008863_11 euxassay_008863_12 euxassay_008863_13 euxassay_008863_14
EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100
euxassay_008863_15 euxassay_008863_16 euxassay_008863_17 euxassay_008863_18 euxassay_008863_19
EMAGE:17100 EMAGE:17100 EMAGE:17100 EMAGE:17100
euxassay_008863_20 euxassay_008863_21 euxassay_008863_22 euxassay_008863_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17100Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17100_wholemount_strong.wlz
17100_wholemount_moderate.wlz
17100_wholemount_weak.wlz
17100_wholemount_possible.wlz
17100_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17100_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hand
strong strong
regionalstrong expression: see section 01 02 03 17 18 19 20 21 22 23
foot
strong strong
regionalstrong expression: see section 01 02 03 17 18 19
thymus primordium
strong strong
regionalstrong expression: see section 09 10 11 12 13 14
sublingual gland primordium
strong strong
regionalstrong expression: see section 08 14
submandibular gland primordium
strong strong
regionalstrong expression: see section 05 06 07 15 16 17
pancreas
strong strong
regionalstrong expression: see section 06 07 08 09 11 12 13 14
thyroid gland
strong strong
regionalstrong expression: see section 08 09 10 12 13
pituitary gland
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13
epidermis
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
vibrissa
strong strong
regionalstrong expression: see section 02 03 04 16 17 18 19 20
cochlea
strong strong
regionalstrong expression: see section 04 05 06 07 14 15 16 17
utricle
strong strong
regionalstrong expression: see section 02 03 04 17 18 19 20 21
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 21 22 23
conjunctival sac
strong strong
regionalstrong expression: see section 01 02 19 20 21 22
cornea epithelium
strong strong
regionalstrong expression: see section 01 02 19 20 21 22
anterior naris
strong strong
regionalstrong expression: see section 08 09 10 11 12 13
external naris
strong strong
regionalstrong expression: see section 07 08 09 12 13
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 05 06 07 08 09 11 12 13 14 15 16
naso-lacrimal duct
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19 20
vomeronasal organ
strong strong
regionalstrong expression: see section 09 12
esophagus epithelium
strong strong
regionalstrong expression: see section 11 12
pharynx epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13
tongue epithelium
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08
rectum
strong strong
regionalstrong expression: see section 10
midgut
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16
lower jaw incisor
strong strong
regionalstrong expression: see section 08 09 12 13 14
lower jaw molar
strong strong
regionalstrong expression: see section 04 05 07 14 16
oral epithelium
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
upper jaw incisor
strong strong
regionalstrong expression: see section 08 09 12 13
upper jaw molar
strong strong
regionalstrong expression: see section 04 17
urinary system
strong strong
regionalstrong expression: see section 03 04 18 19
bladder
strong strong
regionalstrong expression: see section 10 11
renal cortex
strong strong
regionalstrong expression: see section 05 06 07 08 13 14 15 16 17
male reproductive system
strong strong
regionalstrong expression: see section 04 05 10 11 12 13 14 15 16 17 18
seminiferous cord
strong strong
regionalstrong expression: see section 05 16 17 18
urethra of male
strong strong
regionalstrong expression: see section 10 11
larynx
strong strong
regionalstrong expression: see section 10 11 12
left lung
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11
right lung
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19 20 21
main bronchus epithelium
strong strong
regionalstrong expression: see section 08 09 10 13
trachea epithelium
strong strong
regionalstrong expression: see section 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4479
Entity Detected:Epcam, epithelial cell adhesion molecule ( MGI:106653)
Sequence:sense strand is shown

>T4479
GTCCGCTCCCGCCTCGCCGCGCGCACAGCGCTCAGTCCGTCCGCCGCCGCGCAGCGCGACTGTCCTCCGA
GCCGTCCCGCGCCGCACCTCCGCGAGTCGCCCTCGCCGCTCCGCGCGCAGCATGGCGGGTCCCCAGGCCC
TCGCGTTCGGGCTCCTGCTCGCGGTGGTCACAGCGACGCTGGCCGCGGCTCAGAGAGACTGTGTCTGTGA
CAACTACAAGCTGGCAACAAGTTGCTCTCTGAATGAATATGGTGAATGCCAGTGTACTTCCTATGGTACA
CAGAATACTGTCATTTGCTCCAAACTGGCGTCTAAATGCTTGGCGATGAAAGCAGAAATGACTCACAGCA
AGTCTGGGAGGAGGATAAAGCCCGAAGGGGCGATCCAGAACAACGATGGGCTGTACGACCCCGACTGCGA
CGAGCAGGGGCTCTTCAAAGCCAAGCAGTGCAACGGCACCGCCACGTGCTGGTGTGTCAACACCGCCGGA
GTCCGAAGAACCGACAAGGACACGGAGATCACGTGCTCCGAGCGCGTGAGGACCTACTGGATCATCATTG
AACTAAAACACAAAGAAAGAGAAAGCCCCTACGACCATCAGAGCTTGCAGACTGCGCTTCAAGAGGCGTT
CACATCTCGATATAAGCTGAATCAGAAATTTATCAAAAACATTATGTATGAGAATAATGTTATCACCATT
GATCTGATGCAAAACTCTTCTCAGAAAACACAAGACGACGTGGACATAGCTGATGTGGCTTACTATTTTG
AAAAAGATGTGAAGGGGGAGTCCCTGTTCCATTCTTCTAAGAGCATGGACCTGAGAGTGAACGGAGAGCC
GCTCGATCTGGACCCCGGGCAGACTCTGATTTACTACGTTGATGAAAAGGCACCCGAGTTCTCCATGCAG
GGCCTCACGGCCGGGATCATCGCTGTCATTGTGGTGGTGTCATTAGCAGTCATCGCGGGGATTGTTGTCC
TGGTTATATCTACAAGGAAGAAATCAGCAAAATATGAGAAGGCTGAGATAAAGGAGATGGGTGAGATCCA
CAGAGAGCTTAATGCCTAGCCGTGCTGAGTGCTGAACTGAGGAGGGGCCGCCCGACCGGAAGTGGCAGAA
GAGCTCGGACTGCAGATGTATAAACCTGGGGAAGATGAAGACCTGCGAAGGGTTACTGCTTTGATAGTTA
CTTTGTTAGTTTCACATTTGTAACAGTGAAATTTGTACTCGTAAATACAAGCAGCTGGACACCGGCATTA
CCGATCGTAAAATTAGACGAACGTCTTATAGGTGCAGGTCCAGTGTGGTACTCAGAACTTAGCCTGCAAA
GTTAAGAGAGTTGATGCTTATTATGACAGAGTGTGCGTCGCAAACATTCCAACAGTAGAATGCGGTGACT
AGTCTCATTTTTTTTTTTTTTTTGTGATTAAGGCTGCCCTTCTATATACCTGAGTCTTGTACATAATAAA
CTTTTTTTTAATGAAATAAAACATTTTAAAGTGAGTTTCTAAGTTTGTTTGAATCAAATTTTCCTAGCAT
GTGCATAATTAAGATAATAGATGTCTAAATGCTCTGGCACTGCTAACTGGTACAAACCTGTAATTCTGTA
CTTGGGAGGTAGAGGTAGGAGGGTTAGCGCTTCCGAGGTAGCTGCTGTGTATCTGCTCTGCCACTGACTG
GCCTTGACTATCCAACACCCTATCTGAAAGAAATAAAAATCAAACTTAAAAAAAAAAAAAAAAAAAAAAA
A
Notes:The probe template was PCR amplified from IMAGE:4948935 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4948935 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17105 same embryo
 EMAGE:17101 same embryo
 EMAGE:17103 same embryo
 EMAGE:17102 same embryo
 EMAGE:17104 same embryo
 EurExpress:euxassay_008863 same experiment
 MGI:4824577 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS