EMAGE mouse gene expression spatial database

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EMAGE:17623

E430025E21Rik RIKEN cDNA E430025E21 gene ( MGI:2146110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005959. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005959_01	euxassay_005959_02	euxassay_005959_03	euxassay_005959_04

euxassay_005959_05	euxassay_005959_06	euxassay_005959_07	euxassay_005959_08	euxassay_005959_09

euxassay_005959_10	euxassay_005959_11	euxassay_005959_12	euxassay_005959_13	euxassay_005959_14

euxassay_005959_15	euxassay_005959_16	euxassay_005959_17	euxassay_005959_18	euxassay_005959_19

euxassay_005959_20	euxassay_005959_21	euxassay_005959_22	euxassay_005959_23	euxassay_005959_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17623_wholemount_strong.wlz

17623_wholemount_moderate.wlz

17623_wholemount_weak.wlz

17623_wholemount_possible.wlz

17623_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17623_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 04 05 06 22 23

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall marginal layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon

strong

regional

strong expression: see section 01 24

cerebral cortex mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 not examined expression: see section 01

cerebral cortex marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 not examined expression: see section 01

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

telencephalon marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

medulla oblongata alar plate marginal layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate marginal layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

rest of cerebellum marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

pons marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

strong

regional

strong expression: see section 04 05 19

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain marginal layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 19 20 21

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 05 06 07 17 18 19

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

homogeneous

strong expression: see section 07 17

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 05 06 07 08 17 18

trigeminal v nerve

strong

homogeneous

strong expression: see section 09 17

spinal cord

strong

regional

strong expression: see section 07

spinal cord mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

spinal cord marginal layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

strong

regional

strong expression: see section 08 09 moderate expression: see section 12 13 14 15

cervical ganglion

strong

homogeneous

strong expression: see section 07 08 16 17

thoracic ganglion

strong

regional

strong expression: see section 09 moderate expression: see section 10 11

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 12 13 14 15 16 17

neural retina

strong

regional

strong expression: see section 23 24 moderate expression: see section 01 02 03 04

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 moderate expression: see section 09 11 12 14 15 16 17 18

nasal septum

moderate

regional

moderate expression: see section 12 15 16

tongue muscle

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 10 11 12 16

stomach

moderate

regional

moderate expression: see section 01 02 03 04 06 07 08 09 weak expression: see section 05

genital tubercle of male

moderate

regional

moderate expression: see section 09 10 11 12 13

testis

moderate

regional

moderate expression: see section 09 weak expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9517
Entity Detected:	E430025E21Rik, RIKEN cDNA E430025E21 gene ( MGI:2146110)
Sequence:	sense strand is shown >T9517 CCCGGGTCCCGGCAGCTGACGCTCTGCGGCCCGGGACTCTAGGGGGATCCCTGGGGTGGGAGACGGGGAC CGGGGCGCCCAGTCCACGCGGCCAGCGTCGGACGGCTGACAGGTTCTTTAATGGAGCGGCCATTTGTCCT ACAGATCTGGCTTCATGTCATGATCCACCAACCCCTGCTCTGAAGTCAGTCATTCCCACCCTTCAGGTGT GGACTAGCCAGTCACTCTCACCATGTTGGACTTCCTGGCTGAGAATAATCTCTGTGGCCAGGCCATCCTG AGGATCGTCTCCTGTGGCAATGCCATCATTGCCGAGGTGCTGCGGCTCTCTGAGTTCATTCCTGCTGTGT TCCTGCTGAAAGACCGGGCTGACCAGCAGAGATATGGAGACATCATCTTTGACTTCAGCTACTTTAAGGG TCCAGAGTTCTGGGAAAGCAAACTGGAAGCCAAGCCTGAGCTGCAGGATTTAGATGAAGAATTTCGGGAA AACAACATAGAAATCGTGACCAGATTTTATTTAGCCTTTCAAAGTGTGCATAAATACATTGTAGACTTAA ACAGATACCTCGATGACCTCAATGAAGGAGTTTATATTCAGCAAACCTTAGAAACCGTGCTTCTCAGTGA AGATGGAAAACAACTTCTATGTGAAGCCCTGTACTTATATGGAGTTATGCTGCTTGTCATCGACCAGAAG ATTGAAGGAGAAGTCAGAGAGAGAATGCTGGTTTCTTACTATCGATACAGTGCTGCTCGGTCCTCTGCTG ACTCAAACATGGATGATATCTGTAAGCTGCTACGAAGCACAGGATATTCCAGCCAGCCAGGAGCCAAAAG ACCACCCAACTATCCTGAGAGCTATTTCCAGAGAGTGCCTATCAACGAGACGTTCATCAGCATGGTCATC GGACGCCTGAGGTCTGATGACATTTACAACCAGGTCTCTGCCTACCCGCTGCCGGAGCACCGCAGCACTG CCCTGGCCAATCAAGCCGCCATGCTCTACGTGATTCTCTATTTTGAACCTTCCATTCTTCACACCCACCA AGCAAAAATGAGAGAGATCGTGGATAAGTACTTTCCAGATAACTGGGTAATCAGCATCTATATGGGTATC ACTGTTAATCTAGCTGATGCCTGGGAACCTTACAAAGCTGCGAAAACTGCTCTCAACAACACCCTTGACC TCGCCAATGTCAAAGAACAGGCAAGCAGATACGCTAGTGTCAGCGACAGAGTGCGCGCTCAGGTGCAGCA GTTTCTGAAAGAGGGCTACCTGAGGGAGGAGGTGCTTCTGGACAACATCCCCAGGCTGCTGAACTGCCTG AGAGACTGCAATGTCGCCATCCGCTGGCTAATGCTCCACACAGCCGACTCAGCCTGTGACCCAAACAACA AGCGCCTTCGTCAAATCAAGGACCAGATTCTGGCAGACTCTAGGTACAATCCCAAGATCCTCTTCCAGTT GCTATTGGACACTGCACAATTCGAGTTTATACTCAAAGAGATGTTCAAGCAAATGCTTTCCGAGAAACAG TCCAAGTGGGAGCATTACAAGAAGGAGGGCTCTGAGCGCATGACCGAGCTTGCTGACGTCTTTTCAGGGG TGAAACCACTAACCCGAGTGGAGAAAAACGAAAATCTTCAAGCTTGGTTCAGAGAGATCTCAAAACAAAT ACTGTCTCTAAATTATGATGATTCTACTGCTGCAGGCAGAAAAACTGTGCAACTAATACAAGCTCTGGAA GAGGTTCAGGAGTTTCACCAGTTGGAATCAAATCTACAAGTTTGCCAGTTTCTAGCTGACACCCGAAAGT TTCTCCATCAAATGATCAGAACCATCAACATCAAGGAGGAAGTCCTGATCACAGTGCAGATCATCGGGGA CCTTTCCTTTGCCTGGCAGTTAATTGACAGTTTCACATCCATCATGCAAGAAAGCATAAGGGTAAACCCA TCCATGGTTACCAAACTCAGAGCCACGTTCCTAAAGCTTGCCTCTGCCCTTGACCTTCCCCTGCTACGTA TTAATCAAGCAAATAGCCCTGATCTCCTCAGTGTGTCTCAGTATTATTCAGGAGAATTGGTGTCATACGT GAGAAAGGTTTTGCAGATCATTCCAGAAAGCATGTTCACATCTCTCCTGAAGATCATAAAGCTTCAGACC CATGACATCATGGAAGTGCCTACACGCCTGGACAAGGACAAGCTGCGGGACTACGCTCAGCTTGGCCCGA GATATGAGGTTGCCAAGCTCACTCACGCCATCTCCATCTTTACTGAGGGCATCTTGATGATGAAAACGAC CCTGGTGGGCATCATCAAGGTGGATCCCAAGCAGTTGCTGGAAGACGGAATAAGGAAAGAGCTGGTTAAA CGCGTGGCTTTCGCCCTTCACCGGGGACTGATATTCAACCCTCGTGCCAAGCCAAGTGAGTTGATGCCCA AACTGAAAGAGCTGGGGGCCACCATGGATGGGTTCCATCGCTCCTTTGAGTATATACAGGACTACGTCAG CATTTACGGTCTGAAGATTTGGCAGGAGGAAGTCTCTCGTATTATAAATTATAACGTGGAACAGGAGTGT AATAACTTCTTAAGAACAAAGATTCAAGACTGGCAGAGTATGTACCAATCCACTCATATACCAATCCCGA AGTTTGCACCTGTGGATGAGTCTATAACATTTATTGGTCGACTCTGCAGAGAAATCTTGCGGATCACAGA CCCAAAAATGACATGTTACATAGACCAGCTGAACACTTGGTATGACGTGAAAACTCATCAAGAAGTGACC AGCAGTCGCCTCTTCTCAGAAATCCAGACCACCTTGGGGACTTTTGGTCTGAATGGATTGGACAGGCTTC TGTGCTTCATGATTGTGAAAGAGCTACAGAATTTCCTCAGTATGTTTCAGAAAATTATCCTGAAAGAGAG AACTGTTCAAGAAACTTTAAAGATGCTCATGAGTGCTGTTAACCCTCTAAAGAGCATAGTGGCAAATTCA AGTAAAGTTTATCTTTCTGCCATCACCAAAACACAGAAGATCTGGAGTGCTTACCTGGAGGCTATAATGA AGGTTGGACAGATGCAGATCCTGAGACAACAGATCGCCAATGAGCTAAACTCTTCCTGTCGATTTGATTC CAGACATCTGGCAGCTGCCCTGGATAATCTCAATAAGGCTCTCCTGGCAGACATCGAAGCCCACTACCGG GACCCTTCGCTTCCTTACCCCAAAGAAGATAACACACTCTTATATGAAATCACAGCCTATCTGGAGGCGG CTGGCATTCACAACCCCCTGAATAAGATTTACATAACAACGAAGCGCTTACCCTATTTCCCGATTGTCAA CTTTCTGTTTTTGATCGCGCAGTTGCCAAAACTTCAGTATAACAAAAATCTAGGCATGGTCTGCCGGAAG CCTGCCGACCCTGTTGACTGGCCACCGCTCGTCCTAGGTCTGCTCACGCTGCTGAAGCAGTTCCACTCCC GGTACACCGAGCAATTCCTGGCACTGATTGGCCAGTTTATTCGCTCCACAATGGAGCAGTGCACAAGCCA GAAGATGCCTGAAATGCCTGCAGATGCTGTGGGCGCCCTTCTCTTCCTGGAGGATTATGTTCGGTATACA AAACTCCCCCGAAGGGTGGCTGAAGCCCATGTGCCTAACTTCATTTTTGATGAATTCCGAACAGTCCTGT GACTTCTTTCTCCTGCTCCAGTGACAGGATGTTCTATGACTAGAATGAACTTGGACGTGAGAAAGATACT TGACTCCTCATGCAACGTGCATTTTCCTCCTCTGTGGGAACCAGCCATTCTAAGGAAAGTGGCTCTCAAC TGTCTAAATCATGGCATCGTAAGGAATTTTTATCATGTAGATGCAGAACTGTTTTGTACTTGCCTCTTTT AAATGCTCAGTATAACTGATGGATGAAGCCATTCCATCTTATGTTCTAAGGATCTGTTTGTGCAACTCCA GACCTTCAGTAAAACAGCATTGCTAGTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30532658 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30532658 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17624 same embryo
	EMAGE:17625 same embryo
	EMAGE:17626 same embryo
	EMAGE:17622 same embryo
	EurExpress:euxassay_005959 same experiment
	MGI:4824455 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source