EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31239

Anxa8 ( MGI:1201374)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002433_01	euxassay_002433_02	euxassay_002433_03	euxassay_002433_04	euxassay_002433_05

euxassay_002433_06	euxassay_002433_07	euxassay_002433_08	euxassay_002433_09	euxassay_002433_10

euxassay_002433_11	euxassay_002433_12	euxassay_002433_13	euxassay_002433_14	euxassay_002433_15

euxassay_002433_16	euxassay_002433_17	euxassay_002433_18	euxassay_002433_19	euxassay_002433_20

euxassay_002433_21	euxassay_002433_22	euxassay_002433_23	euxassay_002433_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

oral epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06

rectum

moderate

regional

moderate expression: see section 09 10 11

urethra of male

strong

regional

strong expression: see section 09 moderate expression: see section 10 11

axial skeleton tail region

strong

regional

strong expression: see section 09

bladder

moderate

regional

moderate expression: see section 10 11 12

esophagus

strong

regional

strong expression: see section 13 14

trachea

strong

regional

strong expression: see section 13 14 15 16

thymus primordium

weak

regional

weak expression: see section 12 13 14 15 16

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 10 11 14

vibrissa

moderate

regional

moderate expression: see section 03 04 20 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 15 16 17

nucleus pulposus

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

naris

strong

regional

strong expression: see section 10 11 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5080
Entity Detected:	Anxa8, ( MGI:1201374)
Sequence:	sense strand is shown >T5080 CCACGCGTCCGCGGACGCGTGGGTGTGTCCAGTCACTGAGCAGAGGCCAACTAGTGTTTCTTCATCTCCC TGGGAAAAGCAGCAGACAGGATGGCCTGGTGGAAAGCCTGGGTTGAACAGGAGGGCGTCAGCGTGAAGGG TAGTTCTCATTTCAACCCAGACCCTGATGCAGAGACCCTCTACAAAGCCATGAAGGGGATTGGAACCAAT GAGCAGGCCATCATAGACGTGCTCACCAAGAGGAGCAATGTGCAGAGGCAGCAGATTGCCAAGTCCTTCA AGGCTCAATTTGGAAAGGACCTCACTGAGACCTTAAAGTCAGAGCTGAGTGGCAAATTTGAGAGACTCAT CGTGGCCCTCATGTACCCGCCATACAGCTACGAGGCCAAGGAGCTGCACGATGCCATGAAGGGCTTAGGA ACCAAAGAAGGAGTCATCATTGAGATCCTAGCTTCTCGGACCAAGAACCAGCTGAGAGAGATAATGAAGG CATATGAGGAAGACTATGGATCTACCCTGGAAGAAGACATCCAAGGAGACACGAGTGGCTATCTGGAGCG GATTCTGGTGTGTCTCCTGCAGGGCAGCAGAGATGATGTGAGCGGCTTTGTGGATCCAGGACTGGTCCTC CAAGATGCACAGGCCCTGCATGAAGCGGGTGAGAAGATTATGGGAACCGATGAGATGAAGTTCATCACTA TCCTGTGCACACGCAGTGCCACTCACCTGATGAGAGTGTTCGAGGAATATGAAAAAATTGCCAACAAGTG CATTGAGGATAGCATCAAGAGTGAGACCCATGGTTCGCTGGAGGAGGCCATGCTCACTGTGGTGAAATGC ACCCGGAACGTCCACAGCTACTTTGCAGAGAGACTCTACTACGCCATGAAGGGAGCAGGGACACGTGATG GAACGCTGATAAGGAACATTGTTTCCAGGAGTGAGATTGACTTAAATCTTATCAAGGGTCAGTTTAAGAA GATGTACGGCAAGACTCTCAGCAGCATGATCATGGCCGACACCAGTGGTTACTACAAGACTGCCCTGCTG AACCTTGTGGGCACTGACCTCTGAAAATGTCAAAAAATAGAACAAGGGCAAGAAGTGTGAGCCCAGCCCC TGCAGCTTCAGCCCCGACCATGGGTGGGGGTAGGGGACACCTCATCTGGCTTTTAGTCTTCTGTTGCCCA GTTTCTGATGCTTCTCATCCCATGCTTCTGACCCGGATGATGAGACCAGCCTGGGTTTCCCTTCCACCTC TCCCGTCAGTCATTTCTAGGTATGTGTATACCGCCCACCCTAGCATTACCTCTGAGCAAACGGAAACATC AACATGAAGAAAGAAGCTTGCAGTGTCCTGTGGGTCATTCCTTCCCCTTCACACAAAATCTCCTGGGCAC AGGGCACACAGATGCCTTGGCTCTGACCCATCTACTGAGTCAGGGTCTTCAGAGGAAGAACCAAGGAACA TGGATTTCTAGCAGTTGTCTTAGCTGCTAAGCATCATTATCAGGCAGCCAGTGAGCATGTGGGAGAGCAC CTCTATGAGAGGACACGGCCGTGGATCTGAGAAAGGGTCACATAGCTCTGCCAATCGGGACACTCACTTA ACGGAAATTGTTAGGCTCTAGGTGGAGGAAAATTCTTACACCATCCTAATAGCACGCATTAGATGGGATT ACAAATCCCCGTCCTGACCCAAGTACGCTGTGGTCTGAGAAACTCGGGCTTGTCCAGTCATTCCCTCCAT GCTGAAGCTTGCAGACTAGGGAGCCGCCCAGCCCTGAGCATGAATCCCATCCTCTGATCGTGGTCTCCGA GCCTGAAGAACATGACAGAACTCTTCTCAATATTCGTTGGGCTTTCAGAATCATAAACATTTGTACTTTC AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5322310 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5322310 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002433 same experiment
	EMAGE:31978 same embryo
	EMAGE:30965 same embryo
	EMAGE:31235 same embryo
	EMAGE:32057 same embryo
	EMAGE:29810 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source