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EMAGE:31961

BC022765 ( MGI:2387599)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31961 EMAGE:31961 EMAGE:31961 EMAGE:31961 EMAGE:31961
euxassay_005607_01 euxassay_005607_02 euxassay_005607_03 euxassay_005607_04 euxassay_005607_05
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euxassay_005607_06 euxassay_005607_07 euxassay_005607_08 euxassay_005607_09 euxassay_005607_10
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euxassay_005607_11 euxassay_005607_12 euxassay_005607_13 euxassay_005607_14 euxassay_005607_15
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euxassay_005607_21 euxassay_005607_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 06 07 08 15 16 17
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09
metanephros
moderate moderate
regionalmoderate expression: see section 06 08 09 14 16 not examined expression: see section 06
rectum
moderate moderate
regionalmoderate expression: see section 11
midgut
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17
urinary system
moderate moderate
regionalmoderate expression: see section 11
bladder
moderate moderate
regionalmoderate expression: see section 10 11
pancreas
moderate moderate
regionalmoderate expression: see section 07 08 09
adenohypophysis
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13
trachea
moderate moderate
regionalmoderate expression: see section 12
kidney pelvis
moderate moderate
regionalmoderate expression: see section 07 15
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 12 13 14 15
pharynx
moderate moderate
regionalmoderate expression: see section 11 12
lung
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4442
Entity Detected:BC022765, ( MGI:2387599)
Sequence:sense strand is shown

>T4442
TGCTTCTGCACCTGTCTCTTACACCTGCCCTGCAGCTGCTCCTACCCACCTCTCCAGGCTGAGTCTGGCC
AGCGCGACACCCTGGAGACACCCTCTCCTCCTCCAGTCTCACCTGCCTGGGCAGCTCCTACTGTCAACTC
CTCTAGGGAAAGTCGCGCCTCCAGCCCAGAGCAGTCCGGATTACAGGCCAGTTCCTTGTGACCTGTGGCT
CCTTCTACTTCAAGGAGAGCAGAAAGCTCTGCCTGGGTTTAGGGACCGCCATGACTGAAGTTGGTTGGTG
GAAGCTGACCTTCCTCCGGAAAAAAAAATCCACTCCCAAAGTGCTCTACGAAATCCCGGACACCTACGCC
CAAACAGAGGGAGGTGCAGAGCCCCCAGGACCTGACGCAGGAGACCCTCACAGCGACTTTAACAGCCGTC
TGGAAAAGATTGTGGATAAGAACACTAAAGGCAAGCATGTCAAGGTCTCCAACTCAGGCCGCTTCAAAGA
GAAGAAAAAAGTTCGGGCCATGTTGGCAGAGAACCCCAACCTCTTTGATGACAGAGAGAACAAAGGACAA
TGAAAGCGGCCGAAGAGAACCATAGCGCTGCCCTGCTCCCTGCCACTGGTGGGGTCCGAGGCAGGAGCTT
GCCACGCTGGCCTCTTTGGTCACAGCCACTTGGCGGTTGATCCCTGCTGGCAGGAACACCCGGTTCTTAG
GTTTGTATTTATGTGCCCCAGCTTTACGAAGAGCCTCCTCCTCCTATGATCACATACTCTGGTCCAGCGT
GAAAACTGTGACCTAGAAGTGCTGCTGTCCTTGACACAGTGGCTTGCCTCACAGGGAGGTGGCACAGCAG
AGAATAGACAGAGAGCAGGACGGACTCCACCCTCTCCCCAGGCAACAGGGAGAAGGGTGAATTTGATTCG
CTGCATCCTCTACTTTCTCTACCGTGTGACTGTTGCCTGGGCCAATCCAGGGAGAGCAATGTTTCCGGAA
GTCACGTGACCGTGGTTTCCTTGGTGCCAGAGGCGGAGTTCTTAAGCTTGAGATCGCCTCTTAATTCAAG
GAGTAAACTTCCTGGAACTTTGCTCCCAGGAGTGGAGGGGCAAGGGACACGCGTACTCCCCGGGGTGCTA
CTGAAGGCTAGCCCAGAAATACTCCTGACCTTGACCACCACTTGTGTGGGTGCTGAATTACATAACCCCA
AAGAGAGGCCTCCCTGACCGGCCCAAGGGTCCAAACTGGAGGAGTGGATGCGGGTTTCTCCTGACTCCTT
CCCTGATAGTATTTAAGACAGATGAGCACCAGGAGTGAATCCTGGAGCCTTGGATTTTGTTGGAAAAGTA
GGTTTTACTTAGGAATAAACAAGGCTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4925830 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4925830 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005607 same experiment
 EMAGE:31351 same embryo
 EMAGE:31906 same embryo
 EMAGE:29254 same embryo
 EMAGE:30925 same embryo
 EMAGE:30907 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS