EMAGE mouse gene expression spatial database

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EMAGE:17103

Mecp2 methyl CpG binding protein 2 ( MGI:99918)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001493. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001493_01	euxassay_001493_02	euxassay_001493_03	euxassay_001493_04

euxassay_001493_05	euxassay_001493_06	euxassay_001493_07	euxassay_001493_08	euxassay_001493_09

euxassay_001493_10	euxassay_001493_11	euxassay_001493_12	euxassay_001493_13	euxassay_001493_14

euxassay_001493_15	euxassay_001493_16	euxassay_001493_17	euxassay_001493_18	euxassay_001493_19

euxassay_001493_20	euxassay_001493_21	euxassay_001493_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17103_wholemount_strong.wlz

17103_wholemount_moderate.wlz

17103_wholemount_weak.wlz

17103_wholemount_possible.wlz

17103_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17103_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

tail dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4478
Entity Detected:	Mecp2, methyl CpG binding protein 2 ( MGI:99918)
Sequence:	sense strand is shown >T4478 GGGCTGTGGTAAAACCCGTCCGGAAAATGGCCGCCGCTGCCGCCACCGCCGCCGCCGCCGCCGCGCCGAG CGGAGGAGGAGGAGGAGGCGAGGAGGAGAGACTGCTCCATAAAAATACAGACTCACCAGTTCCTGCTTTG ATGTGACATGTGACTCCCCAGAATACACCTTGCTTCTGTAGACCAGCTCCAACAGGATTCCATGGTAGCT GGGATGTTAGGGCTCAGGGAGGAAAAGTCAGAAGACCAGGATCTCCAGGGCCTCAGAGACAAGCCACTGA AGTTTAAGAAGGCGAAGAAAGACAAGAAGGAGGACAAAGAAGGCAAGCATGAGCCACTACAACCTTCAGC CCACCATTCTGCAGAGCCAGCAGAGGCAGGCAAAGCAGAAACATCAGAAAGCTCAGGCTCTGCCCCAGCA GTGCCAGAAGCCTCGGCTTCCCCCAAACAGCGGCGCTCCATTATCCGTGACCGGGGACCTATGTATGATG ACCCCACCTTGCCTGAAGGTTGGACACGAAAGCTTAAACAAAGGAAGTCTGGCCGATCTGCTGGAAAGTA TGATGTATATTTGATCAATCCCCAGGGAAAAGCTTTTCGCTCTAAAGTAGAATTGATTGCATACTTTGAA AAGGTGGGAGACACCTCCTTGGACCCTAATGATTTTGACTTCACGGTAACTGGGAGAGGGAGCCCCTCCA GGAGAGAGCAGAAACCACCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGGGGTCGGGGACG CCCCAAAGGGAGCGGCACTGGGAGACCAAAGGCAGCAGCATCAGAAGGTGTTCAGGTGAAAAGGGTCCTG GAGAAGAGCCCTGGGAAACTTGTTGTCAAGATGCCTTTCCAAGCATCGCCTGGGGGTAAGGGTGAGGGAG GTGGGGCTACCACATCTGCCCAGGTCATGGTGATCAAACGCCCTGGCAGAAAGCGAAAAGCTGAAGCTGA CCCCCAGGCCATTCCTAAGAAACGGGGTAGAAAGCCTGGGAGTGTGGTGGCAGCTGCTGCAGCTGAGGCC AAAAAGAAAGCCGTGAAGGAGTCTTCCATACGGTCTGTGCATGAGACTGTGCTCCCCATCAAGAAGCGCA AGACCCGGGAGACGGTCAGCATCGAGGTCAAGGAAGTGGTGAAGCCCCTGCTGGTGTCCACCCTTGGTGA GAAAAGCGGGAAGGGACTGAAGACCTGCAAGAGCCCTGGGCGTAAAAGCAAGGAGAGCAGCCCCAAGGGG CGCAGCAGCAGTGCCTCCTCCCCACCTAAGAAGGAGCACCATCATCACCACCATCACTCAGAGTCCACAA AGGCCCCCATGCCACTGCTCCCATCCCCACCCCCACCTGAGCCTGAGAGCTCTGAGGACCCCATCAGCCC CCCTGAGCCTCAGGACTTGAGCAGCAGCATCTGCAAAGAAGAGAAGATGCCCCGAGGAGGCTCACTGGAA AGCGATGGCTGCCCCAAGGAGCCAGCTAAGACTCAGCCTATGGTCGCCACCACTACCACAGTTGCAGAAA AGTACAAACACCGAGGGGAGGGAGAGCGCAAAGACATTGTTTCATCTTCCATGCCAAGGCCAAACAGAGA GGAGCCTGTGGACAGCCGGACGCCCGTGACCGAGAGAGTTAGCTGACTTTACATAGAGCGGATTGCAAAG CAAACCAACAAGAATAAAGGCAGCTGTTGTCTCTTCTCCTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4948925 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4948925 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17105 same embryo
	EMAGE:17101 same embryo
	EMAGE:17102 same embryo
	EMAGE:17104 same embryo
	EMAGE:17100 same embryo
	EurExpress:euxassay_001493 same experiment
	MGI:4826148 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source