EMAGE mouse gene expression spatial database

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EMAGE:7236

Prepl prolyl endopeptidase-like ( MGI:2441932)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004469. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004469_01	euxassay_004469_02	euxassay_004469_03	euxassay_004469_04

euxassay_004469_05	euxassay_004469_06	euxassay_004469_07	euxassay_004469_08	euxassay_004469_09

euxassay_004469_10	euxassay_004469_11	euxassay_004469_12	euxassay_004469_13	euxassay_004469_14

euxassay_004469_15	euxassay_004469_16	euxassay_004469_17	euxassay_004469_18	euxassay_004469_19

euxassay_004469_20	euxassay_004469_21	euxassay_004469_22	euxassay_004469_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7236_wholemount_strong.wlz

7236_wholemount_moderate.wlz

7236_wholemount_weak.wlz

7236_wholemount_possible.wlz

7236_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7236_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 14 15 16 17 18 19 20 21 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 02 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04 05 06 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 16 17 18 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 15 16 17

trigeminal v nerve

strong

regional

strong expression: see section 09 16

spinal cord

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 09 14 15

cervical ganglion

moderate

regional

moderate expression: see section 06 07 08 14 15

thoracic ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 13 14 15

retina

strong

regional

strong expression: see section 01 02 03 04 21 22 23

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16

bladder

moderate

regional

moderate expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6440
Entity Detected:	Prepl, prolyl endopeptidase-like ( MGI:2441932)
Sequence:	sense strand is shown >T6440 GCTGGAGGCTGGCTTCGGCTGGACAAGGCTGAGGTGACTGCAGCTCCTTCTGGAGAGCTTCGGTTTCTCG TATTTGTCTTCTCTACCTTCGCTCGTCCAGCTGTCTTTCGATTTGCGATCTTCTGCCTATTAAGCCAGAA AGTGAAAAGCCCATTTCTGAAAACATGGATGCATTTGAAAAAGTGAGAACAAGACTGGAAACACAGCCAC AGGAAGAGTACGAAGTCGTCAATGCAGAGATTAAACATGGCGGCTTTGTTTATTATCAAGAAGGTTGCTG CTTGGTTCGTTCCAAAGACGAAGAAGCTGACAGTGATAATTATGAAGTTTTATTCAACCTGGAAGAACTT AAATTGGATCAGCCTTTCATTGATTGCATCAGAGTTGCTCCTGATGAGAAATACGTGGCTGCCAAGATAA GGACGGAGGATTCTGAAACCTCCACCTTAGTAGTTGTGAAGCTCAGTGACCAGCCTGTAATGGAAGCGTC GTTCCCAAACGTGTCCAGTTTCGAATGGGTGAAGGATGAAGAAGATGAAGATGTCTTATTCTACACCTTC CAAAGGAACCTTCGCTGCCACGATGTGTATCGAGCCACATTTGGTGACAACAAGCGCAATGAACGGTTCT ACACAGAGAAAGACCCAAGCTATTTTGTCTTCCTCTACCTTACAAAAGACAGTCGTTTCCTCACACTGAA TATTATGAACAAGACGACTTCCGAGGTGTGGTTGATAGATGGCCTGAGCCCGTGGGACCCACCAGTCCTT ATTCAGAAGAGGATACACGGAATGCTTTACTATGTTGAGCATCGAGATGATGAGTTATACATCCTGACGA ATGTTGGAGAACCTACAGAATTCAAGCTGATGCGGACGGCAGCTGATGCCCCTGCTATTATGAATTGGGA TTTATTTTTCACAATGAAGAGAAATACCAAGGTTGTAGACTTGGACATGTTTAAGGACCACTGTGTTCTG TTCCTGAAACACAGCAATCTCCTCTATGTTAATGTGATTGGTCTGGCCGACGACTCAGTGCGCTCCCTAA AGCTCCCGCCTTGGGCCTGTGGATTCATAATGGATACAAATTCTGACCCAAAGAACTGCCCCTTTCAGCT TTGCTCTCCAATAAGACCCCCAAAATATTACACATACAAATTTGCAGAAGGCAAACTGTTTGAGGAAACT GGTCACGAAGATCCGATTACAAAGACCAGCCGTGTTTTACGCATAGAAGCCAAAAGCAAGGATGGAAAGT TAGTGCCCATGACAGTTTTTCACAAAACCGATTCTGAGGACCTGCAGAGGAAGCCTCTCTTGGTGCACGT CTATGGAGCGTACGGAATGGACCTGAAAATGAATTTCAGGCCTGAAAAGCGGGTCTTGGTGGATGATGGA TGGATCTTAGCTTATTGCCATGTTAGGGGTGGTGGCGAGCTAGGTCTCCAGTGGCATGCCGATGGCCGAC TAACTAAAAAGCTCAATGGCCTTGCTGACTTAGTGGCTTGCATTAAGACGCTTCACAGCCAGGGCTTTTC TCAGCCGAGCCTAACAACGCTGAGCGCTTTCAGTGCTGGAGGTGTGCTCGTGGGAGCACTGTGTAATTCT AAGCCAGAGCTCCTGAGAGCCGTGACCCTGGAGGCACCTTTCTTGGATGTCCTCAATACCATGTTGGATA CCACCCTTCCTCTGACACTGGAAGAGTTGGAAGAGTGGGGGAACCCGTCATCTGATGAGAAACACAAGAA CTACATAAAGCGCTACTGTCCCTGCCAGAACATTAAGCCTCAGCATTATCCTTCAGTTCACATCACAGCC TATGAAAACGATGAGCGAGTGCCGCTGAAAGGAATCGTGAACTACACAGAGAAGCTTAAGGAGGCCGTGG CTGAGCACACCAAGGGAGCGGGTGAAGGCTATCAGCCCCCCAACATTATCCTAGATATTCAGCCTGGGGG GAACCACGTGATTGAGGATTCTCACAAAAAGATCACAACCCAGATGAAATTCCTATATGAGGAACTTGGA CTTGACAGCACTGATGCTTTCGAGGCGCTGAAGAAATACCTAAAGTTCTGAAATGCTGCATTCAGCTGGG AACTGGAAACACATGGAAATAATAATTAGTTTTGTTTCCAGTTAGTTTAGCAAAAGTGAAGTTTTTTTTA AGTTATTATATAATTTTTTAAAAGTTGTCTCTTTGTGTAATTTTGCTTAATACTTGCTCATACTGTTCCC ACCAGCACACACCCCCTGGCCTAGGAAAGTCGTTTTCCATCATGCTCCTCAGTAGGATGTAGAGAAGGGA GCCAGAAGGACTGGGGCAGGAGAACAGGAGAACGCCTGGCCTCCCAATCAGTCTTTTAACAGAATTTTAC AATTCAAAATCCTTCCCCTTTGCTAGTGAATATTTGTAGGCGCTGTGGCTGTGAGTCACCTTGCAATTTC CACAACTCCACTTTAAAGAAAAGGATAGCATTCTTCATATTTAAATGCTTTAGTATTTTGGCATAATCTT TTAAACACTTTAAAAACAGTGCATTCGTGCTACAGAAATATCTAAGAATTGATGCCATTCATGAGTCTGC TCAGCCCTGTGCGTACGTGATGTTCTGGGTGGCCATCTCTTAGGGAGCTTCCTTCCTAACACGAGCTATA GAGGATGTCCAGTGCACTGGAGTAGCACGCCCGACTGGAAACAAAGCATCTGTCTCTGAAAGCGGCCTGC TGATGGAGGGGAGCCTTCGTGCTGTGCTCCAAGACCAGGCCCTCTCCCTTCTCCAGAGAAATGGCACGGG TGTCAACAGCACTGCCTTTGGAGGCTGAGTTGGTACTTAACCTTATTCTCAGGTCTGGAGGAAGATCTGA AATGATCCCCTGTAGATTTAGCACAGTTGATGATTCTCCAAAATTCAGAACCACGAGGAAGACGTTATCT ATGCCGTCCAGCTCTCTTGTGTACACCACAGAGTGACTGTCGTCTCTCAAGAGGCAAAACCAGCCCCGGC TGAGGACCAGCTCTGTGGCATGGAGTAGACTCAGATCCTGATACAGCCTCAGTGCTGAGCTCGGCTGGGT CTTTTGGACCTGTTTTTAAAAAGAATATTGACATAAATGTTTGATTTTAAGAATTATCCACACCCCCCCC AAAAAAATCCCTTGCTTCTGTTTGCCCTTTACACTGTCCAAGTAAGTAAATAACTGCCCAATTATAAAGC ATTTATAAATAGTTGGTCCTAAAGTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3585402 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585402 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7233 same embryo
	EMAGE:7234 same embryo
	EMAGE:7232 same embryo
	EMAGE:7231 same embryo
	EMAGE:7235 same embryo
	EurExpress:euxassay_004469 same experiment
	MGI:4827406 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source