EMAGE mouse gene expression spatial database

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EMAGE:30986

Slc31a1 ( MGI:1333843)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004933_01	euxassay_004933_02	euxassay_004933_03	euxassay_004933_04	euxassay_004933_05

euxassay_004933_06	euxassay_004933_07	euxassay_004933_08	euxassay_004933_09	euxassay_004933_10

euxassay_004933_11	euxassay_004933_12	euxassay_004933_13	euxassay_004933_14	euxassay_004933_15

euxassay_004933_16	euxassay_004933_17	euxassay_004933_18	euxassay_004933_19	euxassay_004933_20

euxassay_004933_21	euxassay_004933_22	euxassay_004933_23	euxassay_004933_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

strong

regional

strong expression: see section 06 07 08 09 10 11 15 16 17 18 19

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

cervical ganglion

strong

regional

strong expression: see section 08 09 16 17

adrenal medulla

strong

regional

strong expression: see section 07 08 09 15 16

lower jaw incisor

strong

regional

strong expression: see section 11 15

upper jaw incisor

strong

regional

strong expression: see section 10 11 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 14 15 16

thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13 14

diencephalon roof plate

strong

regional

strong expression: see section 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6888
Entity Detected:	Slc31a1, ( MGI:1333843)
Sequence:	sense strand is shown >T6888 GGGGCTTGGTAGAAGTCCGTACCGGACTAGGGGATCCAGTTCTGAGAGGAAGACTAAGGCGGCGGGGCTT ACCCTGTGAAGACTTTTCCACTGAATCTCATCTTTTTCTGGAAAACTGGCAATGAACCATATGGGGATGA ACCATATGGAGATGCACCACCATATGGGTATGAACCACACGGACGACAACATTACCATGCCACCTCACCA CCACCCGACCACCTCAGCCTCACACTCCCACGGTGGAGGGGACAGCATGATGATGATGCCTATGACCTTC TACTTTGACTTTAAGAATGTGAATCTATTGTTTTCCGGTTTGGTAATCAATACACCTGGAGAAATGGCTG GAGCCTTCGTGGCAGTGTTTTTACTAGCAATGTTTTATGAAGGACTCAAGATAGCCCGAGAGGGTCTGCT TCGAAAGTCTCAAGTCAGCATTCGCTACAATTCCATGCCTGTCCCAGGACCAAATGGAACCATCCTAATG GAGACACACAAAACTGTTGGGCAGCAGATGCTGAGCTTCCCCCACCTCCTGCAGACAGTGCTGCACATCA TCCAGGTAGTCATCAGCTACTTCCTCATGCTCATCTTCATGACCTACAATGGGTACCTATGCATTGCAGT AGCAGCAGGGGCTGGGACGGGATACTTTCTCTTCAGCTGGAAGAAGGCAGTGGTGGTGGACATCACAGAG CACTGCCATTGATATCAGACTCCACATATGGCCTTATTAATTGCTGTAGGAAGCATCTTGGGACTGGAAG ATATGATTCCCTTTCCAATCATCCCTTCTCTCTCCTGTCTATACATACACACCTACTGAATAGAAGCCTA GCTGACAGTCTCTGAATAGTGGTTTTTCTAATAAGATGAGATTTTTTTCTCTTGGGAAGTCACCTGTGAG ATGTCTTCTCCCTCATCATCCTGAGCCTTATCTAATCCAGGTAGCTTCCTAGTTAAAGACTTGGTTATCT GCTTCTTTTTACTTCTCCAGGTTTTGATTTGTTTTGTTTTGTTTTATTTGCGGGGGGAGTGGGATACATT TTGTTTTAAACAGATATGTGTATTTGATAGGTGGGATTCTGGGTCAGTGATAAAAAAGGATTGTAACAGG ATTGATGGCACTTAGGGACATCTCATCCAACCGGGACTTAAATTTCACACTAGAAATGAGCTCCAGTCTT AACTCAGTGAATGGGAAACACATGTTCCTCTCTCCAGGTTACACTGAAACCTGTGTAGTGTGTACAGAGT GGGTAGGGACTAAACACAGGCCTAGCGTCTATGAGTCTGTATTCCTGCTCAGTCATCAACTTGTGTTCCT ATTGAACATTTGATCAGAATGAACTTGCCAATCAAAACAAAAACAGGACAATCAATTTAAGACAACAGAA ATAGAGATTGTTAAATAAAACCATTAGTATTTTACTTTGACTCAGTACCTTGGTTTGTCCCAACTGAATA AGAGTTTGTATTGTGTGTGTGTTCCTTGAAACCAATGATTTTCAAACTTTACAGCTTCAGAATCACCCAG AGGGCTTGTTAAGACACTACTTGGTGGACCTCAACCCCAGAACCACCAAATTTGGCATTTCTAAGTTCCT AGGTGATGTTCTACTCACAGTCAGTCCAGAGGATTACCTTAAGAGTCTCTGCATTAAACTAGAAAAGTTG CACCTAGAAGGGACAGAGGGTGGGGCTACCTGCTATCAGGCATGAACATGTGAATTGCTGGTTAGTAGGT GGTATGCCTTGGGCACATGTCACATCTAAATGCTGCTGGTCGATACTTTATCATTCCACAAACATTTATT CACTTCCAAATATGTTTTATGTATACCCACATACAGTGTGTGGGATTACAGCCAAAGTATACTTGTCAAC ATATACGAAGTCTCTAGTCCTAACTTTTAAAGATCTAAGGTTCCTTGGAAGTTATTTGAAGGCCAAGAGA CCTTTGCTCTGCTGTGTGAGTCAGCCAGTCAAAAGCCCAGAAAGGCAAAAACCCAGCTTTCTTGTTTGGA GAGACATGGGGCATGTGTCTGCCAATAGCCAGTTGTTCTCAGTGTGCACTATGTAGGTCCCCATCCACTA CTTGACCACCTTTATCATTTACGGGGATTTACATAGATTACAGTGGCCAAGACCCCTTTCTCAATGAAGG ATCTCATTGGACGTCCTGTCAATCATTAATCTACATGCAGGCTTGTTCCCACACTACCTCTTTAAAGCGA TTCTTCCTATGCTGGTCTGTTCAATCCTGCCCTCTTTGGTGCTAGATCCAGTTGTGCCTCAGAGCAGAAG AAACAAACCTGCTGTCTTGTTGGCCCAGTGTTTCAGTTTTGAAGTGTGTACTTTCTCCTCTGTGTGTGCC AATTACATATTAGAGAACAAGGAATGTGTACTTTGGTGGCTTTGAACCAAGAAAAGGTTTTAAGCCTACT GGATGGAGTCTAAAATTCTTAGAAATCAACTTTAGAGCTTTGCTTTTCTCTGAGTTCATCCTGCAGTGAA TGACAGGTACTGACAGCCCAACATTAAGGTGCAAATAGCCTGTTTGTCATATCAGAAGCCATGGAATAAT AAACCCAGGGGAATCCAGAAACCACACTTAGTGGTAACCCTGGTGCTCTCTAGAATCTCCAAATGAGCTG CTATAAAACACCTTTCAGGGGCTGAGATGCAGCTCTGGTGTGAGTCACTTACCTAGCTTATGTCAGGCCC CTAAAAGTAATGCATAAACTGTCAGAATCGGGACCTTATCGTTGAGGTGAAAAGAACCAAGGCCCGTAGG AACAAGGTGCTATGAATCTGAAACCAAGAGAAACCTCAGTTTGGCATGGTTCTTCAGGGTAGCTTCCGAG TAGAAATAGTCTGCTGCAAATCCTAAACTTAAGATCCCTGCGCTAGGTTGCTAGAGACTGCGGTTAGTGC AGAACAGAGGTAAGCCTAAGTCCCATCCTCATGAACTTCAATGCCCAGCCTTTGTGTTTATTTTATCTAA ATGTCTCCTTTCATCTGTGGGAAAGCTATAGCTGAGTGTCTTTAGCCCCTGCCCTACTTTTCCCTCTCTG ACTTTTTTCCCAGGTAGCTTAGTGTTTAACATCTCTCTCACCATCCTGATGCAGTAGCATGGCTATTGCT GAGGTTATTTTACCTAATAATTAGAACTTTGTATTTACAAAGCAAGTAGAGGTCTTAAATTTGCTTCAAG AAGCAGTTGCTCCACTGGAATTTCTATAAAGCTGGGTAAAGGGTTAGCAGAATTACAGATCTTAAGTACT AACTGCAGTCCAAGATGGAAGAGTACTCAGCAGCCACTGCCCTCATCAGGAACCTCCTCGGCATCCATCA GTCTCCTCACTGCTCCTGTGCGCAGGGCTGTCCCTCCCACACTCCCTTCCAGGCCGTGGCCTTCGCTTCC TCATTAGAATCAGGAGAGTGAGGCACAGTGTCCATCATGCCATTTAATCTTGTGCCTGCCCTTTCCAGCC TCTTCAAAAGATGCATCCAAACAACAGGTCTGCATTTGATGTAATGCCTTAATTACTGGGGCCACATTCA CGTTGACTGTCTCAGAGACTGTAAGCTCCCGGAGAGCAGGGTTGCTGTAATGGGAATGTTTAAGTGTTGT GTGCAAGAAAACAAAATAAATATTTTGATTTTCGTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3968084 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3968084 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004933 same experiment
	EMAGE:32054 same embryo
	EMAGE:31598 same embryo
	EMAGE:30662 same embryo
	EMAGE:30640 same embryo
	EMAGE:31995 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source