EMAGE mouse gene expression spatial database

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EMAGE:7643

Sh3bgrl SH3-binding domain glutamic acid-rich protein like ( MGI:1930849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005316. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005316_01	euxassay_005316_02	euxassay_005316_03	euxassay_005316_04

euxassay_005316_05	euxassay_005316_06	euxassay_005316_07	euxassay_005316_08	euxassay_005316_09

euxassay_005316_10	euxassay_005316_11	euxassay_005316_12	euxassay_005316_13	euxassay_005316_14

euxassay_005316_15	euxassay_005316_16	euxassay_005316_17	euxassay_005316_18	euxassay_005316_19

euxassay_005316_20	euxassay_005316_21	euxassay_005316_22	euxassay_005316_23	euxassay_005316_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7643_wholemount_strong.wlz

7643_wholemount_moderate.wlz

7643_wholemount_weak.wlz

7643_wholemount_possible.wlz

7643_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7643_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8937
Entity Detected:	Sh3bgrl, SH3-binding domain glutamic acid-rich protein like ( MGI:1930849)
Sequence:	sense strand is shown >T8937 AGAGCTGGAAGCTCATTGAAAGCAGCCGCTGGTGGAGGCGGGGCTCTGGCCAGTTATCCTTCCACCTTCC CCCACCCTCCTCATCCAACCTCTGTTTGGCCCTTAGCTCGGTTCCAGCCGTTGCTGCAGCTGTTTCCCAG CCCTTTTCAAGACTCCAGCGACCGACCAGAAGCCCTTGTTTCCAGGATGGTGATCCGTGTATATATTGCA TCTTCTTCTGGCTCTACGGCGATTAAGAAGAAACAGCAAGATGTGCTTTGTTTCTTAGAAGCCAACAAAA TAGGATTTGAAGAAAAAGATATTGCAGCTAATGAAGAGAATCGGAAGTGGATGAGAGAAAATGTACCTGA AGACAGTCGACCATCCACAGGGTACCCCTTGCCACCTCAGATTTTCAATGAATGCCAGTACCGTGGGGAT TACGATGCCTTCTTTGAAGCCCGAGAAAATAATGCAGTTTATGCCTTTTTAGGCTTGACAGCACCACCTG GTTCAAAGGAAGCCGAAGCCCAGGCAAATCAGCAAGCATGAATGTTCTGAGTTCTTCCTTCAGCATCTTG AAAAATAATGAAATCTCCATTGCTTTTATAGAAATCCTTGCTTCAAAAGAAATAGGCTTACATGTTGAAA TAATAGATTAGAGATTAGTTGATATTCTCGCATGCAAACAAGCATAATGAAAATGTGAACATGGTGGTAA GAAATGAGAGATGAAGTTAAAACTTTAAGTAGATATCTTAGAGTAGCATTGTTATCTGCCAAGGCATTTC TGTTCTGTAAGGATTTTACAAAAAATCAATATGCTCCTTCTTTTAAATTTCTGTGTAGCTTAAGGTTCTC ATCATTTCAACTTTAAGGAACTGAAAGGAGCGAGAGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAGAGAGAGAGAGAAGAGAGAAGAGAGAGAGACTCTTATTCCCACAATGCCTCATTATCTTTAGTG AACATGAATATTTTCATGTGATGTTACTGTTGTTGAAATGTATTTACAGGTTATGTTCATATGTTAGTCA TTGTAATTTCCTATGCTGATTGTGACATTCTCCTTCTTTTATCAAAGAAACATGTATGCCCCGAACCACA AGTTTTCCAGGCTTCTAGATGTAACCTTTCATTGCACAGCCAGTGATCAAAATAGCTGGCTTCCCGTCAG ACACACGTTGAGTGCATTTAACTGATAAATTCCTTGCTTATACATGCAGTTATAGTCATGTTTCTAAGTT GCTTGCCTCAGTACAATGTGGGTATATAAGTCATGGAGAAAAGATTGCAGTTCACTTTAAATTGGCCCTT CCTTGCAAAGAACACAGACTTTTAATTAACTGTTATATAGTATCTTGCCCTTCATTGTTTAAGATATATT TTCTTTTGGTGTTTGCTGAACGAAGTAGTAGTGGCTCTTGTCTCCTGCTTTAACACTGTTGGTGATCGTG AATGATACTGTCCTTAATAGGTAAGCTTTTAGTTTCACTTTAGGTAGACTGTGTCAACACATCCGTCCAA GTAGAAAGAATCACTAAGCACACATTGATTTTTTGAGAAAGTACAAGTATCAAAGAAAAGCAACAACGTT TGAAGTAGTTTGAAGATAAGCAAGACCCAAAGCATTCAACCAGAAGATGTTGATATTGTACCTTTTTCCC ATACTTGATTTTTATAAAGAAACACACATCTTTTACTTCAAATTACTATTATCATCATACCTGCTCTTTC CTAGACTGTACTTGGCAAAAATTCAATATGTAATGGAGCCATAGCTTAGTTTACTGAAACTCAAACGTGA ATTGATGCTTTAACATAAATGGTTTTAAAATTTGCGCTACTCAACTCTAAATGTTATACAGTAATTTTGA TGAATAATTTTTGTTTATAGGTGTTTAAAAAGGAGAAAAACCAAAATGAACCAAACCAACCAAAACAACA ATAAAAAACAATTTGAAGGCAACTTTTAGTGACCTAAACTTTGAAGTATGAATTGGAGTCAAAACTATTC CATTTCTTCTTCCTTTTTTTTTTTTAATTGTTTTGGGGATCTCTATGGAAGACAATACTTTAAATTAATT TTGGAGTGCAAAATACTTTTAGCCAATGTTTTCCCTCATCATTGCTAAATGCTACAGGTAATACCTGCAG TATTATATGTGGACAATGTAACTTTCTCTGTTTTGCATTGGAGGATGTTTATGTCTGTGGGATAATACCC TGAAGTCTGTAGATTTAAAGTACAAGTTTGAATTGAGTCTTCTCCAATTGAAGCCTGTCATTTTGTCAAC TGAGAAACATTCCTGATAGATGAAAGGAAAGGAAATAAAAGTCTAAGAATTAAATGAGTTTTGAAGAAAC TTCCACAGTATATGCTTGGGAGTGAATTTCTTGTTGCTTTGCATAATATGTTTGGCATATTTGTGTTTCT AAATGAGACTTGGTAATTTGTAAATTTCACATTTAAGAGCTGATTTGCCCTTTAGAAATTTTGTTCTTTA GAAAGAGTTCCACTTTCAAAAACACTGTAAATTGTTAATTGTACTGCTAAATGTGGACACTTCATTGGGA CCCTAAGTATATGTGAATAGGGAATTTTGAAACAAAGATTACTTTTACTGGGAAAGACTGAGTACAGGGA GTTTTGGTGAAATCAGACTTCACTCCTTAAGTAAAAAGAACAACAAAATGTAAAACTCACATGAATTTTT GTTAAGCAGTTGTTGGTGTTTAAAATTACAAAGTACAACATTAATCCTTTTCTATGGAACACTGATTCTT TTCATCTGATGAATTTCAATAAAACTGATTAAGTGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6418767 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418767 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7648 same embryo
	EMAGE:7647 same embryo
	EMAGE:7645 same embryo
	EMAGE:7644 same embryo
	EMAGE:7646 same embryo
	EurExpress:euxassay_005316 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source