EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19516

Aurka aurora kinase A ( MGI:894678)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004878. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004878_01	euxassay_004878_02	euxassay_004878_03	euxassay_004878_04

euxassay_004878_05	euxassay_004878_06	euxassay_004878_07	euxassay_004878_08	euxassay_004878_09

euxassay_004878_10	euxassay_004878_11	euxassay_004878_12	euxassay_004878_13	euxassay_004878_14

euxassay_004878_15	euxassay_004878_16	euxassay_004878_17	euxassay_004878_18	euxassay_004878_19

euxassay_004878_20	euxassay_004878_21	euxassay_004878_22	euxassay_004878_23	euxassay_004878_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19516_wholemount_strong.wlz

19516_wholemount_moderate.wlz

19516_wholemount_weak.wlz

19516_wholemount_possible.wlz

19516_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19516_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 moderate expression: see section 11 12 13

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 16 17 18

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 moderate expression: see section 02 23

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 moderate expression: see section 08

orbito-sphenoid

strong

regional

strong expression: see section 05 06 21 22 23 moderate expression: see section 04 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6900
Entity Detected:	Aurka, aurora kinase A ( MGI:894678)
Sequence:	sense strand is shown >T6900 CTTGGAGGCTTCGGTCGGGAGCAGGTTCCTGCCTGTGAGTTGGAAAGGGACATGGCTGTTGAGGGCGAGC CTGGATGCTGCAAACGGATAGGGAAGGCTGTGTGGCGTCGGGGTGACATGGACAGATGTAAAGAAAACTG TGTCTCCAGGCCTGTTAAGACCACTGTTCCCTTCGGTCCGAAACGCGTCTTGGTGACTGAGCAGATTCCA TCTCAGAACCTAGGATCTGCTAGCAGTGGCCAGGCCCAGCGGGTCCTGTGTCCTTCTAACTCCCAGCGTG TCCCTTCACAAGCCCAGAAACTTGGAGCAGGTCAGAAGCCGGCACCAAAGCAGTTGCCAGCTGCCAGTGT TCCTCGACCTGTGTCCCGGCTCAATAACCCCCAGAAGAATGAGCAGCCTGCAGCCTCCGGAAATGATTCT GAAAAGGAGCAGGCATCCTTGCAGAAGACCGAAGACACAAAAAAAAGGCAGTGGACTTTGGAAGATTTTG ACATTGGCCGCCCACTAGGAAAAGGGAAGTTTGGAAATGTCTACTTGGCGCGGGAGAGACAAAGCAAGTT CATCCTGGCTCTGAAGGTGCTGTTTAAAACACAGCTGGAGAAGGCGAACGTGGAGCACCAGCTTCGGAGA GAGGTGGAGATCCAGTCGCACCTGCGGCACCCCAACATCCTCAGGCTGTATGGCTATTTCCATGACGCCA CCCGAGTTTATCTGATTCTAGAATATGCGCCCCTTGGAACAGTCTATAGAGAGCTCCAAAAACTCTCCAA GTTTGACGAGCAGAGAACAGCTACTTACATCACTGAGTTGGCAAACGCTCTGTCTTACTGTCATTCAAAG AGAGTGATCCACAGAGACATTAAGCCAGAGAACTTACTGCTTGGCTCAAACGGAGAGTTGAAGATTGCAG ACTTCGGGTGGTCGGTGCATGCTCCATCTTCCAGGAGAACCACAATGTGTGGCACCCTGGACTACCTGCC CCCAGAGATGATTGAAGGCCGGATGCATGACGAGAAGGTGGACCTCTGGAGCCTGGGCGTTCTCTGCTAT GAGTTCCTAGTGGGGATGCCTCCTTTCGAGGCACATACGTACCAGGAGACTTACAGAAGGATATCTCGGG TTGAATTCACTTTCCCTGACTTTGTGACAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTAAAACACAA CGCAAGCCAAAGGCTAACACTAGCGGAAGTCCTTGAGCACCCTTGGATCAAAGCTAATTCTTCCAAACCT CCAACTGGCCACACTAGCAAAGAGCCAACCAGCAAATCATCTTAGGGAATCCTGAACCACGGCCACCGTG CAACTGTGAGAAACACCCTATTGAAGCCACCCTCCTGGACCCTCATGGCTGCCCTCAGGGTGCCTTGCTG AGCAACTAGTCCTTGGCCATGCATCCAGGAAACCCCAAGAACCATGCTGCTTTTCCTGATTTAGTAATCT GGAGGGAAGGTTCCACTGCAGCTGTCCCCTACCCTCACCCTGGGCCTGTGCCCCAAGGTGTCCTTGTGGG GGGGGTCTCACTGTGGGAAACTTACCAGAGATCACCTGTGTCGTAGCCTTCAAGTGCCAGAGTGTGTGTA TAACTTATTGGGTCACCAGGCCTAGGGAAACTGTTGGAATGGGTATGTAATCTTTTAAATGTCAAAATAA AGATTTGCATAGACTTTGACTTTTAAGTGACACCCATTCAGGGTCTTGTTTCCAGTCGTCTGAAGCCCGT GCGGGATTTGGATCCTCAGCCACTGCTACGGAGCCTGTGCCTTGTCTTGAGACCTGACTCGGGGGTTTCT TTTTCTGCATGTTAAAGGCTATTTTTTATGTTGGAGTTCTGTCATGTATGCAAACTTGTTAAATATATAT GTATACCATCTAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3968835 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3968835 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19512 same embryo
	EMAGE:19515 same embryo
	EMAGE:19513 same embryo
	EMAGE:19514 same embryo
	EMAGE:19511 same embryo
	EurExpress:euxassay_004878 same experiment
	MGI:4823358 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source