EMAGE mouse gene expression spatial database

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EMAGE:7567

Ado 2-aminoethanethiol (cysteamine) dioxygenase ( MGI:2685083)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004673. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004673_01	euxassay_004673_02	euxassay_004673_03	euxassay_004673_04

euxassay_004673_05	euxassay_004673_06	euxassay_004673_07	euxassay_004673_08	euxassay_004673_09

euxassay_004673_10	euxassay_004673_11	euxassay_004673_12	euxassay_004673_13	euxassay_004673_14

euxassay_004673_15	euxassay_004673_16	euxassay_004673_17	euxassay_004673_18	euxassay_004673_19

euxassay_004673_20	euxassay_004673_21	euxassay_004673_22	euxassay_004673_23	euxassay_004673_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7567_wholemount_strong.wlz

7567_wholemount_moderate.wlz

7567_wholemount_weak.wlz

7567_wholemount_possible.wlz

7567_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7567_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8654
Entity Detected:	Ado, 2-aminoethanethiol (cysteamine) dioxygenase ( MGI:2685083)
Sequence:	sense strand is shown >T8654 CGGGCGACCGTGCAAGGCGGCCCCGCGCGCCCCGCTGGCCGGTTGTTGCGGGGAGGTCTCCCGCCTGGCG CGGGGGTCCGCGCGGGTAGCCGTGGCGGCCGCCGGAGACCGCAAGGGGCGGCGCAGAGGCGGAACGGGCC GGCCGCGAGCCGCGCAGGAAGCGCCAAGCATGCCCCGCGACAACATGGCCTCCCTGATCCAGCGCATCGC TCGCCAGGCGTGTCTCACCTTCCGCGGCAGCTCGACGGGCTCCGAAGGGCCGGCGCCGGGCTTCCCGGAG AACCTGAGCCTGCTCAAGAGCCTGCTGACCCAGGTGCGCGCCGAGGACCTCAACATCGCGCCGCGCAAGG CGCTGCCGCAGCCGCTGCCCCGCAACCTCCCGCCGGTCACCTACATGCACATCTACGAGACGGAGGGCTT CAGCCTGGGCGTGTTCCTGCTCAAGAGCGGCACGTGCATCCCGCTGCACGACCACCCGGGCATGCACGGT ATGCTCAAGGTGCTGTACGGCACGGTCCGCATCAGCTGCATGGACAAGCTGGACACGGGGGCCGGGCATC GGCGGCCGCCGCCAGAGCAGCAGTTCGAGCCCCCGCTGCAGCCCTTGGAGCGGGAGGCCGTGCGACCGGG CGTGCTGCGTTCCCGGGCCGAGTACACCGAGGCCAGTGGGCCCTGCGTGCTCACTCCACACCGGGACAAC CTGCACCAGATTGATGCCGTGGACGGGCCAGCTGCCTTCCTGGACATCCTGGCCCCACCCTACGACCCGG AGGACGGCCGGGACTGCCACTATTACCGTGTAGTGGAGCCCATCAGACCCAAGGAGGCTTCCGGCTCTGC CTGCGACCTTCCCCGAGAAGTGTGGCTCCTGGAGACACCACAGGCCGACGACTTCTGGTGCGAGGGAGAG CCCTATCCAGGCCCCAAGGTCCTACCTTGAAGCTCCTGCCGCCCGGGGAACTGTGGGCCAAAGATGTACC CTGCCCTGCTCAAGAAAGGCCTGGCTGCCCTCTATCTTCCATAAGGGCTCTTCTCTCCGCCCCCTCGCCT GGACGATGGATTTACTGGAGTGACCAGCGCCACATCTTGAGGAACCTTGGGGAACACGGCCCACTGGAGT ACAGCCACCACCGGGCACGGTTATGGGGGCGGGTGGGCGGAAGGCTAGGTTGATTCCTGGTATCCTCAGT GCCACCAAGGCTTTGATTTAAGGGAACCGGGCAGGGACCCTAAAGCGCTTCCATCCTAAAGCCATAATGA AACCATTTTTGTTTCTCATTCTCTACAAAATACACTAAGTAGTTTTGAATCCCTCTTCCCTTCGGTGACT CAGATTTGTTTTCTCTCCTGCCCACGATTGCTCCTTAATAGTTTAATTTATTACTGATTGTGCATGACCT TGAATTGTTCTTGTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTAGTTATTCGTTTGGTTAAAACACACAC ACAAACTACTCAGCATCTAAAGTGAGGAGTTCTGAAAAGACATCTTTCTAGCACTGGGGTTCACCCTCAT AACTGTGCGTGTTGAGGACGATGTCTTTCCTCCCCTGGCTTGGTAGCCACTTCTGGTGTACTCAGGTAAC CTTTAGTAAGAAGGTCACATACGCGAATTCTCTTGTCAGGCCCTTAAAGCTCGTCTTTCCCTTGGGTCTC AAGATCTGTCGTTTAAACAAATTTTTTTGTTGCTATTGGGGATTTGCCTTTAAAGCAAAGACGATTATTA TCCTCAGCTGACTTTTTCCTTGACGTGCTTCAAAGATGATACAATAATTATGTGTTTTCTAACCAGACGA TTCCGAGGAGCGGAATCTGCTATGCTTCCAAATAACTGAATGTAAAGGCCCTGGCCACCTGTTGAATTCC ATACTCGTATTTACTTCCAGCTTCTTCCTAGGAAAGAAGAGAAAGATACTCTTGACTGTTTGTTGTTGTT GTTGTTGTTGTTGTTGTTGGTTTTGTTCTGTTTTGGTTTTTTGTTTTTGTTTTTTTGCGTTCTCTGTGGA GGGAGCCTGCAGAGGAGCTGTGTGGTTCCAGCTGTGCTGCCGGGAGATAGCCTCCCAGCACTCCCTTAAA CAAGAGCCAGTGCTGCCTACTACTGCCTTCTCTTGCAGGAAGAGGTAGAGAGCATGTAGTACCCTGGGAG GGGCTCTGGAAGTAGGCTGCTGGCTAAGCAGGCAAGAAGCCCTGGGCTCAATCCTCAGTACCACATAAAC CAAGTGTAGCACACAGCATTTAGGAAATATAGGCAGGAGGATCAGAAGTCAAGGTCAGACTTGGCTACAT AGTGAATTTGAGCGGAACCGTGGATGCATAAGACACCATCATATAAATGTGTGTGTATACATAACATATG AATATGTATATATACACATATACATATGTATGTACACACACACATATGAAATGGATACATGGAATAGATG CTTGAGAATTTAGCCATTTAGGGCACCAAGATTGGAGACTAAACCCTATGCCAACACTGCAGCAAACCCT AGCGCTGCTGCAGTATTATGGCTGGCGGAGGCTCAGCCCCTTTACCATGTTTACAGACGTGTCTATCGAT TCTGACCATGGACCACCAGTTTCCCCTGTTTACTATGTGTAGGGTTCCTTTTTATCACAGGCCTTTTCAT AAAGACAAACTGTAGAAATCAGATGTGCCCAGCTGTCTTCTAAGCTCTCTTGATGGCTCATGTGGCAATA GAATATACTAATATCGGCAATAAATGGAGACTTACTGTCATGAGTGACAAATGTTTGTTGACCTGGCAGG GAAACAAACTGCTTTTGGCTCCATGTATCCAGGATACACATCCAGGCTGAAGGGGTGTCCTTCCCGGTGC AGTCATTAAAGTGGTACGAGACAGCAAATTATTGCTGGCTTGGCTTGTGGCTTTTTGTTTGTTTGTTTGT TTGTTTCATTTTAATAAAAATGAGAACAACAGGCCAGTGGAAACCTGCTTTAAACCTGACAAACCATGAG CAACACCCAAACACTTCCACCATGGCTGCTAGAGTTCAAGTTCTTTCTGATCATGGTCTTTAAAATGCCT TTGGAGCCTCTCGAAGCAGCTCTTTGAGCCACTCGTATGAACATCTAGATAGGAAGTGATGGTTTTAAAT TTGACTATTTTCATAGAATATTAATAATGAATATGGTTTTTCAGCCACATACAGATTTTGAATTGCAGCA AAGGTTGATTCCTTTACAAGGATTGTTGAGAGTCATGTGTACTTTACAGCATACTCTGGAGGTCCTTTCA TGGAGCCTGTAGAAGTCTAGCCTGGAATTGGGCTAGTAATAACAATTATTATGAAGGCCTTCATTGCAAA AGAAGGAGGAGAAATATTAAAGGTTTGTTATAAGCAGTTTGGAAGGAAAAAAACCCTAAGGTGCTTTTCA AAAGAGTAACGAAATTGTTCTGAGCTAAAACAGCAATACTGTGTTCCTCATTAGTTCAATAGGAACAGTG AAACTGTCATTTCACTAATAAACTGAGTAAACTTACGGTGAAAACAGTGTAATATGTTTTAATGAGCCAC TTGAGAAAGAATATGCTCGTTACTAAGATAAAATGTGCAGAAAAATCCTTGCACGTCTATGCATAAACTA TTAGCTATAACTTCTGCCGTTGTGTTTCCCTGTAGGTCTACAATATAGATTGTGGTCTAGAAATTAGGAT AAAAGTTACCTTCTCCTTTTCTTAGAAAAGACCAATGAGCACAATGGTGTTAAGTCACTTAACTGTTCTG ATGAATAATAAATGCAATAACTAGATCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5721417 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5721417 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7570 same embryo
	EMAGE:7571 same embryo
	EMAGE:7569 same embryo
	EMAGE:7568 same embryo
	EMAGE:7566 same embryo
	EurExpress:euxassay_004673 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source