EMAGE mouse gene expression spatial database

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EMAGE:7109

Jmjd5 jumonji domain containing 5 ( MGI:1924285)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002581. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002581_01	euxassay_002581_02	euxassay_002581_03	euxassay_002581_04

euxassay_002581_05	euxassay_002581_06	euxassay_002581_07	euxassay_002581_08	euxassay_002581_09

euxassay_002581_10	euxassay_002581_11	euxassay_002581_12	euxassay_002581_13	euxassay_002581_14

euxassay_002581_15	euxassay_002581_16	euxassay_002581_17	euxassay_002581_18	euxassay_002581_19

euxassay_002581_20	euxassay_002581_21	euxassay_002581_22	euxassay_002581_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7109_wholemount_strong.wlz

7109_wholemount_moderate.wlz

7109_wholemount_weak.wlz

7109_wholemount_possible.wlz

7109_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7109_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5212
Entity Detected:	Jmjd5, jumonji domain containing 5 ( MGI:1924285)
Sequence:	sense strand is shown >T5212 CTCGGTGTAGTCGGTGATAAAGCCAGCCGAAGAATCTTCGCCCGGACGGAACCAGTCGTCGCCAGCAACG AGAGACACAAACAAGTCCAAGCGCCAGAGAGGTGCTGATCATGTCAGAGGACACCACAGAGCCCCTGGTG GGGTCCAGCACCCTCTGGAAGGAGCTCAGGACGCTCCTGCCTGACAAAGAGGAGGAGCTGAAGCTGGACC TCGGTGAGAAGGTGGACAGGAGCGTGGCTGCGCTTTTGCGGCAGGCTGTGGGTCTATTCTACGCGGGCCA TTGGCAGGGGTGCCTGCAGGCCAGTGAGGCCGTCCTAGACTACTCCTGGGAAAAGCTCAACACTGGTCCC TGGAGAGATGTAGACAAGGAGTGGCGCCGGGTATATTCCTTCGGCTGCCTCCTGAAGGCCCTGTGCCTGT GCCAGGCACCACAGAAGGCCACTACTGTGGTGGAAGCACTGCGGGTGTGTGACATGGGCCTGTTGATGGG GGCAGCCATCCTCGAGGACATTCTTCTCAAAGTTGTCGCAGTCCTCCAGACACACCAGCTCCCAGGAAAG CAGCCTGCCCGCGGCCCCCACCAGGATCAGCCTGCCACCAAGAAGGCAAAGTGTGACGCCAGCCCAGCTC CTGATGTCATGTTAGAGCGGATGGTACCCAGGCTCCGCTGCCCACCTCTGCAGTACTTCAAGCAGCATTT TCTAGTTCCTGGGAGGCCTGTGATCTTGGAAGGTGTGGCCGACCACTGGCCGTGCATGAAGAAGTGGAGT CTGCAGTACATCCAGGAGATTGCTGGCTGCCGCACCGTCCCCGTGGAAGTGGGCTCAAGGTACACAGATG AAGACTGGTCCCAGACTCTCATGACAGTCGATGAGTTCATCCAGAAGTTCATCCTGAGCGAGGCAAAGGA TGTCGGGTACCTTGCTCAGCACCAGCTCTTCGACCAGATCCCAGAGTTGAAGCGGGACATCAGCATCCCT GATTACTGTTGCCTGGGCAATGGAGAAGAGGAAGAGATCACTATCAATGCCTGGTTTGGTCCACAAGGCA CCATCTCCCCACTGCATCAGGACCCCCAGCAGAACTTCCTGGTCCAGGTGCTAGGAAGGAAGTACATCCG ATTGTATTCGCCGCAAGAGTCTGAGGCAGTGTACCCTCACGAGACGCACATTCTTCATAACACCAGCCAG GTTGATGTGGAAAACCCCGACCTTGAGAAGTTTCCCAAGTTCACTGAGGCCCCATTCCTGTCCTGCATTC TGTCCCCAGGAGACACCCTCTTTATACCTGCTAAGTACTGGCATTATGTGCGCTCCCTGGACCTGAGCTT CTCTGTCAGCTTCTGGTGGTCATAGCTAGGACCAGACTGGAAAGGACCTCTGGGCAGACACCTTACCCAA AGGCAGTAAACCAAGTGTCAGTGTGGGCCCTGCATGCCGCTAGACTCCAGCAGCCCTGCCCCCAGCCAAT GTCTGTTGTGTGACAGAGCTGCTATGTACCCACGTGTGGCCCACCTGCTGTGTGTGCCCGCGTGTGTAGG GCAGAGCTGCTACATGTACCTAAGCTATCCAGATTATGGGTGCTTTTTTTCCTTTGTAACTGCTGGGCTG AGTATCCCTTGCTGCTAGGTGCCCAAGGGCAGCTGGCAAGCTGCTCAGAGACAGAACACAACCTGTAATA GGGGTTTTCTTGGGTGGAATATAGCCGGGTCTGGGACAGCTGCTGTGGTCCCAGTCTTCTGTGTACCCAG GCCTCACTGCCAGCTTACTACTAGGAGTTGGAAACAGAGGTCCTTGGCTCCATACTTCCCCTCGGGGCAC TTATGCACCAGGCTGGAAGAGGAGGGCAGCGGAACTACTTTGTTGTGAATGTGGGGAATACAGAGAAGAG CGAGGTCTTCCCAGAATATATAGGAGCGGCCTTTTACAAGGAAGCCCCTGCCCCCAAGCCTCCTTGATGA AGAGATGGTTCTTACGTTCAAACTGTTTTAGTTGATGGCGGATGTGCATGCACACACCTTACTCAGGGTG AATCAAGGAGTAAATACCTCTTGTGGGAAGGACTGCCCAGGAAGGGAAGCTCATTGGCTGAACACTCGGG GCCCACTTAGATACCACATTACCATGGAGAACTCCAGGTTTTTATGTTACAAGACTGACTGTCACCGTCC TCTCAGTGGGGTGTCATGTTCCAGATCAGGTAGTTTGGCAGGGAGCTGGCTCTACTCCAGCTGTTCTCAA TTCTGAGATTTCCCCAGTCTGAGCCTGCTCAACCTTACCAGTTCTTCTGTCTGGTGGCCCGGTCCACTTG CCCCATATGTAACTTAAATATTTTCAATAACAATAAAATATATTTTATTAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5355270 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5355270 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7114 same embryo
	EMAGE:7111 same embryo
	EMAGE:7110 same embryo
	EMAGE:7113 same embryo
	EMAGE:7112 same embryo
	EurExpress:euxassay_002581 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source