EMAGE mouse gene expression spatial database

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EMAGE:8182

Elavl2 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) ( MGI:1100887)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005246. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005246_01	euxassay_005246_02	euxassay_005246_03	euxassay_005246_04

euxassay_005246_05	euxassay_005246_06	euxassay_005246_07	euxassay_005246_08	euxassay_005246_09

euxassay_005246_10	euxassay_005246_11	euxassay_005246_12	euxassay_005246_13	euxassay_005246_14

euxassay_005246_15	euxassay_005246_16	euxassay_005246_17	euxassay_005246_18	euxassay_005246_19

euxassay_005246_20	euxassay_005246_21	euxassay_005246_22	euxassay_005246_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8182_wholemount_strong.wlz

8182_wholemount_moderate.wlz

8182_wholemount_weak.wlz

8182_wholemount_possible.wlz

8182_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8182_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 21 22 moderate expression: see section 20

vagus x ganglion

strong

regional

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18 19

trigeminal v nerve

strong

regional

strong expression: see section 09 16

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 14 15

cervical ganglion

strong

regional

strong expression: see section 08 09 16 17

thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 14 15 16 17

retina

strong

regional

strong expression: see section 01 02 03 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 13 14 15 16 17 moderate expression: see section 08 09 10 11

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13

pharynx

strong

regional

strong expression: see section 11 14

esophagus

strong

spotted

strong expression: see section 12 13

tongue

strong

spotted

strong expression: see section 14

stomach

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09

midgut

moderate

spotted

moderate expression: see section 08 09 10 11 12 13 14 15 16

testis

strong

spotted

strong expression: see section 05 06 17 moderate expression: see section 04 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9093
Entity Detected:	Elavl2, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) ( MGI:1100887)
Sequence:	sense strand is shown >T9093 GCAGCTGCTAGCAGCAGCCTGGGCTGCAGCAGCTACTAAGCCCAGACCTCAAGTGGATGGATGACTCTGG AAGTGTTTTTCTGAAGGAACCCGCCAGACTTTGGAAGACGGTAGCAGGGCTAGAGAACTTAGATGGGGCT TTCCTGTTGTGCACTTGAACTGGCTGGAGAGATACCTTAGTCCTGCCTTCCCAGTTCTGCGCCCAGCCAA AGCAGTTTTGAAAAATTAAGTGCAAAAAAGCATGACAAAGCAATATGAGGTTGCTGTGCAGGTAATTGCT GCCATGGAAACACAACTGTCTAATGGGCCAACTTGCAATAACACAGCCAATGGTCCAACCACTGTAAACA ACAACTGCTCCTCACCAGTTGACTCCGGGAACACAGAGGACAGCAAGACCAACCTAATAGTCAACTACCT TCCTCAGAACATGACACAGGAAGAACTAAAGAGTCTTTTTGGGAGCATTGGCGAGATAGAGTCCTGTAAG CTTGTAAGAGACAAAATAACAGGGCAGAGCTTGGGTTACGGATTTGTGAACTACATTGACCCCAAGGATG CAGAGAAAGCTATCAACACTCTGAATGGATTGAGACTTCAAACCAAAACAATAAAAGTTTCCTATGCTCG CCCAAGCTCAGCCTCAATCAGAGATGCAAACTTATACGTCAGCGGGCTTCCAAAAACCATGACCCAGAAG GAGCTGGAACAGCTGTTTTCACAGTATGGGCGCATCATTACTTCTCGGATTCTTGTCGACCAGGTCACTG GCATATCAAGGGGTGTAGGGTTTATTCGATTTGACAAGCGGATTGAGGCAGAAGAAGCTATCAAAGGCCT AAATGGCCAGAAACCTCCTGGTGCCACAGAGCCAATCACTGTAAAGTTTGCTAATAATCCAAGCCAAAAA ACCAATCAGGCCATCCTTTCCCAGCTGTACCAGTCTCCAAACAGAAGGTACCCAGGACCGCTAGCTCAGC AGGCACAGCGTTTTAGGTTGGACAATCTGCTCAATATGGCTTATGGAGTGAAGAGGTTTTCTCCAATGAC GATCGATGGAATGACCAGTCTGGCTGGAATTAATATTCCTGGGCACCCTGGAACAGGCTGGTGCATATTT GTGTACAACCTGGCGCCTGATGCAGATGAAAGTATCCTGTGGCAAATGTTTGGGCCTTTTGGAGCTGTCA CCAACGTGAAGGTCATCCGTGACTTTAACACCAATAAGTGCAAAGGTTTTGGATTTGTGACTATGACAAA CTATGATGAGGCGGCCATGGCGATAGCTAGCCTCAATGGATACCGCCTGGGAGACAGAGTCCTGCAGGTC TCCTTTAAGACAAACAAAACGCACAAAGCCTAATGAGCTCTTGTCCTCAGTCCATTTATATATGAAAACT ATACAACAAAAGCAAGTTAAGAGAAACTTTATACATTAGTAAATGTCTTTGTAAGTCAGTGTTGAGATGG GGATAAATGACTACTTAGCATCCTAAGAAATATGTGAGATTTTTTATTGCTAGTATTTGAATTAAAACTT CTTAAATATCTTTTATGTTTGAATATGGACAAGAGGTACAGGGTTTTTACCTGTCACATTGCATTCTATT GCCTTCTTTGAAGAAGGTGGACCTTTTAAAGTGTTTCAGCTAAGGGAAGACATTTCTTTTCTTTTTACAT AACTGCCTTGATCCTGTGAGTAAGTACTGAGGCTTTGTGTTGTAGCTCTTCAGTTGGTTGTCTTTCCCCA CCTCCTTATCTTTCCTTTTCTTTCTCTTTCTTTTGTCTTTGTTTTTTCCTTTCCATTCTTTAATTAGCTT TGTGTTTGGTTTACATTTCAAGCATTGCTGTTATGTCTCAGAAAAGCATTTTGAAGTTTACATTTTTATT TATGAAGTTACAAACAGTATTTATTTTGTAATTCTGATTTGGGCTGGGGCAGGGGGGGCGACATTATAAA CGCTTATTGTAAGAATACTGGAGAACGTTTCGTAAAGCAGTACCTTGCCAAAGAGATAAGAGCCTCTTTG ATGTGGGTTTAAAAAAAGCATCTATTTTTATAAAAAAGAAAATTTGGAGAAACTTTTTACTGGTCCTGGA ACAAATATTTTGACTTGAATACTTTGAGAAATCTCTTCATATGACACCTAGTGAGCTTTTAAAATTTACC AGGAAATTTGCAACGGTTGGAAAATTTAGAAAGATTTATGATGTAGAAAAATACTTTTGAGATCTTTGTA TGAGAGGAATTGAGTCAGTGAGGGAACACTGCTGGTTTCATTTTCGTAATCACCAGTGGGGCGTCTGATC ATCCTGGTTGTTATGTGATAGATGGCTCACATTGATTTGTGATTTTGAAACAAATAAAAAAAATTTACAA AAGAATATATAAGAGCAGGCAAGAAATTTAAATTACCAAGAGATGGGGGAAAATCTGTTCTTCCTAAAGA AATCCCTTCAGATAGAGCTCATGGTGTTTAGTGATGTACTTGCAGTATCGTTTGAAGAATTGTTTTGTCT TAAGGAAGATGAGATGTTGCACACGTTTGGATGGCAGCAAGTCAGCAAAGGCGACCTGAGTTGGATATAT TTGGAGGTATTTTGAGAGTTACGTTTAAGGCTAACACCTGAGCTTTGTGTGATGTAAATAAGACCTTGTG TTTATGAACCTTTCAGCTAATTTTATTTTTTTCCCTTACATATCAAGTGATGTTCAGGTTTTGAATGTTT TGTATCAGTTCTTTTCTTTGTAAATGGCATTAACATTGTTACTTGAGGTCTTGCTTATTCAGTTCTGTTG TCCTGAGGACTTGAATTTACAGTGCATCAGATTCGTTGGCTAATTCTGTCTGTAGATAGTCTAGCTTCAG CTGTTTATGGTGATGCTACATTTTCGTTTATAAATATGTTTGTGGTAAAAAAAAAAGAAAAAAATGAGTA TAACCATAGGTTTTGAACAAATTTCCTTACATTTTTCATACAAAAATCATAAATATCTGTATGCTATTGA AATTTAACTTTGTATGATGCTTAAACCACTATTTGGGGAAATAATAAAATAAGTCTTTACCATGTATGAA AGAAATTTTAAAAAATACAAAATATTTTCTGATTAGCATCTAGCTTATAATAAATTTTCAAAAAAGCTGA AGGCAACTGTGGCCTTCATCAGGATGCACTGAGAACTATATAGTTACGTCCTGCGTTTTGTATAAACTGA GATGCTCATGTGCTTCCCCTTAGAACAGGCAATGTGCTATGCATAACATAGTTGTACATTATCTTTGCAG TTGCTTTGAGTTTTATTTTTTATTATTTAAAACTGTAGTTATAAAAATTTTCAGTATAGTACAGTACATA TACTGTGAGGCGCGTGCTAAAGTGAACAAGCGAGTTTTCATGCTGACCCACTCAATGCTATTCAGAACTC AATTGGCTTAGCACTTTCTCATATCCTTAGGTGCATTTAGATTGTCAGAGTTAACCTGCGTTAAAAAAAA ATAAAATAAAATACATGTATATATTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6821133 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6821133 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8180 same embryo
	EMAGE:8183 same embryo
	EMAGE:8184 same embryo
	EMAGE:8179 same embryo
	EMAGE:8181 same embryo
	EurExpress:euxassay_005246 same experiment
	MGI:4824517 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source