EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11688

Ntf3 neurotrophin 3 ( MGI:97380)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006775. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006775_01	euxassay_006775_02	euxassay_006775_03	euxassay_006775_04

euxassay_006775_05	euxassay_006775_06	euxassay_006775_07	euxassay_006775_08	euxassay_006775_09

euxassay_006775_10	euxassay_006775_11	euxassay_006775_12	euxassay_006775_13	euxassay_006775_14

euxassay_006775_15	euxassay_006775_16	euxassay_006775_17	euxassay_006775_18	euxassay_006775_19

euxassay_006775_20	euxassay_006775_21	euxassay_006775_22	euxassay_006775_23	euxassay_006775_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11688_wholemount_strong.wlz

11688_wholemount_moderate.wlz

11688_wholemount_weak.wlz

11688_wholemount_possible.wlz

11688_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11688_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 20 21 22 23 24

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 07 08 11 12 15 16 20 21 weak expression: see section 03 04 22 23 24

inner ear

strong

regional

strong expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

anterior naris

strong

regional

strong expression: see section 13 14 15 16 17 moderate expression: see section 18

external naris

strong

regional

strong expression: see section 13 moderate expression: see section 12 18 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 15 16 17 moderate expression: see section 07 08 09 10 11 12 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8202
Entity Detected:	Ntf3, neurotrophin 3 ( MGI:97380)
Sequence:	sense strand is shown >T8202 GCGCACCGCCCCGCGAGATAGCCCGGCGCAACTTTCTTCCCTCTTTCTTTCTTCCCCTCGTTTTTTCCCA CCGCTGGGTAGTGGCTGCGGCAGGGGTGGGCGAGACTGAATGACCGAACTCGAGTCCACCTTTCTCTTCA TGTCGACGTCCCTGGAAATAGTCACACGGATGCCATGGTTACTTCTGCCACGATCTTACAGGTGAACAAG GTGATGTCCATCTTGTTTTATGTGATATTTCTTGCTTATCTCCGTGGCATCCAAGGCAACAGCATGGATC AAAGGAGTTTGCCGGAAGACTCTCTCAATTCCCTCATCATCAAGCTGATCCAGGCGGATATCTTGAAAAA CAAGCTTTCCAAACAGATGGTGGATGTTAAGGAAAATTACCAGAGCACCCTGCCCAAAGCAGAGGCACCC AGGGAACCAGAGCAGGGAGAGGCCACCAGGTCAGAGTTCCAGCCAATGATTGCAACGGACACAGAGCTAC TACGGCAACAGAGACGCTACAATTCGCCCCGGGTCCTGCTGAGTGACAGCACCCCTTTGGAGCCCCCTCC CTTATACCTAATGGAGGATTATGTGGGCAACCCGGTGGTAGCCAATAGAACCTCACCACGGAGGAAACGC TATGCAGAACATAAGAGTCACCGAGGAGAGTACTCAGTGTGTGACAGTGAGAGCCTGTGGGTGACCGACA AGTCCTCAGCCATTGACATTCGGGGACACCAGGTCACAGTGCTGGGGGAGATCAAAACCGGTAACTCTCC TGTGAAACAATATTTTTATGAAACGAGATGTAAAGAAGCCAGGCCGGTCAAAAACGGTTGCAGGGGGATT GATGACAAACACTGGAACTCTCAGTGCAAAACTTCGCAAACCTATGTCCGAGCACTGACTTCAGAAAACA ACAAACTCGTAGGCTGGCGCTGGATACGAATAGACACTTCCTGTGTGTGTGCCTTGTCGAGAAAAATTGG AAGAACATGAATTGGCATCTGTCCCCACATATAAATTATTACTTTAAATTATATGATATGCATGTAGCAT ATAAATGTTTATATTGTTTTTATATATTATAAGTTGACCTTTATTTATTAAACTTCAGCAACCCTTACAG TATATAAGCTTTTTTCTCAATAAAATTCGTGTGCTTGCCTTCCCTCAGGCCTTTCCCATCTGTTAACCTT GTTTTGTGATTGGGCTCTCGGGAACCCTTCTGTAAAACCTGTGTACACCAGTATTTGGCATTCAGTATTG TCAAGGCCATGACTGTGGTTTCAGTAAACTTTCTTAAAATCGGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1177923 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1177923 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11689 same embryo
	EMAGE:11687 same embryo
	EMAGE:11690 same embryo
	EMAGE:11686 same embryo
	EMAGE:11685 same embryo
	EurExpress:euxassay_006775 same experiment
	MGI:4826815 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source