EMAGE mouse gene expression spatial database

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EMAGE:8181

Stx8 syntaxin 8 ( MGI:1890156)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007436. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007436_01	euxassay_007436_02	euxassay_007436_03	euxassay_007436_04

euxassay_007436_05	euxassay_007436_06	euxassay_007436_07	euxassay_007436_08	euxassay_007436_09

euxassay_007436_10	euxassay_007436_11	euxassay_007436_12	euxassay_007436_13	euxassay_007436_14

euxassay_007436_15	euxassay_007436_16	euxassay_007436_17	euxassay_007436_18	euxassay_007436_19

euxassay_007436_20	euxassay_007436_21	euxassay_007436_22	euxassay_007436_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8181_wholemount_strong.wlz

8181_wholemount_moderate.wlz

8181_wholemount_weak.wlz

8181_wholemount_possible.wlz

8181_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8181_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9091
Entity Detected:	Stx8, syntaxin 8 ( MGI:1890156)
Sequence:	sense strand is shown >T9091 GCGGGCGGAGACTGCACCATGGCCCCGGACCCCTGGTTCTCCACATACGATTCTACGTGTCAGATAGCCC AAGAAATCGCTGAGAAGATTCAAGAACGAAATCAGTGTGAAAGAAGAGGTGAAAAGACACCGAAGCTTAC CTTGACAATCAGAACGTTGTTGAAGAATCTGAAGGTAAAGATCGACCTTTTGAAGGACTTACTGCTAAGA GCTGTGTCGACACGCCAGATAACACAACTGGAGGGGGATCGAAGACAGAACCTGCTGGATGACCTTGTCA CCCGAGAGAGACTGCTCCTGGCATCGTTTAAGAATGAGGGTGCAGAGCCGGATCTGATCAGGTCCAGCCT GATGAGCGAAGAAGCAAAGCGAGGAACTCCCAACCCTTGGCTCTGTGAGGAGCCGGAGGAGACCAGAGGT TTGGGTTTTGATGAGATCCGACAACAGCAGCAGAAAATTATTCAAGAACAGGACGCGGGTCTTGATGCCC TTTCCTCTATCATAAGTCGCCAAAAACAAATGGGCCAGGAAATTGGGAACGAACTGGATGAACAGAATGA GATCATCGATGACCTTGCCAACCTCGTTGAGAACACAGATGAGAAGCTTCGAACTGAAGCCAGGCGAGTG ACCCTGGTGGACAGAAAGTCAACTTCCTGTGGGATGATCATGGTGATTTTATTGCTGCTCGTGGCTATTG TGGTCGTTGCAGTGTGGCCAACCAACTGATAGCAATAAAGGGACCACCCGCTGTGACACCTGCCAACTAC GAATGAAAGCCCAGCATCCTTTTGGTACATAACACCTGCTCTCAATAAATTTTCCACCGCTCTGGGCTCT TGGTGTACCTTGTTTGGGTAGAGAACAGAGCTGAGCAGCTCTGGAGACCTTCCTAGAGCACTGGCCCAGT TGTGTCCCCTGTCTTCTGTCAGTGATTTTGTTGGTAGTCAGAAGTCCCTCAGATGGCCATTGTCCCACAG CTGCCAATGGATTCCAGTCACCCTCTGTGTGCTGGGCATCCTGAGGGCTGTCATATTAATAGGCAGTGTG TGACCTTGGTGGCTGCTTGTACTGCGGAAAACCACGTGCCCTTGTGCAAATGAGTGGCTGATCATGTGTC TGGGTGTCCAGATAGCAGGATCTACGTAAGGCAAGAGCCACACAGAAGACATAGCCAACAGCCTCTCTGT CAGCCACCATGTGTAATTTCCCACAAAGCTTAGATGCCCAACAGTGCTGGCTATTTCTGCTCTAAGAGTT TCTGGACCATCTTCGTATCTGGACGTGAACCTTTTCAATTTTGCTTTGTTAATAAACGCGTCCTTCAAGA TTGATGACACCTTTCTAAAACAAAATCCAGACGTGGAGGCACATGCCTTTAACCCCAGCACTTAGGAGTC AGAGGCAGTGGAGTTCAAAAAAAAAAAAACAACAACAACAAAAGACATATTTGACACCTTTCTAAGTAGC CCACAGCCTTAGATCTTGAGGGAGGGAGCAGTCAGAGGAAAGCCTGGTTATAAAGCAGGAAGTGCTGCCT CCGTCCAGCCTCTCCACTGGCTCTGAAGCCATGTGTGTGCCAAGTGAGGGCATGCACATAAGTATTTCAG AGACAGGACTCAGAGCGAAACTGACTGCAAAAGATACCGGGTCTACAGATGGGTGCTGGGGCAGCGAGCC CTCTCTCCTCTCTCTACAGAAAGCAGGATGGGCAGTTAGCCTCGCTTTCTCATAGCAGAAAGAAGAGTTA AAGCTAATGCCAGAAGTCTTGCCGTAGCATTGGCTTCAGTGAGGAGGAGACAGGCCTGTGGTAGGTCCTG TGACTTTCCTTAAGAGAACATAATCCAAAGCCTGCGCTGGACAGAATCAGGCACCAGGAAGAAAGCCCCA GTGTCACCTAGACTGGTGCCCTTTCCTGGGACCCTGAGACCGAGGATTCCAAGTGTTTTGTTTGACTCTG ACTCATACCAGAATGCAGACAGGCAGGACAGCAATGGGGTCTCTGTGGCACAGCAAAGGGTTTCTGGGAG CTCTCAGGCCAGCAGTGAAGTGTCCATCTCGAGAGCACATCACCTCAGCTCGAGTTCTGGCTGAACTACT TACCCTGGCCTCCCACGGGACAAGACGAAGTAACAGAAGACAAGAGAGTAAGAAACACGCTGCCGACGGG GGTTGCCATACCACTTATCAGCTCTGTGACCACAGACAGACAGGTTCCCTTTCTGTGCCAGTGGCCTCAT GTACAAAAAGGTGACAATCCCCCGCTGCCCCACCTTGCCCCACCTCAGGGCTGCTGGGAGGACTGGGTGA GGTACTGAAGCACAGCAAGCCACTTGGCTCAGGTGGAAGGGGACTCTTCCTATAACACTAGTCTAAGCCG AGGTGTCCAGCAGGCTCAGGACCCTGTCTCCCACTCTTCTAAGTACACGAACTGTAGGAGCAAGCCAGGC AGGGGCAAAGTTTGCTGCTAACTTGGCCAATGATGCCCAAGTTCCTGAGGATCCCAAGGCCCTAGGAGCT GACTTAGCCAGATTGTCAGGGTCATAGTATACCAGGAGGTGGCAACTGGCAGAGGGTGTGAAGCAGGCTG TGAGGGGCGGACTGGAGGTGAAGAAGAGTGTGGGCTTGTGTCAGGGGCGTGTGATTGGGAAGACAGGAGC CTTGCTGGACTGCTGGCCAGGGCTTTAAGACACAGTGTACCCTGAGACACATTTGTCAGGGCAGGGTGGT TGCTAATGGATCATCCAATGTAAGACCAAGAATCTGTAGAGCTCCATCATCCAGTCTTGTGGGAAACCCC AAATCTGTACAAACAGTGTCTCCTGGGAGCCCCCCACTTTGGGGCTCAGAAGCAGCAGCATTGAGAGTCT GTTTAACTTTCTCACCTCCTCTTGACTTGCTCAGCACGAAGCCCCGGCAACTCGTGGGACACTTTGAACC AGAGCCACGAAGGCCTGGCTCAGTTGCACTGAAAGCCCTTTTCAGGAAGACACTGGCCCATGTCTGCACA CAGTGGCTTCTGTTCTTCTGGCCGAATGGGCTCTAGGCCCAGTCAGGGCATTGTGTGACCCCGGACAAGT TCCTTCCCTTCTGTATACCATGGGCAGGGGAGCGCAGGAGGAAAGCTGCCTGAGAGGTTCATGGTGTGAG GACCGTGGGCACTGCAGGCAGGGAGACTCATCCCCAGGCAGTCTTCCTCAAAGATGGCTTTCCCCGCACC ATCCAAAGCAGCCAGGCATGCCCAGCTGAGTGGGGTGTACCAGAGAGCCTGCTGCTTCAAAGCCCAGGGG ACAGCTCCTGTAGAGTGGGGTTTGCCAGCGGTGTTTGCAAGGCCTAGTTTCCCAGTTACTATGTCAGGTC TGTAGACCAGTGACCTGCTGTGAGCGTTCTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGAC CTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCATT CTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGAC CTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCATT CTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGAC CTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCGTT CTGGAACCAGTGACCTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCGTTCTGGAACCAGTGAC CTGCTGTGAGCATTCTGGAACCAGTGACCTGCTGTGAGCTTTCTGGAACCAGTGACCTGCTGTGAGCGTT CTAGAAGTACACCACTCACAGAACACAGAGTGGCCAGGCAGCCAGAGCACCCCTGTCTTCAGGGACTTTG TCAATGAAAGTAAACCTTATTAAAACAGCAGCATATGAATTGTTTAACTTAACTGATTATTAATTATATG CATCTTAAATCTGGATACCATATAAAGAAATCCAGATACCTGGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6821351 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6821351 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8182 same embryo
	EMAGE:8180 same embryo
	EMAGE:8183 same embryo
	EMAGE:8184 same embryo
	EMAGE:8179 same embryo
	EurExpress:euxassay_007436 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source