EMAGE mouse gene expression spatial database

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EMAGE:17263

Uqcrc2 ubiquinol cytochrome c reductase core protein 2 ( MGI:1914253)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001653_01	euxassay_001653_02	euxassay_001653_03	euxassay_001653_04	euxassay_001653_05

euxassay_001653_06	euxassay_001653_07	euxassay_001653_08	euxassay_001653_09	euxassay_001653_10

euxassay_001653_11	euxassay_001653_12	euxassay_001653_13	euxassay_001653_14	euxassay_001653_15

euxassay_001653_16	euxassay_001653_17	euxassay_001653_18	euxassay_001653_19	euxassay_001653_20

euxassay_001653_21	euxassay_001653_22	euxassay_001653_23	euxassay_001653_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17263_wholemount_strong.wlz

17263_wholemount_moderate.wlz

17263_wholemount_weak.wlz

17263_wholemount_possible.wlz

17263_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17263_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5674
Entity Detected:	Uqcrc2, ubiquinol cytochrome c reductase core protein 2 ( MGI:1914253)
Sequence:	sense strand is shown >T5674 CGGACGCGTGGGCGACCCCATCTTGCTTTGCTGTCTGCAGCGGTGACCGTGGGAAAGGGCAACTGCTAGA GCCATGAAGCTCCTCAGCAGGGCCGGGTCCTTCTCGAGATTTTATTCCCTCAAAGTTGCCCCGAAGGTTA AAACTTCAGCAGCCCCTGGAGGAGTGCCTCTGCAGCCTCAGGATCTTGAGTTTACCAAATTACCAAATGG TTTGGTGATTGCTTCTCTGGAAAACTATGCTCCTCTGTCAAGAATCGGCTTGTTCGTTAAAGCAGGCAGT AGATATGAGGACTCCAACAACTTGGGAACCTCACATTTACTACGTCTTGCATCTAGTTTGACTACCAAAG GAGCTTCATCTTTCAAGATAACCCGTGGGATTGAAGCAGTTGGTGGTAAATTAAGTGTGACTGCAACAAG GGAAAACATGGCGTACACTGTAGAAGGCATTCGGAGTGATATTGAGATCCTCATGGAGTTCCTGCTCAAT GTCACCACAGCACCCGAGTTTCGCCGTTGGGAAGTAGCTGCCCTGCGGTCTCAGCTAAAGATTGACAAAG CTGTTGCCTTTCAGAATTCACAGACCCGTATCATTGAGAACTTGCATGATGTTGCCTACAAGAATGCCTT GGCTAATCCTTTGTACTGTCCTGACTATAGGATGGGGAAGATAACATCAGAGGAATTACATTACTTTGTT CAGAACCACTTCACAAGTGCAAGAATGGCTCTGGTTGGACTTGGTGTGAGTCATTCTGTCTTAAAGCAGG TGGCTGAACAATTTCTCAACATGAGGGGTGGCCTTGGTTTAGCTGGTGCAAAAGCCAAATACCGTGGAGG TGAAATTAGAGAGCAGAATGGAGACAACCTGGTCCATGCTGCTATTGTTGCAGAAAGTGCTGCCATTGGA AATGCAGAGGCAAATGCATTCAGTGTACTTCAGCATCTTCTTGGTGCTGGACCACATATCAAAAGGGGCA ACAACACCACCAGCCTCCTGAGCCAGAGTGTTGCCAAAGGAAGTCACCAGCCTTTTGATGTTTCTGCATT TAATGCCAGTTACTCAGACTCTGGGCTCTTTGGAATTTACACTATTTCCCAGGCTGCAGCTGCTGGAGAG GTTATCAATGCTGCCTACAACCAAGTCAAAGCAGTTGCTCAAGGAAACCTTTCCAGTGCAGATGTCCAAG CTGCCAAGAACAAGCTAAAAGCTGGATATCTAATGTCTGTGGAGACGTCAGAGGGCTTCCTGAGTGAAAT TGGGTCACAGGCTCTAGCTGCAGGTTCTTATATGCCACCTTCTACCGTCCTTCAACAGATTGACTCCGTG GCTGATGCTGATGTTGTAAAGGCTGCAAAAAAGTTTGTTTCTGGCAAGAAGTCAATGGCAGCGAGTGGAA ACTTGGGACATACGCCTTTTCTCGACGAGTTATAACACATGCGTGTATTTAAAGCTCAGCCCAGACCAGC AAGCACATCAAAGTCAGGTCTCTTAATAATTAATTAATGTTAATTCAACATTTTCTCAATGAAGTAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2609295 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2609295 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17258 same embryo
	EMAGE:17262 same embryo
	EMAGE:17259 same embryo
	EMAGE:17264 same embryo
	EMAGE:17260 same embryo
	EMAGE:17261 same embryo
	EurExpress:euxassay_001653 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source