EMAGE mouse gene expression spatial database

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EMAGE:19542

Diablo diablo homolog (Drosophila) ( MGI:1913843)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004954. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004954_01	euxassay_004954_02	euxassay_004954_03	euxassay_004954_04

euxassay_004954_05	euxassay_004954_06	euxassay_004954_07	euxassay_004954_08	euxassay_004954_09

euxassay_004954_10	euxassay_004954_11	euxassay_004954_12	euxassay_004954_13	euxassay_004954_14

euxassay_004954_15	euxassay_004954_16	euxassay_004954_17	euxassay_004954_18	euxassay_004954_19

euxassay_004954_20	euxassay_004954_21	euxassay_004954_22	euxassay_004954_23	euxassay_004954_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19542_wholemount_strong.wlz

19542_wholemount_moderate.wlz

19542_wholemount_weak.wlz

19542_wholemount_possible.wlz

19542_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19542_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

strong

regional

strong expression: see section 06 07 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 07 17

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

trigeminal v nerve

strong

regional

strong expression: see section 10 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 06 08 11 12 13

cervical ganglion

strong

regional

strong expression: see section 06 08 15 16

thoracic ganglion

strong

regional

strong expression: see section 08 09 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8014
Entity Detected:	Diablo, diablo homolog (Drosophila) ( MGI:1913843)
Sequence:	sense strand is shown >T8014 GACAAGAGGTAATCCGTCCGGCGTCGGTCCGCCGCGGGATGGCGGCTCTGAGAAGTTGGGTGACTCGGAG CGTCTGCTCTCTGTTCAGGTACAGACAGCGTTTTCCTGTCTTGGCTAACTCTAAGAAACGCTGTTTCTCA GAATTGATAAAACCATGGCACAAAACTGTGTTGACTGGATTTGGCATGACACTGTGTGCGGTTCCTATTG CTCAGAAATCGGAGCCTCAGTCTCTCAGTAACGAAGCATTGACGAGGAGGGCTGTGTCTTTGGTAACAGA TAGCACCTCTACCTTTCTGTCTCAAACCACCTACGCGCTGATTGAAGCAATCACCGAGTATACTAAGGCT GTTTACACGTTAGTGTCTCTGTACCGACAATATACAAGTTTACTTGGGAAGATGAATTCCCAGGAGGAAG ATGAGGTGTGGCAGGTAATTATAGGAGCCAGAGTTGAGATGACTTCAAAACAGCAGGAATATTTGAAGTT GGAGACCACTTGGATGACAGCGGTTGGCCTTTCAGAGATGGCTGCTGAGGCTGCCTATCAAACTGGAGCA GATCAGGCCTCCATAACTGCCAGGAATCACATCCAGTTGGTGAAGTCACAGGTACAGGAGGTGCGCCAGC TCTCCCAGAAGGCAGAAACCAAGTTGGCTGAAGCACAGACAAAAGAGCTGCACCAGAAAGCACAGGAAGT GAGTGATGAGGGGGCTGACCAGGAAGAGGAGGCCTACCTGCGTGAAGATTGAGGGCCTGAGCCCAGTGCC CTGTCCATCCACTCTGTGGGGAAAGCGGTGGGTGGTATCCACCTAATGTTGTCCCAGCTCTGCAGAGCTA ACAGTCCTGCCATTGACAGTCAGACCAGAGCCTTCTGTGAGCTGTGCCTGGCCCCTTGTCTGCTCACACC CTCTGTGCCTGGTTCTTAGCTCTGGGCGTGGCTCAGCACTGTCCCTCAGCACTGCTCCTCTCTTGCTGAT CTTTTAGCTCACACCCAAAGCATTTCACCAACCTGGGCCAACAGAGGAGGGGCCTTTCCTGCCACACCCT TAAGTTCAGTAGCTGTTTAACTACAGTTTTTAATGTTACTCGGATGTTCAAGTGTGATTTAGTGCTTGCT TCTCCACAGGGAAGTGGGGGGCTTGTCTGGTTTGTAACATTTCTGTGTTTATAATGTCCTTTCTCCCTGT GAGCACAGCTCAGCTATGGAGTTACTCAGGGCGTGAACAGCTCACCTGGTGCTCCCCACAGGCCCTTCTC TAACACGTACTTCTTGTTTGTTCCTTCTTTGAATTTCTCTGCTGTATCCAAAGGAAGAGAAGTTTAGTGT TTGCATTAAAAAAGAGAGATTGAGAGTGAGAGTGAGTCTTCAGGCTGTCCTGCAGCGTCTTCCTTCGCTC CCTAGGCAGTGGGAGCTGGGGGCCCTGGGGTCCTAAGGCTCCCTGGCAGCTGACACTCCCATTCAGCACA TGCTAGAGAAGTTTGCCAGGTTTCAGCACTTTTAATTAAATAAGGTTGTTAGCATAAAAATAAAGATCAT TTCCTGCTGACCACCAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30013833 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30013833 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19539 same embryo
	EMAGE:19538 same embryo
	EMAGE:19543 same embryo
	EMAGE:19541 same embryo
	EMAGE:19540 same embryo
	EurExpress:euxassay_004954 same experiment
	MGI:4824304 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source