EMAGE mouse gene expression spatial database

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EMAGE:7652

Zc4h2 zinc finger, C4H2 domain containing ( MGI:2679294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005296. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005296_01	euxassay_005296_02	euxassay_005296_03	euxassay_005296_04

euxassay_005296_05	euxassay_005296_06	euxassay_005296_07	euxassay_005296_08	euxassay_005296_09

euxassay_005296_10	euxassay_005296_11	euxassay_005296_12	euxassay_005296_13	euxassay_005296_14

euxassay_005296_15	euxassay_005296_16	euxassay_005296_17	euxassay_005296_18	euxassay_005296_19

euxassay_005296_20	euxassay_005296_21	euxassay_005296_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7652_wholemount_strong.wlz

7652_wholemount_moderate.wlz

7652_wholemount_weak.wlz

7652_wholemount_possible.wlz

7652_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7652_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 05 moderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

moderate

regional

moderate expression: see section 05 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 06 07 16 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 16 17 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 07 08 15

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 14

cervical ganglion

moderate

regional

moderate expression: see section 07 08 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 10

dorsal root ganglion

strong

regional

strong expression: see section 09 moderate expression: see section 07 08 12 15 16 17

neural retina

moderate

regional

moderate expression: see section 01 02 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8949
Entity Detected:	Zc4h2, zinc finger, C4H2 domain containing ( MGI:2679294)
Sequence:	sense strand is shown >T8949 AGCCCTTACCACACAGGCTCAAAGCCACAAACCAGTAGGAGGTGGCTGTATTGTATCTATTGTATGCCTT GGCTGGTGCATTTTTTTTTTTAAACAATTCCCTGGACGTGAAATTGAGAGTGAAAAGCATGGCAGATGAA CAAGAAATCATGTGCAAATTGGAAAGCATTAAAGAGATCAGGAACAAGACTCTGCAGATGGAAAAGATCA AGGCCCGTTTGAAGGCTGAGTTCGAGGCTCTGGAGTCTGAAGAGAGGCACCTGAAAGAATACAAACAGGA GATGGATCTCCTGCTACAGGAGAAGATGGCCCATGTGGAGGAACTCCGACTCATCCATGCTGATATCAAT GTGATGGAAAACACCATTAAACAGTCTGAAAATGACTTAAACAAGCTGCTGGAGTCAACCCGGAGGCTTC ATGATGAGTATAAACCACTGAAAGAACACGTGGATGCCCTGCGCATGACATTGGGCCTGCAAAGGCTTCC TGACCTGTGTGAAGAGGAAGAGAAACTCTCCTTGGATTACTTTGAGAAGCAGAAAGCAGAATGGCAGACT GAACCTCAAGAACCCCCCATACCTGAATCCCTTGCTGCTGCAGCTGCTGCTGCTCAACAACTCCAGGTGG CCAGGAAGCAGGACACAAGGCAGACAGCCACCTTCAGGCAGCAGCCTCCACCTATGAAGGCCTGTTTGTC ATGTCACCAACAGATTCATCGAAATGCACCTATATGCCCTCTTTGCAAAGCCAAGAGTCGGTCACGGAAC CCCAAAAAGCCAAAACGGAAGCAAGATGAATGAAGAGAAGGTGGAGGGATAGACCTCTGTTGGTCGTAAA GCCTCTCTTTGGTCAGAGTGATAGATGTTCTGATTTTTTTTTAATATCCCTTCCTTATCCCTACTGAGTC TTCTATTTAATTTGCTGGAAGAATAACCCCTTTCTCCTTGCTCTTATTTCTTTCTGCTCTTGGAAGTTGT GGTTTAAGACCAGCTGTGCTTTGGGTGCTAATCACTGGGACTCATGACCCCTTCTCTCCTAGTCACATTT CTCTTACTGCCCCAGTTTGGAACTGAATGAGGGGGTAAAAGGCCCTAGTTGATACTTTCTTTTGTTCCTG AGGACTGGAGTCTAATGAGCTGTAGGGTGTAAAGACTGGAGGAGCTCCATGCCTTGCTTTGGAAAAAGGC TTCAGTGATTTCCTTCCCTCTGTTTTTGCCTTGGGTTCTGCAGGGACAACTACTTTATTTTTGCTGTACT TCCTAATGGGCTGATGCATATATCTTTCTCAATATTCAGGTCATCATTAAAATCTAGGGGTAACTTAGAC AAGTGTTCCTTTTATATAAAATTGTGAGGCCCTGGTGTTATTCTATAGTTCCACTCAAGTCTTCCAGTTA TACTGAAGTTCTTACATACCAGACAGGGCATCTTCAGAATCGCAAGATCTGTGGATCTTTCTCCATACTT GTATTATTTGCAAATCAGTAGACATTTCCTACTGTGAAATTAGGGACCAAGACCCATGCCACAAGAGTCA CCTAGTTCACTGCCTAAAATACTGGGTCTTATTGCTGTGGTATAGGAATTGTCTTAGACTGAGGAACCCT AAGTGAACTTTAACTTTGAATTTTGTTCAAACCCATTACCTGATCAGTGGTCTATCATTCTGAGGATAGA AAGGGACATAGTGTTATATGAATGCCAATCATTTTATACATGTAAGTAGGTAGGACTTTTGCTTTTCTAT TCTTTCCTCCTTTTGTCTTTTATGAGGTAGCGTCAGTTAAGGACAGTTGGTCACTCCTTTTCTGTGGGTT ATGTAGGTGACCTTTCTTCCACAAATAAAAGACACTTAAGCTCTCCATGCCCCACATCTCATTCCTTTAT CAAAGGTAAGCCTCCTAGTCATGTCTTAGATCTACAGGCTGAAATCTAGAGCTGACTGATGAAAAAGAAT TAGCTCCAGGACTCTATCCCTGCCAGGGGAGAAGAGGGAGCTATTTATCCTATTTTTCTTTAACTTTTGT TTTATGTTAACATATTATATCTTCAAAACATCAATGATTCCTCTGCCTTTTGAAAGTAAAACTGTTATGA TATGATTTCATAAATCCTTTTAAATAAATGTTCATTTGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6807437 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6807437 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7654 same embryo
	EMAGE:7651 same embryo
	EMAGE:7655 same embryo
	EMAGE:7650 same embryo
	EMAGE:7653 same embryo
	EurExpress:euxassay_005296 same experiment
	MGI:4829269 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source