EMAGE mouse gene expression spatial database

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EMAGE:11689

Car13 carbonic anhydrase 13 ( MGI:1931322)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006767. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006767_01	euxassay_006767_02	euxassay_006767_03	euxassay_006767_04

euxassay_006767_05	euxassay_006767_06	euxassay_006767_07	euxassay_006767_08	euxassay_006767_09

euxassay_006767_10	euxassay_006767_11	euxassay_006767_12	euxassay_006767_13	euxassay_006767_14

euxassay_006767_15	euxassay_006767_16	euxassay_006767_17	euxassay_006767_18	euxassay_006767_19

euxassay_006767_20	euxassay_006767_21	euxassay_006767_22	euxassay_006767_23	euxassay_006767_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11689_wholemount_strong.wlz

11689_wholemount_moderate.wlz

11689_wholemount_weak.wlz

11689_wholemount_possible.wlz

11689_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11689_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

anterior abdominal wall musculature

moderate

regional

moderate expression: see section 01 02 03

pectoral girdle and thoracic body wall musculature

strong

regional

strong expression: see section 04 05 06 07 08 09 20 21 22 23 24

adrenal cortex

weak

regional

weak expression: see section 11 12 13 20 21 22

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 18 19 20 21

diencephalon meninges

moderate

regional

moderate expression: see section 01 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon meninges

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

moderate

regional

moderate expression: see section 02 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord meninges

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

inner ear

strong

regional

strong expression: see section 04 05 06 07 08 09 10 17 18 19 20 21 22 23 moderate expression: see section 01 02 03

cochlea

strong

single cell

strong expression: see section 06 07 08 09 17 18 19 20

utricle

strong

single cell

strong expression: see section 04 05 06 07 08 20 21 22 23

esophagus

moderate

regional

moderate expression: see section 14 15

frenulum

strong

regional

strong expression: see section 11 12 13 16 moderate expression: see section 14

stomach proventricular region

moderate

regional

moderate expression: see section 10

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 17 18 19 20 21 22 23 24

maxilla

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 16 17 18 19 20 21 22 23

bladder

moderate

regional

moderate expression: see section 17 18

ureter

moderate

regional

moderate expression: see section 13 14 15 18 19 20 weak expression: see section 12

trachea

moderate

regional

moderate expression: see section 15 16

temporal bone petrous part

moderate

regional

moderate expression: see section 04 05 06 07 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8194
Entity Detected:	Car13, carbonic anhydrase 13 ( MGI:1931322)
Sequence:	sense strand is shown >T8194 AGTGTCCCTGCCACAGGCTCTCCATCGAGGACCATGGCGAGGCTGAGCTGGGGGTACGGCGAGCACAACG GTCCCATTCACTGGAATGAATTGTTCCCTATTGCTGATGGTGATCAGCAGTCTCCAATTGAGATTAAAAC CAAAGAAGTGAAATACGACTCCTCACTCCGACCTCTCAGTATCAAGTATGATCCTGCCTCAGCTAAAATC ATCAGCAATAGTGGCCATTCCTTCAACGTTGACTTTGACGACACGGAGGACAAATCAGTTCTGCGTGGAG GTCCTCTCACTGGGAACTACAGGTTGCGGCAGTTCCATTTGCACTGGGGGTCAGCAGATGATCATGGCTC AGAGCATGTGGTAGACGGAGTGAGGTATGCTGCAGAGCTGCATGTTGTCCACTGGAATTCAGACAAATAC CCGAGCTTTGTAGAGGCAGCTCATGAGTCCGATGGGCTGGCTGTCCTGGGAGTATTTCTACAGATTGGGG AACACAATCCTCAACTGCAAAAGATCACTGATATTTTGGATTCCATAAAGGAAAAGGGTAAACAAACACG GTTCACAAATTTTGACCCGTTATGCTTGCTTCCATCATCTTGGGACTACTGGACATACCCTGGCTCTCTG ACAGTTCCACCTCTTCTTGAGAGTGTCACGTGGATTGTTTTAAAACAACCTATAAGTATCAGCTCTCAAC AGCTGGCCAGATTCCGAAGCCTCTTGTGCACAGCTGAGGGAGAGAGTGCGGCTTTTTTGTTAAGCAATCA CCGTCCACCACAGCCCTTGAAGGGCCGCAGAGTGAGAGCATCTTTCTATTAGCAGTCACCAGTGGGTCAA CCCAATCGGAACACGGAGGTAGCAAATACAACGGAACTCAGACATTCCAATAATGGCTTTTCGAGAATTT CACGTTTGTGGATTCTACTTGTCCAGCACCTCTGGCACGGCAGAGAAAACCAGAAGTCTGAAATAACTTA GCCCATCACTCCTAGATTTGCCCCAATGTATGTCCAATTGCTAAATATGGGATGTAATTAAAATCACAAA GTATACCTGCAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:949505 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:949505 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11688 same embryo
	EMAGE:11687 same embryo
	EMAGE:11690 same embryo
	EMAGE:11686 same embryo
	EMAGE:11685 same embryo
	EurExpress:euxassay_006767 same experiment
	MGI:4823628 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source