EMAGE mouse gene expression spatial database

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EMAGE:17309

Psmd6 proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 ( MGI:1913663)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003728. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003728_01	euxassay_003728_02	euxassay_003728_03	euxassay_003728_04

euxassay_003728_05	euxassay_003728_06	euxassay_003728_07	euxassay_003728_08	euxassay_003728_09

euxassay_003728_10	euxassay_003728_11	euxassay_003728_12	euxassay_003728_13	euxassay_003728_14

euxassay_003728_15	euxassay_003728_16	euxassay_003728_17	euxassay_003728_18	euxassay_003728_19

euxassay_003728_20	euxassay_003728_21	euxassay_003728_22	euxassay_003728_23	euxassay_003728_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17309_wholemount_strong.wlz

17309_wholemount_moderate.wlz

17309_wholemount_weak.wlz

17309_wholemount_possible.wlz

17309_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17309_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5695
Entity Detected:	Psmd6, proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 ( MGI:1913663)
Sequence:	sense strand is shown >T5695 GCCGGCTCCTGTGCTGTAGCCATGCCGCTAGAAAACCTAGAAGAAGAGGGACTGCCCAAGAACCCCGACC TGCGCATCGCGCAGCTGCGGTTTCTGCTTAGCCTGCCGGAGCACCGCGGGGACGCGGCGGTGCGCGAGGA GTTGATGGCGGCCGTCCGGGAAAACAATATGGCTCCTTATTATGAAGCCTTGTGTAAATCCCTCGACTGG CAGATGGACGTGGATCTCCTGAGTAAAATGAAGAAAGCAAACGAAGAAGAGTTGAAACGTTTGGATGAGG AGCTGGAGGATGCAGAGAAGAATCTGGGAGAAAGTGAAATTCGAGATGCAATGATGGCAAAAGCAGAGTA CCTCTGTCAGATAGGTGACAAGGAAGGAGCTTTGACAGCTTTTCGCAAGACATACGATAAGACTGTGGCC CTGGGTCACCGCCTGGATATTGTATTCTATCTCCTAAGGATCGGACTCTTTTATATGGATAATGACCTCA TTACTCGAAACACAGAAAAGGCCAAAAGCCTGATAGAGGAAGGCGGAGACTGGGACAGGCGAAACAGGCT GAAAGTGTACCAGGGCTTATATTGTGTGGCTATCCGTGACTTCAAACAGGCAGCAGAGCTCTTTCTCGAC ACTGTTTCTACGTTTACATCGTATGAGCTCATGGATTACAAGACATTTGTGACTTACACTGTTTACGTCA GTATGATCGCCTTAGAAAGGCCGGACCTCAGGGAAAAGGTCATTAAAGGGGCAGAGATCCTTGAAGTGTT GCACAGTCTTCCAGCAGTTCGGCAGTATCTGTTTTCTCTCTATGAATGCCGTTATTCAGTTTTTTTCCAA TCATTAGCAATCGTGGAACAGGAAATGAAAAAGGATTGGCTTTTTGCGCCTCATTACCGATACTATGTAA GAGAAATGAGAATCCATGCGTACAGCCAGCTGCTGGAATCATATAGGTCATTAACCCTCGGCTATATGGC AGAAGCTTTTGGTGTGGGTGTGGACTTCATTGACCAGGAGCTGTCCAGGTTTATTGCTGCTGGAAGACTC CACTGCAAAATTGATAAAGTAAATGAAATCGTGGAGACCAACAGACCTGATAGCAAGAACTGGCAGTATC AAGAAACCATCAAGAAAGGAGACTTGCTGCTAAACAGGGTTCAGAAATTATCCAGAGTAATTAACATGTA AAGCCACTTTTCAACGTGTTCCTTTACAGATGTTACTTGGAAGCTTTTCTAACTTCTTCACTCTGTGCCC ACTTCAACAGCATCAAATAAATATGTCATTTGTTTCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2646325 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2646325 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17311 same embryo
	EMAGE:17310 same embryo
	EMAGE:17307 same embryo
	EMAGE:17308 same embryo
	EMAGE:17312 same embryo
	EurExpress:euxassay_003728 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source