EMAGE mouse gene expression spatial database

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EMAGE:11685

Ctso cathepsin O ( MGI:2139628)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006855. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006855_01	euxassay_006855_02	euxassay_006855_03	euxassay_006855_04

euxassay_006855_05	euxassay_006855_06	euxassay_006855_07	euxassay_006855_08	euxassay_006855_09

euxassay_006855_10	euxassay_006855_11	euxassay_006855_12	euxassay_006855_13	euxassay_006855_14

euxassay_006855_15	euxassay_006855_16	euxassay_006855_17	euxassay_006855_18	euxassay_006855_19

euxassay_006855_20	euxassay_006855_21	euxassay_006855_22	euxassay_006855_23	euxassay_006855_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11685_wholemount_strong.wlz

11685_wholemount_moderate.wlz

11685_wholemount_weak.wlz

11685_wholemount_possible.wlz

11685_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11685_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

weak

regional

weak expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 11 12 13 14

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 06 07 08 09 10 11 16 17 18 19 20 weak expression: see section 12 13 14 15

4th ventricle

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 16 17 18 19 20 21 weak expression: see section 03 04 12 13 14 15

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 12 13 weak expression: see section 10 15

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 18 19 20 21 22 moderate expression: see section 08 09 10 11 12 13 14 15 16 17

pons ventricular layer

moderate

regional

moderate expression: see section 11 12 13 weak expression: see section 08 09 10 14 15 16

midbrain ventricular layer

moderate

regional

moderate expression: see section 11 12 13 weak expression: see section 10 14 15

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8198
Entity Detected:	Ctso, cathepsin O ( MGI:2139628)
Sequence:	sense strand is shown >T8198 GGCACGAGGCCTCGGGCACCGGGATGAAGCCGCAGTTGGTGAACCTCTTGCTACTGTGTTGCTGCTGTCT CGGCCGCCACGGGGTCGCCGGGACCTGGTCTTGGAGCCACCAGCGGGAGGCCGCCGCCTTGCGGGAAAGC CTTCATAGACACCGATACTTGAACTCTTTTCCTCATGAAAACTCCACTGCCTTCTATGGAGTAAATCAGT TTTCTTATTTGTTTCCTGAAGAGTTTAAAGCTTTGTATTTAGGCAGCAAATATGCCTGGGCTCCCAGATA CCCAGCAGAAGGACAGAGACCTATCCCCAATGTGTCTTTGCCATTGAGATTTGACTGGAGGGACAAGCAT GTTGTGAATCCTGTAAGGAACCAGGAGATGTGTGGAGGATGCTGGGCCTTCAGCGTGGTGAGTGCCATAG AGTCAGCTCGTGCAATCCAAGGGAAATCTCTGGATTACCTGAGCGTGCAGCAGGTCATCGACTGCTCCTT TAATAATTCTGGCTGCCTCGGTGGCTCTCCTCCCTGTGCCTTGCGCTGGCTAAATGAGACACAGTTGAAG TTGGTGGCAGATTCACAGTACCCATTCAAGGCAGTAAATGGCCAGTGTCGCCACTTTCCTCAATCACAAG CTGGAGTTTCAGTCAAGGATTTTTCTGCATATAACTTCAGGGGCCAAGAGGATGAAATGGCCAGAGCACT TCTGTCCTTCGGCCCTTTGGTTGTGATAGTTGATGCGATGAGCTGGCAGGATTATCTGGGGGGCATAATC CAACATCACTGCTCCAGTGGGGAGGCCAACCATGCCGTTCTCATCACAGGCTTTGATAGGACAGGAAATA CACCATATTGGATGGTGCGAAACTCCTGGGGATCCTCCTGGGGAGTAGAAGGCTATGCCCACGTGAAAAT GGGAGGTAATGTTTGTGGCATTGCAGACTCTGTAGCTGCTGTCTTTGTGTGATCATGAATTGTTTTGGAT TCACCATGAGACAACTACGAAAATGATGCACTTCATAGTGTGATCCAACATCATACTCAACTCTAAGCAT TATGCACCTTCAAGTTCCAACAATTTGTCTACAGAGAGGTTCTAAGAGCAAACTGGCATTTTGTAAAGCA ACCTAGGACATTCTATTTTTTGCAAAGACATGGACAGCCAAACTTCATAGGAAATCCCCAGGACGTTGAC TGATCAACGGACTCTGTGGGATAGTGTGCACCTCTGAGAGTTTGGTGGGATCAGAAGTCACATAGTATTC TCCTCCTGGAAGCAGTTGGGACTTTTGCTTGCTTGTTGTCTTTCTGGCTGTCTACATTAAGGAAGTATTT TGGTCAGGGTGAATTGAAGATATGATGAGCCTTTGTTTTTCCCTTGTGACTTATAATCACCAGTCTTAGA TTACCTCATCTACAAGAACTCTCAACCTGATGCAATGTGTGCCTCATAATTAGAATGGCCTTCTGTTCCA TGTGAACAGATTTGGAATATCAGTTGTTTTATTTCAGCAAATGGAACTTACTCTAGACAGTAGAACAAAT GTGCACCAAGATGAAACAACGATGCACAAAGAGAATCCCATCTTCAGAGAGACAATCCAACTGGAGTTAA ATAAACACGTGACAACAGTGTGATAAGGAGTACACTATGCCACTTCCAGGGAGTCATGATGTCTATAAAG CTTCACTTTTAAACACCCAAAATGTTTAGCTTCTTGCCTTCTTCCCTCTGAGGTAGAGAATGAAGTATTG TCCACATTTATTCCCTCTCTTGTCCTTTATGGTACAAGTCCTATGGTTTCCTGGTGGTTTACCTTCCAGT CCAAAGTAACTCAGTACCGTTGGTTGTATTCCATTTCTGTTACCCAATCTCTTACTTAAAAACAAAATCT CTGACACAGGCTCACATTGCTCCTTGTTTATGTCCTTCATTAGAATACCAGCATCAATAACAAAGCTCCT TACTCTTTTGTCTGTACCAGTGAGAAGCAATCTTGAATGTGCAGATCATGGAGGGTAAAGAAACGGCAGA AGACATTTCTTAAGGTTTTGAATAAAGTAGCCTAATTATCGAAGGAATCCAGGAAGGAATGTAGTTCAGC TATAGTGTTTCTTAATGACTTTCATTATGTTATCAGCAATGTTTCTAATACCCAGTTTCAAATGCAGATA AACTTGAAATTTTCAAAATCACTGTTACTCTTTAAGTATTCTGGATTGATAACATTTATAATAATACAAC CTACATTTGACTTTGAATTAAAAGTAACTTATTCTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1066724 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1066724 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11689 same embryo
	EMAGE:11688 same embryo
	EMAGE:11687 same embryo
	EMAGE:11690 same embryo
	EMAGE:11686 same embryo
	EurExpress:euxassay_006855 same experiment
	MGI:4824115 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source