EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7648

Csf1r colony stimulating factor 1 receptor ( MGI:1339758)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005275. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005275_01	euxassay_005275_02	euxassay_005275_03	euxassay_005275_04

euxassay_005275_05	euxassay_005275_06	euxassay_005275_07	euxassay_005275_08	euxassay_005275_09

euxassay_005275_10	euxassay_005275_11	euxassay_005275_12	euxassay_005275_13	euxassay_005275_14

euxassay_005275_15	euxassay_005275_16	euxassay_005275_17	euxassay_005275_18	euxassay_005275_19

euxassay_005275_20	euxassay_005275_21	euxassay_005275_22	euxassay_005275_23	euxassay_005275_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7648_wholemount_strong.wlz

7648_wholemount_moderate.wlz

7648_wholemount_weak.wlz

7648_wholemount_possible.wlz

7648_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7648_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

single cell

strong expression: see section 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8934
Entity Detected:	Csf1r, colony stimulating factor 1 receptor ( MGI:1339758)
Sequence:	sense strand is shown >T8934 GAACTAGCAGCTGGGAGCCCCGTGCCCAGCCGACTCTCCAACCTGCATCGGCTCACGCTATCCCCTGGAG GCTATGGAGTTGGGGCCTCCTCTGGTCCTGCTGCTGGCCACAGTTTGGCATGGTCAGGGGGCCCCTGTCA TCGAGCCTAGTGGCCCAGAACTGGTTGTAGAGCCGGGTGAAACGGTGACCCTGCGATGTGTGAGCAATGG CAGTGTGGAATGGGATGGCCCCATCTCTCCCTACTGGACCTTGGACCCTGAATCTCCCGGAAGCACCCTG ACCACAAGAAACGCGACCTTCAAAAACACTGGGACCTACCGTTGTACCGAGCTTGAAGACCCCATGGCAG GCAGTACCACCATCCACTTGTATGTCAAAGATCCGGCCCACTCTTGGAATTTGCTGGCACAGGAGGTGAC AGTGGTTGAGGGCCAGGAAGCTGTGCTGCCCTGTCTGATCACTGACCCTGCACTGAAGGACAGTGTCTCA CTGATGCGTGAGGGGGGCAGGCAGGTCTTACGCAAAACGGTCTACTTCTTCTCGCCATGGCGAGGGTTCA TTATCCGCAAGGCTAAAGTCCTTGACAGCAATACCTACGTGTGCAAGACCATGGTGAATGGTAGGGAATC CACCTCCACTGGCATCTGGCTTAAGGTGAATCGAGTCCACCCAGAGCCCCCACAGATAAAATTGGAGCCT AGCAAGCTGGTGCGGATTCGAGGGGAGGCTGCGCAGATCGTGTGCTCGGCCACTAACGCCGAAGTGGGAT TCAACGTTATCCTCAAACGTGGAGACACCAAGCTGGAAATCCCCCTAAACAGTGACTTCCAAGATAACTA TTATAAAAAAGTCCGGGCTCTCAGTCTCAACGCTGTGGACTTCCAAGACGCTGGCATATATTCTTGTGTG GCCAGCAATGATGTTGGCACACGCACGGCCACCATGAACTTCCAGGTGGTGGAGAGTGCCTACTTAAACT TGACCTCTGAGCAGAGCCTCTTGCAGGAGGTGTCTGTGGGTGACAGCCTCATCCTCACGGTCCATGCAGA TGCCTACCCTAGCATACAGCATTACAACTGGACCTACCTAGGTCCATTCTTTGAAGACCAGCGCAAGCTT GAGTTTATCACCCAAAGGGCCATATACAGGTACACATTCAAGCTCTTTCTGAACCGTGTAAAGGCCTCAG AGGCGGGCCAGTACTTCTTAATGGCACAAAACAAGGCAGGCTGGAATAATCTGACCTTTGAGCTCACCCT GCGATATCCCCCAGAGGTCAGTGTTACATGGATGCCTGTGAATGGCTCTGATGTCCTGTTCTGTGACGTC TCTGGGTACCCTCAGCCCAGCGTGACATGGATGGAGTGCAGGGGCCACACCGATAGGTGTGATGAAGCCC AGGCTTTGCAGGTTTGGAATGACACCCACCCTGAAGTCCTGAGTCAGAAGCCCTTCGACAAAGTGATCAT TCAGAGCCAGCTGCCCATTGGGACCTTAAAACACAACATGACTTATTTTTGCAAAACCCACAACAGTGTG GGTAACAGCTCTCAGTACTTCAGGGCCGTCTCCCTAGGACAAAGCAAGCAGCTCCCCGATGAGTCCCTCT TCACTCCGGTGGTGGTGGCCTGTATGTCTGTCATGTCTCTGCTGGTGCTACTGCTGTTGCTGCTCTTGTA CAAGTACAAGCAGAAGCCGAAGTACCAGGTGCGCTGGAAGATCATCGAGAGATACGAAGGCAATAGCTAC ACCTTCATTGACCCTACTCAGTTGCCCTACAATGAGAAGTGGGAGTTCCCTCGGAACAACCTGCAGTTTG GTAAGACTCTAGGAGCCGGTGCCTTTGGGAAGGTGGTGGAGGCTACAGCCTTTGGTCTGGGCAAAGAAGA TGCAGTGCTGAAGGTGGCTGTGAAGATGCTAAAGTCCACGGCTCATGCTGATGAGAAGGAGGCCCTGATG TCAGAGCTGAAGATCATGAGTCACCTGGGACAGCACGAGAATATAGTCAACCTCTTGGGAGCCTGTACTC ACGGAGGACCTGTCCTGGTCATCACTGAATACTGCTGCTATGGAGACCTACTCAACTTTCTCCGAAGGAA GGCCGAGGCTATGCTAGGACCCAGCCTGAGTCCTGGTCAGGACTCCGAGGGAGACTCCAGCTACAAGAAC ATCCACCTGGAGAAGAAATATGTGCGCAGGGACAGTGGCTTCTCCAGTCAGGGTGTAGACACCTACGTGG AGATGAGGCCTGTCTCGACTTCTTCAAGTGACTCCTTCTTTAAGCAAGATCTGGACAAAGAGGCCAGCCG GCCCCTGGAGCTCTGGGACCTGCTCCACTTCTCCAGCCAAGTGGCTCAGGGCATGGCCTTCCTTGCTTCT AAAAACTGCATCCACCGGGACGTAGCAGCTCGAAACGTGCTGTTGACCAGCGGACATGTGGCCAAGATTG GGGACTTTGGACTGGCTAGGGACATCATGAATGACTCCAACTATGTTGTCAAGGGCAATGCCCGCCTGCC TGTAAAGTGGATGGCCCCAGAGAGCATCTTTGACTGCGTCTACACAGTTCAGAGTGATGTGTGGTCCTAC GGCATCCTCCTCTGGGAGATCTTCTCGCTTGGTCTGAACCCCTACCCCGGCATCCTAGTGAACAACAAGT TCTACAAACTGGTGAAGGATGGATACCAAATGGCCCAGCCTGTATTTGCACCGAAGAACATATACAGCAT CATGCAGTCCTGCTGGGACCTGGAGCCTACCAGAAGACCCACCTTCCAACAGATCTGCTTCCTCCTCCAG GAGCAGGCCCGACTGGAGAGGAGAGACCAGGACTATGCTAACCTGCCAAGCAGCGGTGGCAGCAGCGGCA GTGACAGTGGTGGTGGCAGCAGCGGTGGCAGCAGCAGTGAGCCAGAAGAGGAGAGCTCCAGTGAACACCT GGCCTGCTGTGAGCCAGGGGACATCGCCCAGCCCCTGCTGCAGCCTAACAACTACCAGTTCTGCTGAAGT GGGAGGGAGAGCCGAGTCCTGCCGCTCTCTACGTCCCAGCTTGGCCTCCTCCATGGCACGGGCGACATGG GGAGAACATATGGACTTCGCCCTCAGCTTGGCCCAGCTCTGACACTTCAGAACATGAGGGGTCTGGGGAG GTCAGAGGCCCCGTTTGTTCCCAGAGCCTGGGCCATCACTGCCAGTGGGGTTCTCACAGTGCTAGCCTCT ATATTTACTATGCCAACTGGTGCACCCCTAGTTCTCTTTCTCCATCCTATTCCCATTTTAAAAAACCCGT CCCAAACTCTCGTGTTTCAATGGAAAGACTGATTTATGTCTCAAAAGACAAGAGTCTCAAAGGCTGTGGG TAAGCTGAAGGCTTGCCTCCCTGACAGATGCTTAGACTACAGGCTTCTTGGGACAGGTGGCCCCTTCCTA AGCTCACAGGAGTGGCCACCACTCTTGACCTTCACTCTGTCTATAGTCCCGCCTCATCCTGGATCTTGTA CTGAGCGGCAGCTAAAAGTGTTCTACCCAGTGCCCTGTCACTCTAGACTGGAAGGTATGGGGCCTGATGC AAGGCTGACCACACCAACAAACACCGTGTGCTCCTCTCCAAGCTGACTCGTCCTCATTAACTGTCAACAT TAAACTAACAGCATTAACACAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6418718 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418718 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7647 same embryo
	EMAGE:7645 same embryo
	EMAGE:7644 same embryo
	EMAGE:7646 same embryo
	EMAGE:7643 same embryo
	EurExpress:euxassay_005275 same experiment
	MGI:4824078 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source